Cellosaurus cell line VM7Luc4E2 (CVCL

Cellosaurus VM7Luc4E2 (CVCL_6571)

[Text][XML][JSON]

Cell line name

VM7Luc4E2

Synonyms

BG1Luc4E2; BG-1Luc4E2; BG1Luc4E(2); BG1-ER

Accession

CVCL_6571

Resource Identification Initiative

To cite this cell line use: VM7Luc4E2 (RRID:CVCL_6571)

Comments

Problematic cell line: Contaminated. Shown to be a MCF-7 derivative (EURL ECVAM-TSAR=TM2016-09). Originally thought to be a BG1 (Cellosaurus=CVCL_6570) derivative.
Population: Caucasian.
Biotechnology: Cell line developed for VM7Luc ER TA (VM7Luc Estrogen Receptor TransActivation) bioassay which was formerly known as BG1Luc ER TA (BG1Luc Estrogen Receptor TransActivation).
Biotechnology: Used in the Organisation for Economic Co-operation and Development (OECD) detection of estrogenic/antiestrogenic chemicals test method 457 (DOI=10.1787/9789264185395-en).
Characteristics: Emits measurable light in response to estrogen and estrogen-like substances. Used for identifying estrogen receptor agonists and antagonists.
Characteristics: Acts through the activation of a luciferase reporter gene under the control of estrogen response elements (EREs) upstream of the mouse mammary tumor viral (MMTV) promoter.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).

Disease

Invasive breast carcinoma of no special type (NCIt: C4194)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0031 (MCF-7)
Children:

CVCL_DD13 (BG1LucERbc9)

Sex of cell

Female

Age at sampling

69Y