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Cellosaurus Hu456 (CVCL_8238)

[Text version]
Cell line name Hu456
Synonyms Hu 456; HU 456; HU456
Accession CVCL_8238
Resource Identification Initiative To cite this cell line use: Hu456 (RRID:CVCL_8238)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (PubMed=3708594; PubMed=8105864; PubMed=20143388).
Part of: BLA-40 bladder carcinoma cell line panel.
Part of: UBC-40 urothelial bladder cancer cell line index.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00129.
Population: Caucasian; Swedish.
Doubling time: 21 hours (PubMed=3708594).
Omics: Transcriptomics; Microarray.
Omics: Variations; CNV analysis.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): PubMed=11416159

Markers:
AmelogeninX
D8S117914
D18S5116,18
D21S1129
FGA22
TH016
vWA17

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=7262169; DOI=10.1016/0014-2964(81)90123-7
Mogens Vilien, Hans Wolf, Finn Rasmussen;
Immunological characterization of cell lines establishing from malignant and normal human urothelium.
Eur. J. Cancer 17:321-327(1981)

PubMed=6546195; DOI=10.1007/BF00121494
Wilfried Schroyens, Marc M. Mareel, Christian H. Dragonetti;
In vitro invasiveness of human bladder cancer from cell lines and biopsy specimens.
Clin. Exp. Metastasis 1:153-162(1983)

PubMed=6823318; DOI=10.1038/301429a0
Carol M. O'Toole, Susan Povey, Peter J. Hepburn, Leonard Maurice Franks;
Identity of some human bladder cancer cell lines.
Nature 301:429-430(1983)

PubMed=6826254; DOI=10.1002/ijc.2910310308
Staffan Paulie, Yngve Hansson, Marie-Louise Lundblad, Peter Perlmann;
Lectins as probes for identification of tumor-associated antigens on urothelial and colonic carcinoma cell lines.
Int. J. Cancer 31:297-303(1983)

PubMed=3708594
John R.W. Masters, Peter J. Hepburn, Lawrence Walker, Wilma J. Highman, Ludwik K. Trejdosiewicz, Susan Povey, Mohamed Parkar, Bridget T. Hill, Peter N. Riddle, Leonard Maurice Franks;
Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.
Cancer Res. 46:3630-3636(1986)

PubMed=8105864; DOI=10.1038/bjc.1993.449; PMCID=PMC1968741
Britta Christensen, Claus Hansen, Maria Debiec-Rychter, Jorgen Kieler, S. Ottensen, Jacqueline Schmidt;
Identity of tumorigenic human urothelial cell lines and 'spontaneously' transformed sublines.
Br. J. Cancer 68:879-884(1993)

PubMed=8900435; DOI=10.1002/(SICI)1097-0215(19961009)68:2<239::AID-IJC17>3.0.CO;2-5
Arkadiusz Grzegorz Klopocki, Anna Krop-Watorek, Danuta Dus, Maciej Ugorski;
Adhesion of human uroepithelial cells to E-selectin: possible involvement of sialosyl LewisA-ganglioside.
Int. J. Cancer 68:239-244(1996)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=24018021; DOI=10.1016/j.eururo.2013.08.052
Yves Allory, Willemien Beukers, Ana Sagrera, Marta Flandez, Miriam Marques, Mirari Marquez, Kirstin A. van der Keur, Lars Dyrskjot, Irene Lurkin, Marcel Vermeij, Alfredo Carrato ...Show all 24 authors... , Josep Lloreta, Jose A. Lorente, Enrique Carrillo-de-Santa-Pau, Roy G. Masius, Manolis Kogevinas, Ewout Willem Steyerberg, Angela A.G. van Tilborg, Cheno Abas, Torben Falck Orntoft, Tahlita C.M. Zuiverloon, Nuria Malats, Ellen C. Zwarthoff, Francisco X. Real; Show fewer authors
Telomerase reverse transcriptase promoter mutations in bladder cancer: high frequency across stages, detection in urine, and lack of association with outcome.
Eur. Urol. 65:360-366(2014)

PubMed=25997541; DOI=10.1186/s12864-015-1450-3; PMCID=PMC4470036
Julie Earl, Daniel Rico, Enrique Carrillo-de-Santa-Pau, Benjamin Rodriguez-Santiago, Marinela Mendez-Pertuz, Herbert Auer, Gonzalo Gomez, Herbert Barton Grossman, David G. Pisano, Wolfgang A. Schulz, Luis A. Perez-Jurado ...Show all 16 authors... , Alfredo Carrato, Dan Theodorescu, Stephen Jacob Chanock, Alfonso Valencia, Francisco X. Real; Show fewer authors
The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies.
BMC Genomics 16:403.1-403.16(2015)

Cross-references
Cell line databases/resources cancercelllines; CVCL_8238
Biological sample resources BioSample; SAMN03151943
Encyclopedic resources Wikidata; Q54896699
Gene expression databases GEO; GSM136253
GEO; GSM1574544
Polymorphism and mutation databases Cosmic; 925829
Cosmic; 2037947
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number31