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Cellosaurus Sua-5B-IE8-Act::Cas9-2A-Neo (CVCL_B3N3)

[Text version]
Cell line name Sua-5B-IE8-Act::Cas9-2A-Neo
Accession CVCL_B3N3
Resource Identification Initiative To cite this cell line use: Sua-5B-IE8-Act::Cas9-2A-Neo (RRID:CVCL_B3N3)
Comments Group: Insect cell line.
Group: Malaria research cell line.
Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase.
Characteristics: A N-3xFLAG-spCas9-T2A-Neo/Kan R construct was integrated in the Act5C locus.
Doubling time: ~20 hours (DGRC=334).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Species of origin Anopheles coluzzii (African malaria mosquito) (NCBI Taxonomy: 1518534)
Breed/subspecies: Suakoko 2La.
Hierarchy Parent: CVCL_B3N2 (Sua-5B-IE8)
Sex of cell Sex unspecified
Age at sampling Neonate larva
Category Spontaneously immortalized cell line
Publications

PubMed=34819517; DOI=10.1038/s41467-021-27129-3
Viswanatha R., Mameli E., Rodiger J., Merckaert P., Feitosa-Suntheimer F., Colpitts T.M., Mohr S.E., Hu Y.-H., Perrimon N.
Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.
Nat. Commun. 12:6825.1-6825.13(2021)

Cross-references
Cell line collections (Providers) DGRC; 334
Encyclopedic resources Wikidata; Q110433264
Entry history
Entry creation16-Dec-2021
Last entry update30-Jan-2024
Version number6