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Cellosaurus 253J (CVCL_7935)

[Text version]
Cell line name 253J
Synonyms 253j; 253-J; 253 J; 253J-P; 253J-Parental; 253J P; 253JP
Accession CVCL_7935
Secondary accession CVCL_7938
Resource Identification Initiative To cite this cell line use: 253J (RRID:CVCL_7935)
Comments Part of: BLA-40 bladder carcinoma cell line panel.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: UBC-40 urothelial bladder cancer cell line index.
Population: Caucasian.
Doubling time: 36 hours (PubMed=66978; PubMed=748774); 46 +- 3 hours (PubMed=7658585); 28 hours (PubMed=3708594).
Microsatellite instability: Stable (MSS) (PubMed=31068700).
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; CNV analysis.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Retroperitoneal lymph node; UBERON=UBERON_0035371.
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,03:01
HLA-BB*08:01,51:01
HLA-CC*07:01,12:03
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_7936 (253J laval)CVCL_7937 (253J-BV)CVCL_6G34 (253J-Lung-IV)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): KCLB=80001; PubMed=11416159; PubMed=27270441

Markers:
AmelogeninX
CSF1PO10,12
D3S135815
D5S81810,12
D7S8209
D8S117911
D13S3179
D16S5399,12
D18S5111,13
D21S1128,29
FGA23,24
TH016,9.3
TPOX8,11
vWA17,18

Run an STR similarity search on this cell line
Web pages Info; Synapse; Bladder Cancer exome; https://www.synapse.org/Synapse:syn4970200
Publications

PubMed=4431054; DOI=10.1093/jnci/53.5.1341
Arthur Y. Elliott, Patrick H. Cleveland, Jaroslov Cervenka, Anthony E. Castro, Nell Stein, Thomas Richard Hakala, Elwin Eugene Fraley;
Characterization of a cell line from human transitional cell cancer of the urinary tract.
J. Natl. Cancer Inst. 53:1341-1349(1974)

PubMed=944089
Arthur Y. Elliott, David Leigh Bronson, Nell Stein, Elwin Eugene Fraley;
In vitro cultivation of epithelial cells derived from tumors of the human urinary tract.
Cancer Res. 36:365-369(1976)

DOI=10.1016/B978-0-12-043050-5.50049-4
Arthur Y. Elliott, David Leigh Bronson, Jaroslov Cervenka, Patrick H. Cleveland, Elwin Eugene Fraley;
Establishment and characterization of epithelial cell cultures from tumors of the human urinary tract.
(In book chapter) Cell culture and its application; Acton, Ronald T. (eds.); pp.711-728; Academic Press; New York; USA (1977)

PubMed=66978
Arthur Y. Elliott, David Leigh Bronson, Jaroslov Cervenka, Nell Stein, Elwin Eugene Fraley;
Properties of cell lines established from transitional cell cancers of the human urinary tract.
Cancer Res. 37:1279-1289(1977)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=571047
Jorgen Fogh;
Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.
Natl. Cancer Inst. Monogr. 49:5-9(1978)

PubMed=748774
Arthur Y. Elliott, David Leigh Bronson, Elwin Eugene Fraley;
Transitional cell cancer: establishment and characterization of cell lines.
Natl. Cancer Inst. Monogr. 49:23-24(1978)

PubMed=6244232
Richard D. Williams;
Human urologic cancer cell lines.
Invest. Urol. 17:359-363(1980)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
William C. Wright, Walter P. Daniels, Jorgen Fogh;
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Monique Rousset, Alain Zweibaum, Jorgen Fogh;
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720
Michael Jules Mattes, Carlos Cordon-Cardo, John L. Lewis Jr., Lloyd John Old, Kenneth Oliver Lloyd;
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3708594
John R.W. Masters, Peter J. Hepburn, Lawrence Walker, Wilma J. Highman, Ludwik K. Trejdosiewicz, Susan Povey, Mohamed Parkar, Bridget T. Hill, Peter N. Riddle, Leonard Maurice Franks;
Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.
Cancer Res. 46:3630-3636(1986)

PubMed=7658585; DOI=10.1016/S0022-5347(01)66923-4
Colin Padraic Neal Dinney, Randi Fishbeck, Rakesh K. Singh, Beryl Eve, Sen Pathak, Nicolas Brown, Bei Xie, Dominic Fan, Corazon D. Bucana, Isaiah Joshua Fidler, Jerald J. Killion;
Isolation and characterization of metastatic variants from human transitional cell carcinoma passaged by orthotopic implantation in athymic nude mice.
J. Urol. 154:1532-1538(1995)

PubMed=8873383; DOI=10.1007/BF00295899
Walter M. Stadler, Olufunmilayo I. Olopade;
The 9p21 region in bladder cancer cell lines: large homozygous deletion inactivate the CDKN2, CDKN2B and MTAP genes.
Urol. Res. 24:239-244(1996)

PubMed=11207054; DOI=10.1054/bjoc.2000.1641; PMCID=PMC2363759
Chisato Fujiyama, Adam Jones, Susan Valerie Fuggle, Roy Bicknell, David Cranston, Adrian L. Harris;
Human bladder cancer invasion model using rat bladder in vitro and its use to test mechanisms and therapeutic inhibitors of invasion.
Br. J. Cancer 84:558-564(2001)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11921286; DOI=10.1002/gcc.10050
Sarah V. Williams, Kathryn D. Sibley, Alison M. Davies, Hiroyuki Nishiyama, Nick Hornigold, Jane Coulter, Wendy J. Kennedy, Amy Skilleter, Tomonori Habuchi, Margaret A. Knowles;
Molecular genetic analysis of chromosome 9 candidate tumor-suppressor loci in bladder cancer cell lines.
Genes Chromosomes Cancer 34:86-96(2002)

