ID   C8-B
AC   CVCL_B6F4
DR   Wikidata; Q111733054
RX   PubMed=11097476;
CC   Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
CC   Characteristics: Transduced with the retroviral vector SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
CC   Breed/subspecies: Lewis; RS=RS_0000121.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
SX   Male
AG   Age unspecified
CA   Transformed cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 7
//
RX   PubMed=11097476; DOI=10.1016/S0168-8278(00)80299-8;
RA   Cai J., Ito M., Westerman K.A., Kobayashi N., Leboulch P., Fox I.J.;
RT   "Construction of a non-tumorigenic rat hepatocyte cell line for
RT   transplantation: reversal of hepatocyte immortalization by
RT   site-specific excision of the SV40 T antigen.";
RL   J. Hepatol. 33:701-708(2000).
//