Cellosaurus logo
expasy logo

Cellosaurus D-54MG (CVCL_5735)

[Text version]
Cell line name D-54MG
Synonyms D-54 MG; D54-MG; D54MG; D54Mg; D54; H54
Accession CVCL_5735
Secondary accession CVCL_7185
Resource Identification Initiative To cite this cell line use: D-54MG (RRID:CVCL_5735)
Comments Problematic cell line: Contaminated. Shown to be a A-172 derivative (ICLAC; personal communication of Bigner D.D.). Originally thought to originate from a 39 year old female patient with a glioblastoma.
Part of: ENCODE project common cell types; tier 3.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00505.
Population: Caucasian.
Doubling time: 40.0 +- 3.6 hours (Note=In serum-supplemented media), 46.0 +- 0.7 hours (Note=In serum-free media) (PubMed=3178168).
Miscellaneous: STR profile from personal communication of Bigner D.D.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (PubMed=9090379).
  • Gene fusion; HGNC; 76; ABL1 + HGNC; 1539; CBFB; Name(s)=ABL1-CBFB (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0131 (A-172)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): Direct_author_submission


Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Bigner D.D., Bigner S.H., Ponten J., Westermark B., Mahaley M.S. Jr., Ruoslahti E., Herschman H., Eng L.F., Wikstrand C.J.
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

Bigner S.H., Bjerkvig R., Laerum O.D., Muhlbaier L.H., Bigner D.D.
DNA content and chromosomes in permanent cultured cell lines derived from malignant human gliomas.
Anal. Quant. Cytol. Histol. 9:435-444(1987)

Akslen L.A., Andersen K.-J., Bjerkvig R.
Characteristics of human and rat glioma cells grown in a defined medium.
Anticancer Res. 8:797-803(1988)

Nister M., Westermark B.
Human glioma cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York (1994)

PubMed=9090379; DOI=10.1038/ng0497-356
Steck P.A., Pershouse M.A., Jasser S.A., Yung W.-K.A., Lin H., Ligon A.H., Langford L.A., Baumgard M.L., Hattier T., Davis T., Frye C., Hu R., Swedlund B., Teng D.H.-F., Tavtigian S.V.
Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.
Nat. Genet. 15:356-362(1997)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Sharif T.R., Luo W., Sharif M.
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

Ali-Osman F.
Brain tumors.
(In) Human cell culture. Vol. 2. Cancer Cell Lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York (1999)

PubMed=10402232; DOI=10.3892/ijo.15.2.237
Sharif T.R., Sharif M.
Overexpression of protein kinase C epsilon in astroglial brain tumor derived cell lines and primary tumor samples.
Int. J. Oncol. 15:237-243(1999)

Cell line databases/resources Lonza; 684
TOKU-E; 3722
Anatomy/cell type resources BTO; BTO:0005364
Biological sample resources BioSample; SAMN06481110
Chemistry resources ChEMBL-Cells; CHEMBL3308484
ChEMBL-Targets; CHEMBL612247
PubChem_Cell_line; CVCL_7185
Encyclopedic resources Wikidata; Q54817140
Experimental variables resources EFO; EFO_0002782
Gene expression databases GEO; GSM816668
GEO; GSM881397
GEO; GSM881398
Polymorphism and mutation databases Cosmic; 970092
Cosmic; 1672410
IARC_TP53; 23935
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number27