Cellosaurus cell line BJ [Human fibroblast] (CVCL

Cellosaurus BJ [Human fibroblast] (CVCL_3653)

[Text version]

Cell line name

BJ [Human fibroblast]

Synonyms

FF-WT-BJ; BJ1

Accession

CVCL_3653

Resource Identification Initiative

To cite this cell line use: BJ [Human fibroblast] (RRID:CVCL_3653)

Comments

Part of: ENCODE project common cell types; tier 3.
Part of: Human Protein Atlas, Cell Atlas panel.
Population: Caucasian.
Senescence: Senesces at 72 PDL. Has a long lifespan in comparison with other normal human fibroblast cell lines (ATCC=CRL-2522).
Omics: Genomics; ChIP-seq; CTCF.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K36me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Proteomics; Expression; Large antibody panel staining analysis.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Children:

CVCL_C243 (246G1)	CVCL_C244 (246G3)	CVCL_C245 (246G4)
CVCL_C246 (246G5)	CVCL_C247 (246G6)	CVCL_DQ33 (346D1)
CVCL_DQ34 (346E1)	CVCL_DQ35 (346E2)	CVCL_DQ36 (346F1)
CVCL_DQ37 (363G1)	CVCL_VG15 (4BJ1)	CVCL_VG16 (5B1)
CVCL_VG17 (7B1)	CVCL_VG18 (7B6)	CVCL_C623 (B12-11)
CVCL_C620 (B12-2)	CVCL_C621 (B12-3)	CVCL_C622 (B12-6)
CVCL_C624 (B124-1)	CVCL_C626 (B124-10)	CVCL_C627 (B124-13)
CVCL_C625 (B124-2)	CVCL_A4YN (BGUi015-A)	CVCL_X732 (BJ epi iPS-C)
CVCL_6D12 (BJ hiPSC #11)	CVCL_6D13 (BJ hiPSC #12)	CVCL_6D14 (BJ hiPSC #5)
CVCL_6D15 (BJ hiPSC #8)	CVCL_A4QM (BJ iPSC clone 1)	CVCL_A4QN (BJ iPSC clone 2)
CVCL_A4QP (BJ iPSC clone 3)	CVCL_X737 (BJ RiPS-A)	CVCL_X738 (BJ RiPS-D)
CVCL_X739 (BJ RiPS-E)	CVCL_X740 (BJ SiPS-C)	CVCL_X741 (BJ SiPS-D)
CVCL_C922 (BJ-RiPS-1.1)	CVCL_C923 (BJ-RiPS-1.2)	CVCL_C8QN (BJ-TERT)
CVCL_C924 (BJ-RiPS-1.3)	CVCL_6573 (BJ1-hTERT)	CVCL_C921 (BJ1-iPS1)
CVCL_V235 (BJ1-iPS3)	CVCL_0G92 (BJC)	CVCL_0G93 (BJD)
CVCL_VU02 (BJFF.6)	CVCL_YR24 (CRTDi005-A)	CVCL_YR25 (CRTDi005-B)
CVCL_ZI69 (CTRL-iPSC)	CVCL_0G85 (eWT-BJ2)	CVCL_0G86 (eWT-BJ4)
CVCL_B3T9 (HHUUKDi009-A)	CVCL_QX15 (HNL1)	CVCL_RL51 (HNL2)
CVCL_RL52 (HNL4)	CVCL_RL53 (HNL5)	CVCL_RL54 (HNL6)
CVCL_RL55 (HNLp)	CVCL_C430 (IPS(Foreskin)-1)	CVCL_C431 (IPS(Foreskin)-2)
CVCL_C432 (IPS(Foreskin)-3)	CVCL_C433 (IPS(Foreskin)-4)	CVCL_YT30 (ISFi001-A)
CVCL_A8SS (ISFi002-A)	CVCL_ZZ36 (KKUi001-A)	CVCL_C1U7 (KULi003-A)
CVCL_A8MM (MILi001-A)	CVCL_B5IQ (MILi001-B)	CVCL_B5IR (MILi001-C)
CVCL_ZJ15 (MPIi005-A)	CVCL_YX99 (RCSIi001-A)	CVCL_VT49 (REGUi001-A)
CVCL_VT50 (REGUi002-A)	CVCL_RL56 (T10 [Human iPSC])	CVCL_RL57 (T11 [Human ESC])
CVCL_C1WY (UBi005-A)	CVCL_E5HD (UCH-HF-12)	CVCL_E5HE (UCH-HF-13)
CVCL_E5HF (UCH-HF-47)	CVCL_E5HG (UCH-HF-63)	CVCL_E5HH (UCH-HF-121)
CVCL_YR26 (UNIPDi004-A)	CVCL_YR27 (UNIPDi004-B)	CVCL_A5CE (UNIPDi007-A)
CVCL_D0PB (UTUi001-A)	CVCL_D0PC (UTUi001-B)	CVCL_D0PD (UTUi001-C)

