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Cellosaurus HeLa-Mitotrap (CVCL_AR66)

[Text version]
Cell line name HeLa-Mitotrap
Accession CVCL_AR66
Resource Identification Initiative To cite this cell line use: HeLa-Mitotrap (RRID:CVCL_AR66)
Comments Population: African American.
Characteristics: Can be used to determine the function of a protein by rapidly inactivating the protein of interest by rapamycin-induced rerouting to the mitochondria. This process is termed 'knocksideway'.
Characteristics: Transfected with Mitotrap, a construct composed of the import signal of yeast TOM70, a YFP and the rapamycin-binding (FRB) domain from MTOR.
Transfected with: HGNC; 3942; MTOR (with Met1_Arg2018del and Gln2114_Trp2549del = FRB).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_R965 (HeLa M)
CVCL_AR67 (HeLa-Mitotrap-Ap1g1-FKBP)CVCL_AR69 (HeLa-Mitotrap-Ap2a2-FKBP)CVCL_AR70 (HeLa-Mitotrap-GGA2-FKBP)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Web pages http://www.enterprise.cam.ac.uk/reagents/hela-mitotrap-cell-lines/

PubMed=20159602; DOI=10.1016/j.devcel.2009.12.015
Robinson M.S., Sahlender D.A., Foster S.D.
Rapid inactivation of proteins by rapamycin-induced rerouting to mitochondria.
Dev. Cell 18:324-331(2010)

Cell line collections (Providers) ECACC; 15042201
Ximbio; 152426
Encyclopedic resources Wikidata; Q54882721
Entry history
Entry creation02-May-2016
Last entry update02-May-2024
Version number12