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Cellosaurus SAML-1 (CVCL_9997)

[Text version]
Cell line name SAML-1
Accession CVCL_9997
Resource Identification Initiative To cite this cell line use: SAML-1 (RRID:CVCL_9997)
Comments Problematic cell line: Contaminated. Shown to be a U-937 derivative. Originally thought to originate from a bone marrow aspirate from a 50 year old male patient suffering from a secondary acute myelogenous leukemia (AML) following Hodgkin's disease.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00424.
Population: Caucasian.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:16063; MLLT10 + HGNC; HGNC:15514; PICALM; Name(s)=PICALM-MLLT10, CALM-AF10 (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Gly129fs*51 (c.387_388insCGCC); Zygosity=Hemizygous (from parent cell line).
  • Mutation; HGNC; HGNC:9644; PTPN11; Simple; p.Gly60Arg (c.178G>C); ClinVar=VCV000372590; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; c.559+1G>A (p.Val173Trpfs*59); ClinVar=VCV000428908; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:12796; WT1; Simple; p.Arg301Ter (c.901C>T) (p.Arg352Ter, c.1054C>T) (p.Arg374Ter, c.1120C>T); ClinVar=VCV000449416; Zygosity=Heterozygous (from parent cell line).
Disease Adult acute monocytic leukemia (NCIt: C8263)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0007 (U-937)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2776

Markers:
AmelogeninX
CSF1PO12
D5S81812
D7S8209,11
D13S31710,12
D16S53912
TH016,9.3
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/saml-1-atcc-crl-2776
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=12759925; DOI=10.1002/gcc.10200
Turid Knutsen, Svetlana Pack, Maria Petropavlovskaja, Hesed Maria Padilla-Nash, Clement Knight, Lyn A. Mickley, Thomas Ried, Patrick C. Elwood, Susan J. Roberts;
Cytogenetic, spectral karyotyping, fluorescence in situ hybridization, and comparative genomic hybridization characterization of two new secondary leukemia cell lines with 5q deletions, and MYC and MLL amplification.
Genes Chromosomes Cancer 37:270-281(2003)

PubMed=29533902; DOI=10.1515/hsz-2017-0321
Hans Gunther Drexler, Claudia Pommerenke, Sonja Eberth, Stefan Nagel;
Hodgkin lymphoma cell lines: to separate the wheat from the chaff.
Biol. Chem. 399:511-523(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-2776 - Discontinued
Cell line databases/resources cancercelllines; CVCL_9997
SKY/M-FISH/CGH; 1472
Biological sample resources BioSample; SAMN03151985
Encyclopedic resources Wikidata; Q54952088
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number29