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Cellosaurus N1380 (CVCL_A8RU)

[Text version]
Cell line name N1380
Accession CVCL_A8RU
Resource Identification Initiative To cite this cell line use: N1380 (RRID:CVCL_A8RU)
Comments Population: African; Shangaan.
Virology: Not susceptible to infection by porcine sapelovirus. Transduced with Cas9 and then with the GeCKO v2 whole genome CRISPR/Cas9 library of guide RNAs. The resulting cells were then infected with porcine sapelovirus (PSV) (PubMed=32496007).
Karyotypic information: Has lost chromosome Y (from parent cell line).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0485 (PLC/PRF/5)
Sex of cell Male
Age at sampling 24Y
Category Cancer cell line
Publications

PubMed=32496007; DOI=10.1111/tbed.13655
Wen-Jing Zhang, Michiyo Kataoka, Hai Yen Doan, Fang-Tzy Wu, Kei Haga, Naokazu Takeda, Masamichi Muramatsu, Tian-Cheng Li;
Isolation and characterization of mammalian orthoreoviruses using a cell line resistant to sapelovirus infection.
Transbound. Emerg. Dis. 67:2849-2859(2020)

PubMed=33952773; DOI=10.7883/yoken.JJID.2021.100
Wen-Jing Zhang, Michiyo Kataoka, Hai Yen Doan, Fang-Tzy Wu, Naokazu Takeda, Masamichi Muramatsu, Tian-Cheng Li;
Isolation and characterization of hepatitis E virus subtype 4b using a PLC/PRF/5 cell-derived cell line resistant to porcine sapelovirus infection.
Jpn. J. Infect. Dis. 74:573-575(2021)

Cross-references
Cell line databases/resources cancercelllines; CVCL_A8RU
Encyclopedic resources Wikidata; Q108821004
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number10