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Cellosaurus HUES 6 (CVCL_B194)

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Cell line name HUES 6
Synonyms HUES-6; HUES6; HuES6; Hues6; HuES06; HVRDe006-A
Accession CVCL_B194
Resource Identification Initiative To cite this cell line use: HUES 6 (RRID:CVCL_B194)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0019.
Registration: Swiss research registry; BAG-hES-IMP-0038.
Registration: UK Stem Cell Bank (UKSCB); R-06-009, Steering comm. appl. SCSC04-25(6).
Doubling time: 24 hours (PubMed=14999088).
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K36me3.
Omics: Genomics; ChIP-seq; H3K4me1.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; ChIP-seq; H3K9ac.
Omics: Genomics; ChIP-seq; H3K9me3.
Omics: Genomics; DNA methylation analysis.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E6IC (HUES6 SOX11 ES.HET1)CVCL_E6ID (HUES6 SOX11 ES.HET2)CVCL_YN14 (HVRDe006-A-1)
CVCL_YN72 (HVRDe006-A-4)CVCL_YN73 (HVRDe006-A-6)CVCL_A9YS (HVRDe006-A-7)
CVCL_A9YT (UKERe006-C)CVCL_A9YU (UKERe006-D)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798; PubMed=21637780; PubMed=28677534

Markers:
AmelogeninX
CSF1PO11,12
D1S165615,18.3
D2S133823,24
D3S135816,18
D5S81812
D6S104311,13
D7S8208,11
D8S117910,13
D12S39124,25
D13S31711
D16S53910,11
D18S5112,14
D19S43313,15
D21S1130
FGA23,24
Penta D9,10
Penta E12
TH018,9.3
TPOX9,11
vWA15,16

Run an STR similarity search on this cell line
Publications

PubMed=14999088; DOI=10.1056/NEJMsr040330
Chad A. Cowan, Irina V. Klimanskaya, Jill McMahon, Jocelyn Atienza, Jeannine Witmyer, Jacob P. Zucker, Shun-Ping Wang, Cynthia Casson Morton, Andrew P. McMahon, Doug Powers, Douglas A. Melton;
Derivation of embryonic stem-cell lines from human blastocysts.
N. Engl. J. Med. 350:1353-1356(2004)

PubMed=17287758; DOI=10.1038/nbt1285
Duncan E.C. Baker, Neil J. Harrison, Edna Maltby, Kath Smith, Harry D. Moore, Pamela Jean Shaw, Paul R. Heath, Hazel Holden, Peter W. Andrews;
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo.
Nat. Biotechnol. 25:207-215(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews, Gemma Beighton, Paul A. Bello, Nissim Benvenisty, Lorraine S. Berry, Simon Bevan, Barak Blum ...Show all 85 authors... , Justin Brooking, Kevin G. Chen, Andre B.-H. Choo, Gary A. Churchill, Marie Corbel, Ivan Damjanov, Jonathan S. Draper, Petr Dvorak, Katarina Emanuelsson, Roland A. Fleck, Angela Ford, Karin Gertow, Marina Gertsenstein, Paul J. Gokhale, Rebecca S. Hamilton, Ales Hampl, Lyn E. Healy, Outi Hovatta, Johan Hyllner, Marta P. Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jacqueline L. Johnson, Mark Jones, Kehkooi Kee, Benjamin L. King, Barbara B. Knowles, Majlinda Lako, Franck Lebrin, Barbara S. Mallon, Daisy Manning, Yoav Mayshar, Ronald Davies Graham McKay, Anna E. Michalska, Milla Mikkola, Maria Mileikovsky, Stephen Lewis Minger, Harry D. Moore, Christine L. Mummery, Andras Nagy, Norio Nakatsuji, Carmel M. O'Brien, Steve Kah-Weng Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal J. Patel, Roger Arnold Pedersen, Martin F. Pera, Marian S. Piekarczyk, Renee A. Reijo Pera, Benjamin E. Reubinoff, Allan J. Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C. Schulz, Henrik Semb, Eric S. Sherrer, Henrike Siemen, Glyn N. Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Tako Turetsky, Timo Tuuri, Stieneke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward-van Oostwaard, Thomas A. Weaver, Lesley A. Young, Wei-Dong Zhang; Show fewer authors
International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001; PMCID=PMC3335201
Alice E. Chen, Dietrich Meinrad Egli, Kathy K. Niakan, Jie Deng, Hidenori Akutsu, Mariko Yamaki, Chad A. Cowan, Claire Fitz-Gerald, Kun Zhang, Douglas A. Melton, Kevin Carl Eggan;
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032; PMCID=PMC3063454
Christoph Bock, Evangelos Kiskinis, Griet Verstappen, Hong-Cang Gu, Gabriella L. Boulting, Zachary D. Smith, Michael Johannes Ziller, Gist Fralley Croft, Mackenzie Weygandt Amoroso, Derek H. Oakley, Andreas Gnirke ...Show all 13 authors... , Kevin Carl Eggan, Alexander Meissner; Show fewer authors
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085; PMCID=PMC3102737
Koichiro Nishino, Masashi Toyoda, Mayu Yamazaki-Inoue, Yoshihiro Fukawatase, Emi Chikazawa, Hironari Sakaguchi, Hidenori Akutsu, Akihiro Umezawa;
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:e1002085.1-e1002085.14(2011)

PubMed=28677534; DOI=10.1016/j.scr.2017.03.015
Maike Marczenke, Jakob Fell, Ilaria Piccini, Albrecht Ropke, Guiscard Seebohm, Boris Greber;
Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation.
Stem Cell Res. 21:26-28(2017)

Cross-references
Cell line databases/resources hPSCreg; HVRDe006-A
NIHhESC; NIHhESC-10-0019
SKIP; SKIP001484
SKIP; SKIP001910
Biological sample resources BioSamples; SAMEA7771168
ENCODE; ENCBS153ORV
ENCODE; ENCBS271TBU
ENCODE; ENCBS530IZG
ENCODE; ENCBS912XKA
Encyclopedic resources Wikidata; Q54896798
Experimental variables resources EFO; EFO_0007086
Gene expression databases GEO; GSM525424
GEO; GSM525425
GEO; GSM525426
GEO; GSM608012
GEO; GSM621700
GEO; GSM637760
GEO; GSM669885
GEO; GSM669886
GEO; GSM669887
GEO; GSM669888
GEO; GSM669889
GEO; GSM669890
GEO; GSM669891
GEO; GSM669892
GEO; GSM669893
GEO; GSM669894
GEO; GSM669895
GEO; GSM669897
GEO; GSM669898
GEO; GSM670080
GEO; GSM1112774
GEO; GSM1112776
GEO; GSM1153528
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number25