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Cellosaurus D-54MG (CVCL_5735)

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Cell line name D-54MG
Synonyms D-54 MG; D54-MG; D54MG; D54Mg; D54; H54
Accession CVCL_5735
Secondary accession CVCL_7185
Resource Identification Initiative To cite this cell line use: D-54MG (RRID:CVCL_5735)
Comments Problematic cell line: Contaminated. Shown to be a A-172 derivative (ICLAC; personal communication of Bigner, Darell Doty). Originally thought to originate from a 39 year old female patient with a glioblastoma.
Part of: ENCODE project common cell types; tier 3.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00505.
Population: Caucasian.
Doubling time: 40.0 +- 3.6 hours (Note=In serum-supplemented media), 46.0 +- 0.7 hours (Note=In serum-free media) (PubMed=3178168).
Miscellaneous: STR profile from personal communication of Bigner, Darell Doty.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Gene deletion; HGNC; HGNC:9588; PTEN; Zygosity=Homozygous (PubMed=9090379).
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1539; CBFB; Name(s)=ABL1-CBFB (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0131 (A-172)
Children:
CVCL_E6ZL (D54MG fat-1)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO9,12
D2S133820,21
D3S135814,18
D5S81811,12
D7S82011
D8S117913,14
D13S31711
D16S53912
D18S5113
D19S43312,15.2
D21S1128,32.2
FGA20,22
TH016,9.3
TPOX8,11
vWA16,20

Run an STR similarity search on this cell line
Web pages Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/D54_Crawford_protocol.pdf
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Darell Doty Bigner, Sandra H. Bigner, Jan Ponten, Bengt Westermark, Moses Stephen Mahaley Jr., Erkki Ruoslahti, Harvey R. Herschman, Lawrence F. Eng, Carol J. Wikstrand;
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

PubMed=3675803
Sandra H. Bigner, Rolf Bjerkvig, Ole Didrik Laerum, Laurence H. Muhlbaier, Darell Doty Bigner;
DNA content and chromosomes in permanent cultured cell lines derived from malignant human gliomas.
Anal. Quant. Cytol. Histol. 9:435-444(1987)

PubMed=3178168
Lars Andreas Akslen, Knut-Jan Andersen, Rolf Bjerkvig;
Characteristics of human and rat glioma cells grown in a defined medium.
Anticancer Res. 8:797-803(1988)

DOI=10.1016/B978-0-12-333530-2.50005-8
Monica Nister, Bengt Westermark;
Human glioma cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.17-42; Academic Press; New York; USA (1994)

PubMed=9090379; DOI=10.1038/ng0497-356
Peter A. Steck, Mark A. Pershouse, Samar A. Jasser, Wai-Kwan Alfred Yung, Huai Lin, Azra Hadi Ligon, Lauren A. Langford, Michelle L. Baumgard, Thomas Hattier, Thaylon Davis, Cheryl Frye ...Show all 15 authors... , Rong Hu, Bradley Swedlund, David H.-F. Teng, Sean V. Tavtigian; Show fewer authors
Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.
Nat. Genet. 15:356-362(1997)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Taraneh R. Sharif, Wei Luo, Mohammed Sharif;
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

DOI=10.1007/0-306-46861-1_11
Francis Ali-Osman;
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=10402232; DOI=10.3892/ijo.15.2.237
Taraneh R. Sharif, Mohammed Sharif;
Overexpression of protein kinase C epsilon in astroglial brain tumor derived cell lines and primary tumor samples.
Int. J. Oncol. 15:237-243(1999)

Cross-references
Cell line databases/resources Lonza; 684
TOKU-E; 3722
Anatomy/cell type resources BTO; BTO_0005364
Biological sample resources BioSample; SAMN06481110
ENCODE; ENCBS173AAA
Chemistry resources ChEMBL-Cells; CHEMBL3308484
ChEMBL-Targets; CHEMBL612247
PubChem_Cell_line; CVCL_7185
Encyclopedic resources Wikidata; Q54817140
Experimental variables resources EFO; EFO_0002782
Gene expression databases GEO; GSM816668
GEO; GSM881397
GEO; GSM881398
Polymorphism and mutation databases Cosmic; 970092
Cosmic; 1672410
IARC_TP53; 23935
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number31