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Cellosaurus HT-29-D4 (CVCL_8118)

[Text version]
Cell line name HT-29-D4
Synonyms HT 29-D4; HT29-D4
Accession CVCL_8118
Resource Identification Initiative To cite this cell line use: HT-29-D4 (RRID:CVCL_8118)
Comments Population: Caucasian.
Characteristics: Can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (PubMed=3543033).
Doubling time: 48 hours (Note=With glucose), 60 hours (Note=With galactose) (PubMed=3543033).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
Publications

PubMed=3543033; DOI=10.1242/jcs.83.1.235
Fantini J., Abadie B., Tirard A., Remy L., Ripert J.P., el Battari A., Marvaldi J.
Spontaneous and induced dome formation by two clonal cell populations derived from a human adenocarcinoma cell line, HT29.
J. Cell Sci. 83:235-249(1986)

PubMed=2736330; DOI=10.1111/j.1768-322X.1989.tb00785.x
Fantini J., Verrier B., Marvaldi J., Mauchamp J.
In vitro differentiated HT 29-D4 clonal cell line generates leakproof and electrically active monolayers when cultured in porous-bottom culture dishes.
Biol. Cell 65:163-169(1989)

Cross-references
Cell line databases/resources cancercelllines; CVCL_8118
Lonza; 371
Encyclopedic resources Wikidata; Q54896512
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number23