ID   N1380
AC   CVCL_A8RU
DR   cancercelllines; CVCL_A8RU
DR   Wikidata; Q108821004
RX   PubMed=32496007;
RX   PubMed=33952773;
CC   Population: African; Shangaan.
CC   Virology: Not susceptible to infection by porcine sapelovirus. Transduced with Cas9 and then with the GeCKO v2 whole genome CRISPR/Cas9 library of guide RNAs. The resulting cells were then infected with porcine sapelovirus (PSV) (PubMed=32496007).
CC   Karyotypic information: Has lost chromosome Y (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P33967; B.cereus Bsr.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C7956; Adult hepatocellular carcinoma
DI   ORDO; Orphanet_210159; Adult hepatocellular carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0485 ! PLC/PRF/5
SX   Male
AG   24Y
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 05-10-23; Version: 6
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RX   PubMed=32496007; DOI=10.1111/tbed.13655;
RA   Zhang W.-J., Kataoka M., Doan H.Y., Wu F.-T., Haga K., Takeda N.,
RA   Muramatsu M., Li T.-C.;
RT   "Isolation and characterization of mammalian orthoreoviruses using a
RT   cell line resistant to sapelovirus infection.";
RL   Transbound. Emerg. Dis. 67:2849-2859(2020).
//
RX   PubMed=33952773; DOI=10.7883/yoken.JJID.2021.100;
RA   Zhang W.-J., Kataoka M., Doan H.Y., Wu F.-T., Takeda N., Muramatsu M.,
RA   Li T.-C.;
RT   "Isolation and characterization of a subtype 4b of hepatitis E virus
RT   using a PLC/PRF/5 cell-derived cell line resistant to porcine
RT   sapelovirus infection.";
RL   Jpn. J. Infect. Dis. 74:573-575(2021).
//