ID   Sua-5B-IE8-Act::Cas9-2A-Neo
AC   CVCL_B3N3
DR   DGRC; 334
DR   Wikidata; Q110433264
RX   PubMed=34819517;
CC   Group: Insect cell line.
CC   Group: Malaria research cell line.
CC   Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase.
CC   Characteristics: A N-3xFLAG-spCas9-T2A-Neo construct was integrated in the Act5C locus (PubMed=34819517).
CC   Doubling time: ~20 hours (DGRC=334).
CC   Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Breed/subspecies: Suakoko 2La.
OX   NCBI_TaxID=1518534; ! Anopheles coluzzii (African malaria mosquito)
HI   CVCL_B3N2 ! Sua-5B-IE8
SX   Sex unspecified
AG   Neonate larva
CA   Spontaneously immortalized cell line
DT   Created: 16-12-21; Last updated: 14-08-25; Version: 8
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RX   PubMed=34819517; DOI=10.1038/s41467-021-27129-3; PMCID=PMC8613219;
RA   Viswanatha, Raghuvir
RA   Mameli, Enzo
RA   Rodiger, Jonathan
RA   Merckaert, Pierre
RA   Feitosa-Suntheimer, Fabiana
RA   Colpitts, Tonya M.
RA   Mohr, Stephanie E.
RA   Hu, Yan-Hui
RA   Perrimon, Norbert
RT   "Bioinformatic and cell-based tools for pooled CRISPR knockout
RT   screening in mosquitos.";
RL   Nat. Commun. 12:6825.1-6825.13(2021).
//