ID   YOCK-13
AC   CVCL_B6ER
DR   Wikidata; Q112041944
RX   PubMed=14693704;
CC   Characteristics: Expresses an insulin variant which is a single-chain modified insulin which is made of the signal sequence of albumin, the A chain followed by short turn-forming heptapeptide (GGGPGKR) and the B chain. This construct does not require pancreas-specific enzymatic processing (PubMed=14693704).
CC   Characteristics: Reversibly immortalized; exposure to tamoxifen induces the expression of the Cre gene that effects Cre/LoxP recombination and excise the TERT gene.
CC   Characteristics: Transduced with three constructs: 1) a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites, 2) a plasmid pCAGMerCreMer/Puro that expresses Cre fused to 2 mutant estrogen-receptor ligand-binding domains (MerCreMer) under the control of the CAG promoter and 3) a plasmid vector expressing modified insulin and zeocin resistance genes under the control of the rat L-PK promoter.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6081; INS (Note=Engineered variant).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B6EQ ! TTNT-16-3
SX   Sex unspecified
AG   Age unspecified
CA   Telomerase immortalized cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 7
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RX   PubMed=14693704; DOI=10.2337/diabetes.53.1.105;
RA   Okitsu T., Kobayashi N., Jun H.-S., Shin S., Kim S.-J., Han J., Kwon H.,
RA   Sakaguchi M., Totsugawa T., Kohara M., Westerman K.A., Tanaka N.,
RA   Leboulch P., Yoon J.-W.;
RT   "Transplantation of reversibly immortalized insulin-secreting human
RT   hepatocytes controls diabetes in pancreatectomized pigs.";
RL   Diabetes 53:105-112(2004).
//