ID   BJ1-hTERT
AC   CVCL_6573
AS   CVCL_3654
SY   hTERT-BJ1; HTERT-BJ1; hTERT-BJ; hTERT BJ; BJ hTERT; BJ1; BJ-1; BJ-5ta; BJ-tert; BJhTERT; BJHTERT
DR   CLO; CLO_0001981
DR   ATCC; CRL-4001
DR   BioSample; SAMN03472773
DR   cancercelllines; CVCL_6573
DR   Cell_Model_Passport; SIDM01927
DR   ChEMBL-Cells; CHEMBL4295443
DR   ChEMBL-Targets; CHEMBL4296512
DR   DepMap; ACH-001018
DR   PharmacoDB; BJhTERT_101_2019
DR   Progenetix; CVCL_6573
DR   PubChem_Cell_line; CVCL_6573
DR   Wikidata; Q54796970
RX   PubMed=9454332;
RX   PubMed=10655550;
RX   PubMed=24119401;
RX   PubMed=25877200;
RX   PubMed=25984343;
RX   PubMed=30894373;
RX   PubMed=31068700;
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/BJ-5ta_Struhl_protocol.pdf
WW   https://www.atcc.org/en/support/technical-support/faqs/number-of-population-doublings-pdl-for-atcc-crl-4001
WW   http://www.immanis.com/TelomeraseImmortalizedCellLineCulturingGuide.pdf
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Population: Caucasian.
CC   Doubling time: 36 hours (PubMed=25984343).
CC   Genetic integration: Method=Transfection; Gene=HGNC; 11730; TERT.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genome ancestry: African=0.29%; Native American=0.6%; East Asian, North=1.01%; East Asian, South=0%; South Asian=0.05%; European, North=73.33%; European, South=24.72% (PubMed=30894373).
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
ST   Source(s): ATCC=CRL-4001; PubMed=25877200; Technion Genomics Center
ST   Amelogenin: X,Y
ST   CSF1PO: 10,12
ST   D10S1248: 13,17
ST   D12S391: 15,23
ST   D13S317: 8,9
ST   D16S539: 9,13
ST   D18S51: 17,19
ST   D19S433: 14
ST   D1S1656: 12,16
ST   D21S11: 29
ST   D22S1045: 11,15
ST   D2S1338: 16,20
ST   D2S441: 11
ST   D3S1358: 14,16
ST   D5S818: 12
ST   D7S820: 11,12
ST   D8S1179: 9,11
ST   DYS391: 10
ST   FGA: 22,23
ST   Penta D: 12,13
ST   Penta E: 7,17
ST   TH01: 7,8
ST   TPOX: 10,11
ST   vWA: 16,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_3653 ! BJ [Human fibroblast]
SX   Male
AG   <1M
CA   Telomerase immortalized cell line
DT   Created: 04-04-12; Last updated: 10-09-24; Version: 33
//
RX   PubMed=9454332; DOI=10.1126/science.279.5349.349;
RA   Bodnar A.G., Ouellette M.M., Frolkis M., Holt S.E., Chiu C.-P.,
RA   Morin G.B., Harley C.B., Shay J.W., Lichtsteiner S.P., Wright W.E.;
RT   "Extension of life-span by introduction of telomerase into normal
RT   human cells.";
RL   Science 279:349-352(1998).
//
RX   PubMed=10655550; DOI=10.1093/hmg/9.3.403;
RA   Ouellette M.M., McDaniel L.D., Wright W.E., Shay J.W., Schultz R.A.;
RT   "The establishment of telomerase-immortalized cell lines representing
RT   human chromosome instability syndromes.";
RL   Hum. Mol. Genet. 9:403-411(2000).
//
RX   PubMed=24119401; DOI=10.1016/j.molcel.2013.09.009; PMCID=PMC4039290;
RA   Chen J.-Y., Lin J.-R., Tsai F.-C., Meyer T.;
RT   "Dosage of Dyrk1a shifts cells within a p21-cyclin D1 signaling map to
RT   control the decision to enter the cell cycle.";
RL   Mol. Cell 52:87-100(2013).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M.,
RA   Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S.,
RA   Neve R.M.;
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652;
RA   Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S.,
RA   East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H.,
RA   Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M.,
RA   Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S.,
RA   Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D.,
RA   Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C.,
RA   Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P.,
RA   Golub T.R., Root D.E., Hahn W.C.;
RT   "Parallel genome-scale loss of function screens in 216 cancer cell
RT   lines for the identification of context-specific genetic
RT   dependencies.";
RL   Sci. Data 1:140035-140035(2014).
//
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675;
RA   Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.;
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103;
RA   Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C.,
RA   McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X.,
RA   Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F.,
RA   Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R.,
RA   Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C.,
RA   Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A.,
RA   Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D.,
RA   Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L.,
RA   Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A.,
RA   Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D.,
RA   Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M.,
RA   Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R.,
RA   Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A.,
RA   Sellers W.R.;
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//