Cellosaurus C8-B/Ras (CVCL_B6F5)
| Cell line name | C8-B/Ras |
|---|---|
| Accession | CVCL_B6F5 |
| Resource Identification Initiative | To cite this cell line use: C8-B/Ras (RRID:CVCL_B6F5) |
| Comments | Characteristics: Tumorigenic. Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype. Characteristics: Transduced with the retroviral vector SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5173; HRAS (Note=With p.Gly12Val). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Hepatocyte; CL=CL_0000182. |
| Species of origin | Rattus norvegicus (Rat)
(NCBI Taxonomy: 10116)
Breed/subspecies: Lewis; RS=RS_0000121. |
| Hierarchy | Parent: CVCL_B6F4 (C8-B) |
| Sex of cell | Male |
| Age at sampling | Age unspecified |
| Category | Transformed cell line |
| Publications | PubMed=11097476; DOI=10.1016/S0168-8278(00)80299-8 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q111733055 |
| Entry history | |
| Entry creation | 17-Mar-2022 |
| Last entry update | 10-Apr-2025 |
| Version number | 8 |