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Cellosaurus WTK-1 (CVCL_6742)

[Text version]
Cell line name WTK-1
Synonyms WTK1
Accession CVCL_6742
Resource Identification Initiative To cite this cell line use: WTK-1 (RRID:CVCL_6742)
Comments Group: Space-flown cell line (cellonaut).
Population: Caucasian.
Doubling time: 12.7 hours (PubMed=7744731).
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
Omics: Transcriptome analysis by RNAseq.
Anecdotal: Have been flown in space on the ISS in 2008-2009 to study the biological effects of space radiations, microgravity and the space environment on the expression of TP53-regulated genes (PubMed=20673130).
Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
Sequence variations
  • Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=11221843).
  • Mutation; HGNC; 11998; TP53; Simple; p.Met237Ile (c.711G>A); ClinVar=VCV000142714; Zygosity=Homozygous (PubMed=7744731; PubMed=11221843; PubMed=22525470).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2761 (WIL2 NS)
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line

PubMed=7744731; DOI=10.1074/jbc.270.19.11033
Little J.B., Nagasawa H., Keng P.C., Yu Y., Li C.-Y.
Absence of radiation-induced G1 arrest in two closely related human lymphoblast cell lines that differ in p53 status.
J. Biol. Chem. 270:11033-11036(1995)

Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.
Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.
Cancer Res. 59:3073-3076(1999)

Wiese C., Gauny S.S., Liu W.-C., Cherbonnel-Lasserre C.L., Kronenberg A.
Different mechanisms of radiation-induced loss of heterozygosity in two human lymphoid cell lines from a single donor.
Cancer Res. 61:1129-1137(2001)

PubMed=11566342; DOI=10.1016/S0165-4608(01)00440-X
Schwartz J.L., Jordan R., Evans H.H.
Characteristics of chromosome instability in the human lymphoblast cell line WTK1.
Cancer Genet. Cytogenet. 129:124-130(2001)

PubMed=20673130; DOI=10.3109/09553001003789596
Takahashi A., Suzuki H., Omori K., Seki M., Hashizume T., Shimazu T., Ishioka N., Ohnishi T.
The expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines during spaceflight.
Int. J. Radiat. Biol. 86:669-681(2010)

PubMed=22525470; DOI=10.18926/AMO/48262
Oka H., Ouchida M., Kondo T., Morita F., Shimizu K.
Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation.
Acta Med. Okayama 66:119-129(2012)

PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006
Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B., Dobrovolsky V.N.
Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.
Mutat. Res. 795:60-69(2016)

Anatomy/cell type resources BTO; BTO:0003706
Encyclopedic resources Wikidata; Q54994556
Polymorphism and mutation databases IARC_TP53; 15513
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number17