ID   H9EOS
AC   CVCL_C0VX
DR   Wikidata; Q114311558
RX   PubMed=25215486;
RX   PubMed=28765214;
CC   Characteristics: Transduced with a EOS-C(3+)-GFP/puroR construct. EOS (ETn, Oct-4 and Sox2) combines a highly active promoter from a mouse early transposon (ETn) that is specific for undifferentiated pluripotent stem cells and combined it with Oct-4 (Pou5f1) and Sox2-binding motifs. EOS regulates an enhanced green fluorescent protein (eGFP), an internal ribosome entry site (IRES) and a puromycin resistance gene to allow live-cell visualization and antibiotic selection simultaneously.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 22-09-22; Last updated: 29-06-23; Version: 4
//
RX   PubMed=25215486; DOI=10.1016/j.cell.2014.08.029;
RA   Takashima Y., Guo G., Loos R., Nichols J., Ficz G., Krueger F.,
RA   Oxley D., Santos F., Clarke J., Mansfield W., Reik W., Bertone P.,
RA   Smith A.G.;
RT   "Resetting transcription factor control circuitry toward ground-state
RT   pluripotency in human.";
RL   Cell 158:1254-1269(2014).
//
RX   PubMed=28765214; DOI=10.1242/dev.146811;
RA   Guo G., von Meyenn F., Rostovskaya M., Clarke J., Dietmann S.,
RA   Baker D.E.C., Sahakyan A., Myers S., Bertone P., Reik W., Plath K.,
RA   Smith A.G.;
RT   "Epigenetic resetting of human pluripotency.";
RL   Development 144:2748-2763(2017).
//