ID   HT-29-D4
AC   CVCL_8118
SY   HT 29-D4; HT29-D4
DR   cancercelllines; CVCL_8118
DR   Lonza; 371
DR   Wikidata; Q54896512
RX   PubMed=2736330;
RX   PubMed=3543033;
CC   Population: Caucasian.
CC   Characteristics: Can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (PubMed=3543033).
CC   Doubling time: 48 hours (Note=With glucose), 60 hours (Note=With galactose) (PubMed=3543033).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0320 ! HT-29
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 23
//
RX   PubMed=2736330; DOI=10.1111/j.1768-322X.1989.tb00785.x;
RA   Fantini J., Verrier B., Marvaldi J., Mauchamp J.;
RT   "In vitro differentiated HT 29-D4 clonal cell line generates leakproof
RT   and electrically active monolayers when cultured in porous-bottom
RT   culture dishes.";
RL   Biol. Cell 65:163-169(1989).
//
RX   PubMed=3543033; DOI=10.1242/jcs.83.1.235;
RA   Fantini J., Abadie B., Tirard A., Remy L., Ripert J.P., el Battari A.,
RA   Marvaldi J.;
RT   "Spontaneous and induced dome formation by two clonal cell populations
RT   derived from a human adenocarcinoma cell line, HT29.";
RL   J. Cell Sci. 83:235-249(1986).
//