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Cellosaurus MDA-MB-435-LCC15 (CVCL_B055)

[Text version]
Cell line name MDA-MB-435-LCC15
Synonyms LLC-15MB; LCC15-MB
Accession CVCL_B055
Secondary accession CVCL_1U14
Resource Identification Initiative To cite this cell line use: MDA-MB-435-LCC15 (RRID:CVCL_B055)
Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative (PubMed=20143388). Was first thought to originate from the femoral metastasis of a breast carcinoma but was later shown to be contaminated by MDA-MB-435 (PubMed=15679051).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00231.
Population: Caucasian.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0417 (MDA-MB-435)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx

PubMed=9637769; DOI=10.1006/excr.1998.4029
Sung V., Gilles C., Murray A., Clarke R., Aaron A.D., Azumi N., Thompson E.W.
The LCC15-MB human breast cancer cell line expresses osteopontin and exhibits an invasive and metastatic phenotype.
Exp. Cell Res. 241:273-284(1998)

PubMed=10432004; DOI=10.1023/A:1006598422203
Thompson E.W., Sung V., Lavigne M., Baumann K.H., Azumi N., Aaron A.D., Clarke R.
LCC15-MB: a vimentin-positive human breast cancer cell line from a femoral bone metastasis.
Clin. Exp. Metastasis 17:193-204(1999)

PubMed=15679051; DOI=10.1007/s10585-004-4117-z
Thompson E.W., Waltham M.C., Ramus S.J., Hutchins A.-M., Armes J.E., Campbell I.G., Williams E.D., Thompson P.R., Rae J.M., Johnson M.D., Clarke R.
LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines.
Clin. Exp. Metastasis 21:535-541(2004)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cell line databases/resources cancercelllines; CVCL_B055
Biological sample resources BioSample; SAMN03151830
Encyclopedic resources Wikidata; Q54904634
Entry history
Entry creation06-Jun-2012
Last entry update05-Oct-2023
Version number26