ID   D-54MG
AC   CVCL_5735
AS   CVCL_7185
SY   D-54 MG; D54-MG; D54MG; D54Mg; D54; H54
DR   BTO; BTO_0005364
DR   EFO; EFO_0002782
DR   BioSample; SAMN06481110
DR   ChEMBL-Cells; CHEMBL3308484
DR   ChEMBL-Targets; CHEMBL612247
DR   Cosmic; 970092
DR   Cosmic; 1672410
DR   ENCODE; ENCBS173AAA
DR   GEO; GSM816668
DR   GEO; GSM881397
DR   GEO; GSM881398
DR   IARC_TP53; 23935
DR   Lonza; 684
DR   PubChem_Cell_line; CVCL_7185
DR   TOKU-E; 3722
DR   Wikidata; Q54817140
RX   DOI=10.1007/0-306-46861-1_11;
RX   DOI=10.1016/B978-0-12-333530-2.50005-8;
RX   PubMed=3178168;
RX   PubMed=3675803;
RX   PubMed=6260907;
RX   PubMed=9090379;
RX   PubMed=9220028;
RX   PubMed=10402232;
WW   Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/D54_Crawford_protocol.pdf
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a A-172 derivative (ICLAC; personal communication of Bigner, Darell Doty). Originally thought to originate from a 39 year old female patient with a glioblastoma.
CC   Part of: ENCODE project common cell types; tier 3.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00505.
CC   Population: Caucasian.
CC   Doubling time: 40.0 +- 3.6 hours (Note=In serum-supplemented media), 46.0 +- 0.7 hours (Note=In serum-free media) (PubMed=3178168).
CC   Sequence variation: Gene deletion; HGNC; HGNC:9588; PTEN; Zygosity=Homozygous (PubMed=9090379).
CC   Sequence variation: Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1539; CBFB; Name(s)=ABL1-CBFB (from parent cell line).
CC   Miscellaneous: STR profile from personal communication of Bigner, Darell Doty.
CC   Derived from site: In situ; Brain; UBERON=UBERON_0000955.
ST   Source(s): Direct_author_submission
ST   Amelogenin: X,Y
ST   CSF1PO: 9,12
ST   D13S317: 11
ST   D16S539: 12
ST   D18S51: 13
ST   D19S433: 12,15.2
ST   D21S11: 28,32.2
ST   D2S1338: 20,21
ST   D3S1358: 14,18
ST   D5S818: 11,12
ST   D7S820: 11
ST   D8S1179: 13,14
ST   FGA: 20,22
ST   TH01: 6,9.3
ST   TPOX: 8,11
ST   vWA: 16,20
DI   NCIt; C3058; Glioblastoma
DI   ORDO; Orphanet_360; Glioblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0131 ! A-172
SX   Male
AG   53Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 31
CH   CVCL_E6ZL ! D54MG fat-1
//
RX   DOI=10.1007/0-306-46861-1_11;
RA   Ali-Osman, Francis
RT   "Brain tumors.";
RL   (In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999).
//
RX   PubMed=9090379; DOI=10.1038/ng0497-356;
RA   Steck, Peter A.
RA   Pershouse, Mark A.
RA   Jasser, Samar A.
RA   Yung, Wai-Kwan Alfred
RA   Lin, Huai
RA   Ligon, Azra Hadi
RA   Langford, Lauren A.
RA   Baumgard, Michelle L.
RA   Hattier, Thomas
RA   Davis, Thaylon
RA   Frye, Cheryl
RA   Hu, Rong
RA   Swedlund, Bradley
RA   Teng, David H.-F.
RA   Tavtigian, Sean V.
RT   "Identification of a candidate tumour suppressor gene, MMAC1, at
RT   chromosome 10q23.3 that is mutated in multiple advanced cancers.";
RL   Nat. Genet. 15:356-362(1997).
//
RX   PubMed=6260907; DOI=10.1097/00005072-198105000-00001;
RA   Bigner, Darell Doty
RA   Bigner, Sandra H.
RA   Ponten, Jan
RA   Westermark, Bengt
RA   Mahaley, Moses Stephen Jr.
RA   Ruoslahti, Erkki
RA   Herschman, Harvey R.
RA   Eng, Lawrence F.
RA   Wikstrand, Carol J.
RT   "Heterogeneity of genotypic and phenotypic characteristics of fifteen
RT   permanent cell lines derived from human gliomas.";
RL   J. Neuropathol. Exp. Neurol. 40:201-229(1981).
//
RX   DOI=10.1016/B978-0-12-333530-2.50005-8;
RA   Nister, Monica
RA   Westermark, Bengt
RT   "Human glioma cell lines.";
RL   (In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.17-42; Academic Press; New York; USA (1994).
//
RX   PubMed=10402232; DOI=10.3892/ijo.15.2.237;
RA   Sharif, Taraneh R.
RA   Sharif, Mohammed
RT   "Overexpression of protein kinase C epsilon in astroglial brain tumor
RT   derived cell lines and primary tumor samples.";
RL   Int. J. Oncol. 15:237-243(1999).
//
RX   PubMed=3675803;
RA   Bigner, Sandra H.
RA   Bjerkvig, Rolf
RA   Laerum, Ole Didrik
RA   Muhlbaier, Laurence H.
RA   Bigner, Darell Doty
RT   "DNA content and chromosomes in permanent cultured cell lines derived
RT   from malignant human gliomas.";
RL   Anal. Quant. Cytol. Histol. 9:435-444(1987).
//
RX   PubMed=3178168;
RA   Akslen, Lars Andreas
RA   Andersen, Knut-Jan
RA   Bjerkvig, Rolf
RT   "Characteristics of human and rat glioma cells grown in a defined
RT   medium.";
RL   Anticancer Res. 8:797-803(1988).
//
RX   PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4;
RA   Sharif, Taraneh R.
RA   Luo, Wei
RA   Sharif, Mohammed
RT   "Functional expression of bombesin receptor in most adult and
RT   pediatric human glioblastoma cell lines; role in mitogenesis and in
RT   stimulating the mitogen-activated protein kinase pathway.";
RL   Mol. Cell. Endocrinol. 130:119-130(1997).
//