Cellosaurus cell line TTNT-16-3 (CVCL

Cellosaurus TTNT-16-3 (CVCL_B6EQ)

[Text version]

Cell line name

TTNT-16-3

Synonyms

16-T3

Accession

CVCL_B6EQ

Resource Identification Initiative

To cite this cell line use: TTNT-16-3 (RRID:CVCL_B6EQ)

Comments

Characteristics: Reversibly immortalized; exposure to tamoxifen induces the expression of the Cre gene that effects Cre/LoxP recombination and excise the TERT gene.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites and a plasmid pCAGMerCreMer/Puro that expresses Cre fused to 2 mutant estrogen-receptor ligand-binding domains (MerCreMer) under the control of the CAG promoter.
Doubling time: ~48 hours (PubMed=17434229).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_B6EN (TTNT-16)
Children:

CVCL_B6ER (YOCK-13)

Sex of cell

Sex unspecified

Age at sampling

Age unspecified