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Cellosaurus TTNT-16 (CVCL_B6EN)

[Text version]
Cell line name TTNT-16
Synonyms 16
Accession CVCL_B6EN
Resource Identification Initiative To cite this cell line use: TTNT-16 (RRID:CVCL_B6EN)
Comments Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B6EQ (TTNT-16-3)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Telomerase immortalized cell line
Publications

PubMed=17434229; DOI=10.1016/j.jhep.2007.02.019
Toshinori Totsugawa, Chen Yong, Jorge David Rivas-Carrillo, Alejandro Soto-Gutierrez, Nalu Navarro-Alvarez, Hirofumi Noguchi, Teru Okitsu, Karen A. Westerman, Michinori Kohara, Michael G. Reth, Noriaki Tanaka ...Show all 13 authors... , Philippe Leboulch, Naoya Kobayashi; Show fewer authors
Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with tamoxifen-mediated self-recombination.
J. Hepatol. 47:74-82(2007)

Cross-references
Encyclopedic resources Wikidata; Q112041866
Entry history
Entry creation17-Mar-2022
Last entry update10-Apr-2025
Version number7