Cellosaurus logo
expasy logo

Cellosaurus H9EOS (CVCL_C0VX)

[Text][XML][JSON]
Cell line name H9EOS
Accession CVCL_C0VX
Resource Identification Initiative To cite this cell line use: H9EOS (RRID:CVCL_C0VX)
Comments Characteristics: Transduced with a EOS-C(3+)-GFP/puroR construct. EOS (ETn, Oct-4 and Sox2) combines a highly active promoter from a mouse early transposon (ETn) that is specific for undifferentiated pluripotent stem cells and combined it with Oct-4 (Pou5f1) and Sox2-binding motifs. EOS regulates an enhanced green fluorescent protein (eGFP), an internal ribosome entry site (IRES) and a puromycin resistance gene to allow live-cell visualization and antibiotic selection simultaneously.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=25215486; DOI=10.1016/j.cell.2014.08.029; PMCID=PMC4162745
Yasuhiro Takashima, Ge Guo, Remco Loos, Jennifer Nichols, Gabriella Ficz, Felix Krueger, David Oxley, Fatima Santos, James Clarke, William Mansfield, Wolf Reik ...Show all 13 authors... , Paul Bertone, Austin Gerard Smith; Show fewer authors
Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Cell 158:1254-1269(2014)

PubMed=28765214; DOI=10.1242/dev.146811; PMCID=PMC5560041
Ge Guo, Ferdinand von Meyenn, Maria Rostovskaya, James Clarke, Sabine Dietmann, Duncan E.C. Baker, Anna Sahakyan, Samuel Myers, Paul Bertone, Wolf Reik, Kathrin Plath, Austin Gerard Smith;
Epigenetic resetting of human pluripotency.
Development 144:2748-2763(2017)

Cross-references
Encyclopedic resources Wikidata; Q114311558
Entry history
Entry creation22-Sep-2022
Last entry update14-Aug-2025
Version number6