ID   H1 EFNB2-tdTomato/EPHB4-EGFP clone 29
AC   CVCL_A1HB
SY   EFNB2-tdTomato/EPHB4-EGFP dual reporter
DR   WiCell; efnb2-tdtomato-ephb4-egfp
DR   Wikidata; Q127380968
RX   PubMed=28696312;
CC   Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (from parent cell line).
CC   Characteristics: Using CRISPR/Cas9 a 2A-eGFP-PuroR cassette was integrated in front of the stop codon of one allele of EPHB4 (PubMed=28696312).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A1HA ! H1 EFNB2-tdTomato clone 14
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 3
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RX   PubMed=28696312; DOI=10.1073/pnas.1702295114; PMCID=PMC5544294;
RA   Zhang J., Chu L.-F., Hou Z.-G., Schwartz M.P., Hacker T., Vickerman V.,
RA   Swanson S., Leng N., Nguyen B.K., Elwell A., Bolin J.M., Brown M.E.,
RA   Stewart R., Burlingham W.J., Murphy W.L., Thomson J.A.;
RT   "Functional characterization of human pluripotent stem cell-derived
RT   arterial endothelial cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 114:E6072-E6078(2017).
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