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Cellosaurus TTNT-16 (CVCL_B6EN)

[Text version]
Cell line name TTNT-16
Synonyms 16
Accession CVCL_B6EN
Resource Identification Initiative To cite this cell line use: TTNT-16 (RRID:CVCL_B6EN)
Comments Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B6EQ (TTNT-16-3)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Telomerase immortalized cell line
Publications

PubMed=17434229; DOI=10.1016/j.jhep.2007.02.019
Totsugawa T., Yong C., Rivas-Carrillo J.D., Soto-Gutierrez A., Navarro-Alvarez N., Noguchi H., Okitsu T., Westerman K.A., Kohara M., Reth M.G., Tanaka N., Leboulch P., Kobayashi N.
Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with tamoxifen-mediated self-recombination.
J. Hepatol. 47:74-82(2007)

Cross-references
Encyclopedic resources Wikidata; Q112041866
Entry history
Entry creation17-Mar-2022
Last entry update10-Apr-2025
Version number7