ID   WTK-1
AC   CVCL_6742
SY   WTK1
DR   BTO; BTO:0003706
DR   IARC_TP53; 15513
DR   Wikidata; Q54994556
RX   PubMed=7744731;
RX   PubMed=10397247;
RX   PubMed=11221843;
RX   PubMed=11566342;
RX   PubMed=20673130;
RX   PubMed=22525470;
RX   PubMed=26774668;
CC   Group: Space-flown cell line (cellonaut).
CC   Population: Caucasian.
CC   Doubling time: 12.7 hours (PubMed=7744731).
CC   Sequence variation: Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=11221843).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Met237Ile (c.711G>A); ClinVar=VCV000142714; Zygosity=Homozygous (PubMed=7744731; PubMed=11221843; PubMed=22525470).
CC   Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
CC   Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
CC   Omics: Transcriptome analysis by RNAseq.
CC   Anecdotal: Have been flown in space on the ISS in 2008-2009 to study the biological effects of space radiations, microgravity and the space environment on the expression of TP53-regulated genes (PubMed=20673130).
CC   Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
DI   NCIt; C97074; Hereditary spherocytosis
DI   ORDO; Orphanet_822; Hereditary spherocytosis
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2761 ! WIL2 NS
SX   Male
AG   5Y
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 17
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RX   PubMed=7744731; DOI=10.1074/jbc.270.19.11033;
RA   Little J.B., Nagasawa H., Keng P.C., Yu Y.-J., Li C.-Y.;
RT   "Absence of radiation-induced G1 arrest in two closely related human
RT   lymphoblast cell lines that differ in p53 status.";
RL   J. Biol. Chem. 270:11033-11036(1995).
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RX   PubMed=10397247;
RA   Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.;
RT   "Radiation-induced mutations at the autosomal thymidine kinase locus
RT   are not elevated in p53-null cells.";
RL   Cancer Res. 59:3073-3076(1999).
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RX   PubMed=11221843;
RA   Wiese C., Gauny S.S., Liu W.-C., Cherbonnel-Lasserre C.L.,
RA   Kronenberg A.;
RT   "Different mechanisms of radiation-induced loss of heterozygosity in
RT   two human lymphoid cell lines from a single donor.";
RL   Cancer Res. 61:1129-1137(2001).
//
RX   PubMed=11566342; DOI=10.1016/S0165-4608(01)00440-X;
RA   Schwartz J.L., Jordan R., Evans H.H.;
RT   "Characteristics of chromosome instability in the human lymphoblast
RT   cell line WTK1.";
RL   Cancer Genet. Cytogenet. 129:124-130(2001).
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RX   PubMed=20673130; DOI=10.3109/09553001003789596;
RA   Takahashi A., Suzuki H., Omori K., Seki M., Hashizume T., Shimazu T.,
RA   Ishioka N., Ohnishi T.;
RT   "The expression of p53-regulated genes in human cultured
RT   lymphoblastoid TSCE5 and WTK1 cell lines during spaceflight.";
RL   Int. J. Radiat. Biol. 86:669-681(2010).
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RX   PubMed=22525470; DOI=10.18926/AMO/48262;
RA   Oka H., Ouchida M., Kondo T., Morita F., Shimizu K.;
RT   "Different responses to 5-fluoraouracil in mutagenicity and gene
RT   expression between two human lymphoblastoid cell lines with or without
RT   TP53 mutation.";
RL   Acta Med. Okayama 66:119-129(2012).
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RX   PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006;
RA   Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B.,
RA   Dobrovolsky V.N.;
RT   "Whole genome and normalized mRNA sequencing reveal genetic status of
RT   TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.";
RL   Mutat. Res. 795:60-69(2016).
//