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Cellosaurus H1 EFNB2-tdTomato clone 14 (CVCL_A1HA)

[Text version]
Cell line name H1 EFNB2-tdTomato clone 14
Accession CVCL_A1HA
Resource Identification Initiative To cite this cell line use: H1 EFNB2-tdTomato clone 14 (RRID:CVCL_A1HA)
Comments Characteristics: Using CRISPR/Cas9 a tdTomato-Neo cassette was integrated in front of the stop codon of one allele of EFNB2 (PubMed=28696312).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Children:
CVCL_A1HB (H1 EFNB2-tdTomato/EPHB4-EGFP clone 29)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=28696312; DOI=10.1073/pnas.1702295114; PMCID=PMC5544294
Jue Zhang, Li-Fang Chu, Zhong-Gang Hou, Michael P. Schwartz, Timothy Hacker, Vernella Vickerman, Scott Swanson, Ning Leng, Bao Kim Nguyen, Angela Elwell, Jennifer M. Bolin ...Show all 16 authors... , Matthew E. Brown, Ron Stewart, William J. Burlingham, William L. Murphy, James Alexander Thomson; Show fewer authors
Functional characterization of human pluripotent stem cell-derived arterial endothelial cells.
Proc. Natl. Acad. Sci. U.S.A. 114:E6072-E6078(2017)

Cross-references
Encyclopedic resources Wikidata; Q127380967
Entry history
Entry creation30-Jan-2024
Last entry update14-Aug-2025
Version number3