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Cellosaurus VM7Luc4E2 (CVCL_6571)

[Text version]
Cell line name VM7Luc4E2
Synonyms BG1Luc4E2; BG-1Luc4E2; BG1Luc4E(2); BG1-ER
Accession CVCL_6571
Resource Identification Initiative To cite this cell line use: VM7Luc4E2 (RRID:CVCL_6571)
Comments Problematic cell line: Contaminated. Shown to be a MCF-7 derivative (https://tsar.jrc.ec.europa.eu/test-method/tm2016-09). Originally thought to be a BG1 (Cellosaurus=CVCL_6570) derivative.
Population: Caucasian.
Biotechnology: Cell line developed for VM7Luc ER TA (VM7Luc Estrogen Receptor TransActivation) bioassay which was formerly known as BG1Luc ER TA (BG1Luc Estrogen Receptor TransActivation).
Biotechnology: Used in the Organisation for Economic Co-operation and Development (OECD) detection of estrogenic/antiestrogenic chemicals test method 457 (DOI=10.1787/9789264185395-en).
Characteristics: Emits measurable light in response to estrogen and estrogen-like substances. Used for identifying estrogen receptor agonists and antagonists.
Characteristics: Acts through the activation of a luciferase reporter gene under the control of estrogen response elements (EREs) upstream of the mouse mammary tumor viral (MMTV) promoter.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
CVCL_DD13 (BG1LucERbc9)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
Web pages http://www.dioxins.com/lumi2.html

Rogers J.M., Denison M.S.
Recombinant cell bioassays for endocrine disruptors: development of a stably transfected human ovarian cell line for the detection of estrogenic and anti-estrogenic chemicals.
In Vitro Mol. Toxicol. 13:67-82(2000)

Test No. 457: BG1Luc estrogen receptor transactivation test method for identifying estrogen receptor agonists and antagonists.
(In) OECD guidelines for the testing of chemicals, Section 4; pp.1-26; OECD Publishing; Paris (2012)

PubMed=26139245; DOI=10.1002/etc.3146
Brennan J.C., Bassal A., He G.-C., Denison M.S.
Development of a recombinant human ovarian (BG1) cell line containing estrogen receptor alpha and beta for improved detection of estrogenic/antiestrogenic chemicals.
Environ. Toxicol. Chem. 35:91-100(2016)

Encyclopedic resources Wikidata; Q54796232
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number22