ID   TTNT-16
AC   CVCL_B6EN
SY   16
DR   Wikidata; Q112041866
RX   PubMed=17434229;
CC   Characteristics: Because it has been transduced by a construct containing LoxP sites, transfection with the Cre recombinase eliminates the SV40 T gene thus reverting the cells to a non-immortal phenotype.
CC   Characteristics: Transduced with a retroviral vector SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by a pair of loxP sites.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   Age unspecified
CA   Telomerase immortalized cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 7
//
RX   PubMed=17434229; DOI=10.1016/j.jhep.2007.02.019;
RA   Totsugawa T., Yong C., Rivas-Carrillo J.D., Soto-Gutierrez A.,
RA   Navarro-Alvarez N., Noguchi H., Okitsu T., Westerman K.A., Kohara M.,
RA   Reth M.G., Tanaka N., Leboulch P., Kobayashi N.;
RT   "Survival of liver failure pigs by transplantation of reversibly
RT   immortalized human hepatocytes with tamoxifen-mediated
RT   self-recombination.";
RL   J. Hepatol. 47:74-82(2007).
//