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Cellosaurus H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (CVCL_A1HB)

[Text version]
Cell line name H1 EFNB2-tdTomato/EPHB4-EGFP clone 29
Synonyms EFNB2-tdTomato/EPHB4-EGFP dual reporter
Accession CVCL_A1HB
Resource Identification Initiative To cite this cell line use: H1 EFNB2-tdTomato/EPHB4-EGFP clone 29 (RRID:CVCL_A1HB)
Comments Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (from parent cell line).
Characteristics: Using CRISPR/Cas9 a 2A-eGFP-PuroR cassette was integrated in front of the stop codon of one allele of EPHB4 (PubMed=28696312).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A1HA (H1 EFNB2-tdTomato clone 14)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=28696312; DOI=10.1073/pnas.1702295114
Zhang J., Chu L.-F., Hou Z.-G., Schwartz M.P., Hacker T., Vickerman V., Swanson S., Leng N., Nguyen B.K., Elwell A., Bolin J.M., Brown M.E., Stewart R., Burlingham W.J., Murphy W.L., Thomson J.A.
Functional characterization of human pluripotent stem cell-derived arterial endothelial cells.
Proc. Natl. Acad. Sci. U.S.A. 114:E6072-E6078(2017)

Cross-references
Cell line collections (Providers) WiCell; efnb2-tdtomato-ephb4-egfp
Entry history
Entry creation30-Jan-2024
Last entry update30-Jan-2024
Version number1