Sex of cell

Male

Age at sampling

<1M

Category

Finite cell line

STR profile

Source(s): ATCC=CRL-2522; CLS=305222; PubMed=29444910

Markers:

Amelogenin	X,Y
CSF1PO	10,12
D2S1338	16,20
D3S1358	14,16
D5S818	12
D7S820	11,12
D8S1179	9,11
D13S317	8,9
D16S539	9,13
D18S51	17,19
D19S433	14
D21S11	29
FGA	22,23
Penta D	12,13
Penta E	7,17
TH01	7,8
TPOX	10,11
vWA	16,18

Web pages

Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/BJ_Stam_protocol.pdf
Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Info; StRAP; -; https://strap.nci.nih.gov/celline_detail.php?sample_id=67

Publications

PubMed=12554677; DOI=10.1093/hmg/ddg023
Andrei Tchirkov, Peter Michael Lansdorp;
Role of oxidative stress in telomere shortening in cultured fibroblasts from normal individuals and patients with ataxia-telangiectasia.
Hum. Mol. Genet. 12:227-232(2003)

PubMed=23325432; DOI=10.1101/gr.147942.112; PMCID=PMC3589544
Katherine E. Varley, Jason Gertz, Kevin M. Bowling, Stephanie L. Parker, Timothy E. Reddy, Florencia Pauli-Behn, Marie K. Cross, Brian A. Williams, John A. Stamatoyannopoulos, Gregory E. Crawford, Devin Michael Absher ...Show all 13 authors... , Barbara Jane Wold, Richard M. Myers; Show fewer authors
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=29444910; DOI=10.1530/ERC-17-0445; PMCID=PMC5827037
Tobias Hofving, Yvonne Arvidsson, Bilal Almobarak, Linda Inge, Roswitha Pfragner, Marta Persson, Goran Stenman, Erik Kristiansson, Viktor Johanson, Ola Nilsson;
The neuroendocrine phenotype, genomic profile and therapeutic sensitivity of GEPNET cell lines.
Endocr. Relat. Cancer 25:367-380(2018)

Cross-references

Cell line collections (Providers)

AddexBio; P0012005/4972
ATCC; CRL-2522
CLS; 305222
Ubigene; YC-C080

Cell line databases/resources

CLO; CLO_0001980
Cell_Model_Passport; SIDM01891
Lonza; 723
SKIP; SKIP001154
TOKU-E; 672

Anatomy/cell type resources

BTO; BTO_0003807

Biological sample resources

ENCODE; ENCBS070WLP
ENCODE; ENCBS074ENC
ENCODE; ENCBS137RRI
ENCODE; ENCBS170GUW
ENCODE; ENCBS198NMJ
ENCODE; ENCBS335XLL
ENCODE; ENCBS339MSO
ENCODE; ENCBS488ZLU
ENCODE; ENCBS610AAA
ENCODE; ENCBS611AAA
ENCODE; ENCBS612AAA
ENCODE; ENCBS613AAA
ENCODE; ENCBS614AAA
ENCODE; ENCBS615AAA
ENCODE; ENCBS616AAA
ENCODE; ENCBS628EGC
ENCODE; ENCBS683XYV
ENCODE; ENCBS693PNC
ENCODE; ENCBS753AAA
ENCODE; ENCBS754AAA
ENCODE; ENCBS772THG
ENCODE; ENCBS815JWZ
ENCODE; ENCBS911DVL
ENCODE; ENCBS935PZD

CRISP screens repositories

BioGRID_ORCS_Cell_line; 1125

Chemistry resources

ChEMBL-Cells; CHEMBL3307781
ChEMBL-Targets; CHEMBL614358
PubChem_Cell_line; CVCL_3653

Encyclopedic resources

Wikidata; Q54796903

Experimental variables resources

EFO; EFO_0002779

Gene expression databases

GEO; GSM248213
GEO; GSM248214
GEO; GSM472898
GEO; GSM472924
GEO; GSM472925
GEO; GSM579886
GEO; GSM579887
GEO; GSM736518
GEO; GSM736596
GEO; GSM749677
GEO; GSM749752
GEO; GSM749770
GEO; GSM923444
GEO; GSM945178
GEO; GSM945204
GEO; GSM945207
GEO; GSM945235
GEO; GSM2988896
GEO; GSM7701416
GEO; GSM7701417

Entry history

Entry creation

04-Apr-2012

Last entry update

10-Apr-2025

Version number