Cellosaurus logo
expasy logo

Cellosaurus IEC6-Cdx2L2 (CVCL_A5RI)

[Text version]
Cell line name IEC6-Cdx2L2
Accession CVCL_A5RI
Resource Identification Initiative To cite this cell line use: IEC6-Cdx2L2 (RRID:CVCL_A5RI)
Comments Characteristics: Cdx2 can conditionally be expressed by addition of IPTG in the culture media. This cell line was engineered with the LacSwitch system in this case composed of two plasmids, pOPRSVI-Cdx2 that contains a RSV LTR promoter, 2 lacO sites (lactose repressor), cdX2 and a neo gene and p3'SS that contains the LacI and hph genes. Thus when IPTG is added to the culture media, repression mediated by lacI is removed and Cdx2 is expressed (PubMed=8552090).
Transfected with: MGI; MGI:88361; Cdx2.
Transfected with: UniProtKB; P03023; Escherichia coli LacI.
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Small intestine; UBERON=UBERON_0002108.
Cell type: Epithelial cell of intestine; CL=CL_0002563.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Charles River Sprague Dawley (CD(SD)).
Hierarchy Parent: CVCL_0343 (IEC-6)
Sex of cell Male
Age at sampling 18-24D
Category Finite cell line

PubMed=8552090; DOI=10.1128/mcb.16.2.619
Suh E., Traber P.G.
An intestine-specific homeobox gene regulates proliferation and differentiation.
Mol. Cell. Biol. 16:619-625(1996)

Encyclopedic resources Wikidata; Q107115431
Entry history
Entry creation20-May-2021
Last entry update05-Oct-2023
Version number8