Cellosaurus cell line C8-B (CVCL

Cellosaurus C8-B (CVCL_B6F4)

[Text version]

Cell line name

C8-B

Accession

CVCL_B6F4

Resource Identification Initiative

To cite this cell line use: C8-B (RRID:CVCL_B6F4)

Comments

Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with the retroviral vector SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.

Species of origin

Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Lewis; RS=RS_0000121.

Hierarchy

Children:

CVCL_B6F5 (C8-B/Ras)

Sex of cell

Male

Age at sampling

Age unspecified