PubMed=12127398; DOI=10.1016/S0165-4608(01)00648-3
Jonathan C. Strefford, Debra M. Lillington, Martin Steggall, Tim M. Lane, Ahmad M.E. Nouri, Bryan D. Young, R. Timothy D. Oliver;
Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization.
Cancer Genet. Cytogenet. 135:139-146(2002)

PubMed=15846775; DOI=10.1002/gcc.20166
Sarah V. Williams, Jacqui Adams, Jane Coulter, Brenda M. Summersgill, Janet M. Shipley, Margaret A. Knowles;
Assessment by M-FISH of karyotypic complexity and cytogenetic evolution in bladder cancer in vitro.
Genes Chromosomes Cancer 43:315-328(2005)

PubMed=16885334; DOI=10.1158/0008-5472.CAN-06-1182
Elena Lopez-Knowles, Silvia Hernandez, Nuria Malats, Manolis Kogevinas, Josep Lloreta, Alfredo Carrato, Adonina Tardon, Consol Serra, Francisco X. Real;
PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors.
Cancer Res. 66:7401-7404(2006)

PubMed=23401075; DOI=10.1002/path.4176
Ya-Nan Guo, Yvonne Chekaluk, Jian-Ming Zhang, Jin-Yan Du, Nathanael S. Gray, Chin-Lee Wu, David Joseph Kwiatkowski;
TSC1 involvement in bladder cancer: diverse effects and therapeutic implications.
J. Pathol. 230:17-27(2013)

PubMed=24367658; DOI=10.1371/journal.pone.0084411; PMCID=PMC3867501
Rebecca L. Ross, Julie E. Burns, Claire F. Taylor, Paul Mellor, Deborah H. Anderson, Margaret A. Knowles;
Identification of mutations in distinct regions of p85 alpha in urothelial cancer.
PLoS ONE 8:e84411.1-e84411.11(2013)

PubMed=24035680; DOI=10.1016/j.eururo.2013.08.057
Carolyn D. Hurst, Fiona M. Platt, Margaret A. Knowles;
Comprehensive mutation analysis of the TERT promoter in bladder cancer and detection of mutations in voided urine.
Eur. Urol. 65:367-369(2014)

PubMed=25997541; DOI=10.1186/s12864-015-1450-3; PMCID=PMC4470036
Julie Earl, Daniel Rico, Enrique Carrillo-de-Santa-Pau, Benjamin Rodriguez-Santiago, Marinela Mendez-Pertuz, Herbert Auer, Gonzalo Gomez, Herbert Barton Grossman, David G. Pisano, Wolfgang A. Schulz, Luis A. Perez-Jurado ...Show all 16 authors... , Alfredo Carrato, Dan Theodorescu, Stephen Jacob Chanock, Alfonso Valencia, Francisco X. Real; Show fewer authors
The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies.
BMC Genomics 16:403.1-403.16(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26621286; DOI=10.1186/s12864-015-2227-4; PMCID=PMC4681452
Julie Earl, Daniel Rico, Enrique Carrillo-de-Santa-Pau, Benjamin Rodriguez-Santiago, Marinela Mendez-Pertuz, Herbert Auer, Gonzalo Gomez, Herbert Barton Grossman, David G. Pisano, Wolfgang A. Schulz, Luis A. Perez-Jurado ...Show all 16 authors... , Alfredo Carrato, Dan Theodorescu, Stephen Jacob Chanock, Alfonso Valencia, Francisco X. Real; Show fewer authors
Erratum to: The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies.
BMC Genomics 16:1019.1-1019.2(2015)

PubMed=27270441; DOI=10.1038/onc.2016.172; PMCID=PMC5140783
Michael L. Nickerson, Nicolle Witte, Kate McGee Im, Sevilay Turan, Charles R. Owens, Kevin Misner, Shirley X. Tsang, Zhi-Ming Cai, Sen Wu, Michael Dean, James C. Costello, Dan Theodorescu;
Molecular analysis of urothelial cancer cell lines for modeling tumor biology and drug response.
Oncogene 36:35-46(2017)

PubMed=29732388; DOI=10.3233/BLC-180167; PMCID=PMC5929350
Tahlita C.M. Zuiverloon, Florus C. de Jong, James C. Costello, Dan Theodorescu;
Systematic review: characteristics and preclinical uses of bladder cancer cell lines.
Bladder Cancer 4:169-183(2018)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) ATCC; HTB-108 - Discontinued
KCLB; 80001
Cell line databases/resources cancercelllines; CVCL_7935
Cell_Model_Passport; SIDM01529
DepMap; ACH-000011
Anatomy/cell type resources BTO; BTO_0004796
Biological sample resources BioSample; SAMN10987961
Chemistry resources PharmacoDB; 253J_14_2019
Encyclopedic resources Wikidata; Q54583653
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSM136240
GEO; GSM1574546
GEO; GSM1574574
Polymorphism and mutation databases Cosmic; 715794
Cosmic; 760476
Cosmic; 845564
Cosmic; 925827
Cosmic; 928820
Cosmic; 1016884
Cosmic; 1046680
Cosmic; 1082644
Cosmic; 1285102
Cosmic; 1285997
Cosmic; 1927305
Cosmic; 1938694
Cosmic; 2050426
Cosmic; 2057433
Cosmic; 2444255
Cosmic; 2685929
Cosmic; 2700994
IARC_TP53; 21034
LiGeA; CCLE_259
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number32