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Cellosaurus TTNT-16-3 (CVCL_B6EQ)

[Text version]
Cell line name TTNT-16-3
Synonyms 16-T3
Accession CVCL_B6EQ
Resource Identification Initiative To cite this cell line use: TTNT-16-3 (RRID:CVCL_B6EQ)
Comments Characteristics: Reversibly immortalized; exposure to tamoxifen induces the expression of the Cre gene that effects Cre/LoxP recombination and excise the TERT gene.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites and a plasmid pCAGMerCreMer/Puro that expresses Cre fused to 2 mutant estrogen-receptor ligand-binding domains (MerCreMer) under the control of the CAG promoter.
Doubling time: ~48 hours (PubMed=17434229).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B6EN (TTNT-16)
Children:
CVCL_B6ER (YOCK-13)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Telomerase immortalized cell line
Publications

PubMed=14693704; DOI=10.2337/diabetes.53.1.105
Teru Okitsu, Naoya Kobayashi, Hee-Sook Jun, Seungjin Shin, Su-Jin Kim, Jaeseok Han, Hyokjoon Kwon, Masakiyo Sakaguchi, Toshinori Totsugawa, Michinori Kohara, Karen A. Westerman ...Show all 14 authors... , Noriaki Tanaka, Philippe Leboulch, Ji-Won Yoon; Show fewer authors
Transplantation of reversibly immortalized insulin-secreting human hepatocytes controls diabetes in pancreatectomized pigs.
Diabetes 53:105-112(2004)

PubMed=17434229; DOI=10.1016/j.jhep.2007.02.019
Toshinori Totsugawa, Chen Yong, Jorge David Rivas-Carrillo, Alejandro Soto-Gutierrez, Nalu Navarro-Alvarez, Hirofumi Noguchi, Teru Okitsu, Karen A. Westerman, Michinori Kohara, Michael G. Reth, Noriaki Tanaka ...Show all 13 authors... , Philippe Leboulch, Naoya Kobayashi; Show fewer authors
Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with tamoxifen-mediated self-recombination.
J. Hepatol. 47:74-82(2007)

Cross-references
Encyclopedic resources Wikidata; Q112041867
Entry history
Entry creation17-Mar-2022
Last entry update14-Aug-2025
Version number8