Cellosaurus YOCK-13 (CVCL_B6ER)
| Cell line name | YOCK-13 |
|---|---|
| Accession | CVCL_B6ER |
| Resource Identification Initiative | To cite this cell line use: YOCK-13 (RRID:CVCL_B6ER) |
| Comments | Characteristics: Expresses an insulin variant which is a single-chain modified insulin which is made of the signal sequence of albumin, the A chain followed by short turn-forming heptapeptide (GGGPGKR) and the B chain. This construct does not require pancreas-specific enzymatic processing (PubMed=14693704). Characteristics: Reversibly immortalized; exposure to tamoxifen induces the expression of the Cre gene that effects Cre/LoxP recombination and excise the TERT gene. Characteristics: Transduced with three constructs: 1) a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites, 2) a plasmid pCAGMerCreMer/Puro that expresses Cre fused to 2 mutant estrogen-receptor ligand-binding domains (MerCreMer) under the control of the CAG promoter and 3) a plasmid vector expressing modified insulin and zeocin resistance genes under the control of the rat L-PK promoter. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6081; INS (Note=Engineered variant). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Hepatocyte; CL=CL_0000182. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_B6EQ (TTNT-16-3) |
| Sex of cell | Sex unspecified |
| Age at sampling | Age unspecified |
| Category | Telomerase immortalized cell line |
| Publications | PubMed=14693704; DOI=10.2337/diabetes.53.1.105 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q112041944 |
| Entry history | |
| Entry creation | 17-Mar-2022 |
| Last entry update | 10-Apr-2025 |
| Version number | 7 |