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Cellosaurus BJ1-hTERT (CVCL_6573)

[Text version]
Cell line name BJ1-hTERT
Synonyms hTERT-BJ1; HTERT-BJ1; hTERT-BJ; hTERT BJ; BJ hTERT; BJ1; BJ-1; BJ-5ta; BJ-tert; BJhTERT; BJHTERT
Accession CVCL_6573
Secondary accession CVCL_3654
Resource Identification Initiative To cite this cell line use: BJ1-hTERT (RRID:CVCL_6573)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Doubling time: 36 hours (PubMed=25984343).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.29
Native American0.6
East Asian, North1.01
East Asian, South0
South Asian0.05
European, North73.33
European, South24.72
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3653 (BJ [Human fibroblast])
Sex of cell Male
Age at sampling <1M
Category Telomerase immortalized cell line
STR profile Source(s): ATCC=CRL-4001; PubMed=25877200; Technion Genomics Center

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165612,16
D2S44111
D2S133816,20
D3S135814,16
D5S81812
D7S82011,12
D8S11799,11
D10S124813,17
D12S39115,23
D13S3178,9
D16S5399,13
D18S5117,19
D19S43314
D21S1129
D22S104511,15
DYS39110
FGA22,23
Penta D12,13
Penta E7,17
TH017,8
TPOX10,11
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/number-of-population-doublings-pdl-for-atcc-crl-4001
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/BJ-5ta_Struhl_protocol.pdf
Publications

PubMed=9454332; DOI=10.1126/science.279.5349.349
Andrea G. Bodnar, Michel M. Ouellette, Maria Frolkis, Shawn E. Holt, Choy-Pik Chiu, Gregg B. Morin, Calvin B. Harley, Jerry W. Shay, Serge Patrick Lichtsteiner, Woodring Erik Wright;
Extension of life-span by introduction of telomerase into normal human cells.
Science 279:349-352(1998)

PubMed=10655550; DOI=10.1093/hmg/9.3.403
Michel M. Ouellette, Lisa D. McDaniel, Woodring Erik Wright, Jerry W. Shay, Roger A. Schultz;
The establishment of telomerase-immortalized cell lines representing human chromosome instability syndromes.
Hum. Mol. Genet. 9:403-411(2000)

PubMed=24119401; DOI=10.1016/j.molcel.2013.09.009; PMCID=PMC4039290
Jia-Yun Chen, Jia-Ren Lin, Feng-Chiao Tsai, Tobias Meyer;
Dosage of Dyrk1a shifts cells within a p21-cyclin D1 signaling map to control the decision to enter the cell cycle.
Mol. Cell 52:87-100(2013)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) ATCC; CRL-4001
Cell line databases/resources CLO; CLO_0001981
cancercelllines; CVCL_6573
Cell_Model_Passport; SIDM01927
DepMap; ACH-001018
Biological sample resources BioSample; SAMN03472773
Chemistry resources ChEMBL-Cells; CHEMBL4295443
ChEMBL-Targets; CHEMBL4296512
PharmacoDB; BJhTERT_101_2019
PubChem_Cell_line; CVCL_6573
Encyclopedic resources Wikidata; Q54796970
Gene expression databases GEO; GSM7701460
GEO; GSM7701464
Polymorphism and mutation databases Progenetix; CVCL_6573
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number35