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Cellosaurus Sua-5B-IE8 (CVCL_B3N2)

[Text version]
Cell line name Sua-5B-IE8
Accession CVCL_B3N2
Resource Identification Initiative To cite this cell line use: Sua-5B-IE8 (RRID:CVCL_B3N2)
Comments Group: Insect cell line.
Group: Malaria research cell line.
Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase.
Doubling time: ~20 hours (DGRC=333).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Species of origin Anopheles coluzzii (African malaria mosquito) (NCBI Taxonomy: 1518534)
Breed/subspecies: Suakoko 2La.
Hierarchy Parent: CVCL_RQ24 (Sua5B)
Children:
CVCL_B3N3 (Sua-5B-IE8-Act::Cas9-2A-Neo)
Sex of cell Sex unspecified
Age at sampling Neonate larva
Category Spontaneously immortalized cell line
Publications

PubMed=34819517; DOI=10.1038/s41467-021-27129-3
Viswanatha R., Mameli E., Rodiger J., Merckaert P., Feitosa-Suntheimer F., Colpitts T.M., Mohr S.E., Hu Y.-H., Perrimon N.
Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.
Nat. Commun. 12:6825.1-6825.13(2021)

Cross-references
Cell line collections (Providers) DGRC; 333
Encyclopedic resources Wikidata; Q110433263
Entry history
Entry creation16-Dec-2021
Last entry update30-Jan-2024
Version number6