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Cellosaurus MDA-MB-231-5a-D-TbetaFluc/Rluc-ZsGreen (CVCL_9U33)

[Text version]
Cell line name MDA-MB-231-5a-D-TbetaFluc/Rluc-ZsGreen
Synonyms 5a-D-Luc-ZsGreen
Accession CVCL_9U33
Resource Identification Initiative To cite this cell line use: MDA-MB-231-5a-D-TbetaFluc/Rluc-ZsGreen (RRID:CVCL_9U33)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=FPbase; 51H85; ZsGreen1 (Note=Green fluorescent protein derived from ZsGreen).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9U32 (MDA-MB-231-5a-D-TbetaFluc/Rluc)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Publications

PubMed=26691676; DOI=10.3892/ijo.2015.3300
Hitomi Sudo, Atsushi B. Tsuji, Aya Sugyo, Hiroyuki Takuwa, Kazuto Masamoto, Yutaka Tomita, Norihiro Suzuki, Takeshi Imamura, Mitsuru Koizumi, Tsuneo Saga;
Establishment and evaluation of a new highly metastatic tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and green fluorescent protein.
Int. J. Oncol. 48:525-532(2016)

Cross-references
Cell line databases/resources cancercelllines; CVCL_9U33
Encyclopedic resources Wikidata; Q54904595
Entry history
Entry creation23-Feb-2016
Last entry update14-Aug-2025
Version number20