ID   IEC6-Cdx2L2
AC   CVCL_A5RI
DR   Wikidata; Q107115431
RX   PubMed=8552090;
CC   Characteristics: Cdx2 can conditionally be expressed by addition of IPTG in the culture media. This cell line was engineered with the LacSwitch system in this case composed of two plasmids, pOPRSVI-Cdx2 that contains a RSV LTR promoter, 2 lacO sites (lactose repressor), cdX2 and a neo gene and p3'SS that contains the LacI and hph genes. Thus when IPTG is added to the culture media, repression mediated by lacI is removed and Cdx2 is expressed (PubMed=8552090).
CC   Transfected with: MGI; MGI:88361; Cdx2.
CC   Transfected with: UniProtKB; P03023; Escherichia coli LacI.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Small intestine; UBERON=UBERON_0002108.
CC   Cell type: Epithelial cell of intestine; CL=CL_0002563.
CC   Breed/subspecies: Charles River Sprague Dawley (CD(SD)).
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_0343 ! IEC-6
SX   Male
AG   18-24D
CA   Finite cell line
DT   Created: 20-05-21; Last updated: 05-10-23; Version: 8
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RX   PubMed=8552090; DOI=10.1128/mcb.16.2.619;
RA   Suh E., Traber P.G.;
RT   "An intestine-specific homeobox gene regulates proliferation and
RT   differentiation.";
RL   Mol. Cell. Biol. 16:619-625(1996).
//