ID   D-54MG
AC   CVCL_5735
AS   CVCL_7185
SY   D-54 MG; D54-MG; D54MG; D54Mg; D54; H54
DR   BTO; BTO:0005364
DR   EFO; EFO_0002782
DR   BioSample; SAMN06481110
DR   ChEMBL-Cells; CHEMBL3308484
DR   ChEMBL-Targets; CHEMBL612247
DR   Cosmic; 970092
DR   Cosmic; 1672410
DR   GEO; GSM816668
DR   GEO; GSM881397
DR   GEO; GSM881398
DR   IARC_TP53; 23935
DR   Lonza; 684
DR   PubChem_Cell_line; CVCL_7185
DR   TOKU-E; 3722
DR   Wikidata; Q54817140
RX   DOI=10.1007/0-306-46861-1_11;
RX   DOI=10.1016/B978-0-12-333530-2.50005-8;
RX   PubMed=3178168;
RX   PubMed=3675803;
RX   PubMed=6260907;
RX   PubMed=9090379;
RX   PubMed=9220028;
RX   PubMed=10402232;
CC   Problematic cell line: Contaminated. Shown to be a A-172 derivative (ICLAC; personal communication of Bigner D.D.). Originally thought to originate from a 39 year old female patient with a glioblastoma.
CC   Part of: ENCODE project common cell types; tier 3.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00505.
CC   Population: Caucasian.
CC   Doubling time: 40.0 +- 3.6 hours (Note=In serum-supplemented media), 46.0 +- 0.7 hours (Note=In serum-free media) (PubMed=3178168).
CC   Sequence variation: Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (PubMed=9090379).
CC   Sequence variation: Gene fusion; HGNC; 76; ABL1 + HGNC; 1539; CBFB; Name(s)=ABL1-CBFB (from parent cell line).
CC   Miscellaneous: STR profile from personal communication of Bigner D.D.
CC   Derived from site: In situ; Brain; UBERON=UBERON_0000955.
ST   Source(s): Direct_author_submission
ST   Amelogenin: X,Y
ST   CSF1PO: 9,12
ST   D13S317: 11
ST   D16S539: 12
ST   D18S51: 13
ST   D19S433: 12,15.2
ST   D21S11: 28,32.2
ST   D2S1338: 20,21
ST   D3S1358: 14,18
ST   D5S818: 11,12
ST   D7S820: 11
ST   D8S1179: 13,14
ST   FGA: 20,22
ST   TH01: 6,9.3
ST   TPOX: 8,11
ST   vWA: 16,20
DI   NCIt; C3058; Glioblastoma
DI   ORDO; Orphanet_360; Glioblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0131 ! A-172
SX   Male
AG   53Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 28
RX   DOI=10.1007/0-306-46861-1_11;
RA   Ali-Osman F.;
RT   "Brain tumors.";
RL   (In) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York (1999).
RX   DOI=10.1016/B978-0-12-333530-2.50005-8;
RA   Nister M., Westermark B.;
RT   "Human glioma cell lines.";
RL   (In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York (1994).
RX   PubMed=3178168;
RA   Akslen L.A., Andersen K.-J., Bjerkvig R.;
RT   "Characteristics of human and rat glioma cells grown in a defined
RT   medium.";
RL   Anticancer Res. 8:797-803(1988).
RX   PubMed=3675803;
RA   Bigner S.H., Bjerkvig R., Laerum O.D., Muhlbaier L.H., Bigner D.D.;
RT   "DNA content and chromosomes in permanent cultured cell lines derived
RT   from malignant human gliomas.";
RL   Anal. Quant. Cytol. Histol. 9:435-444(1987).
RX   PubMed=6260907; DOI=10.1097/00005072-198105000-00001;
RA   Bigner D.D., Bigner S.H., Ponten J., Westermark B., Mahaley M.S. Jr.,
RA   Ruoslahti E., Herschman H., Eng L.F., Wikstrand C.J.;
RT   "Heterogeneity of genotypic and phenotypic characteristics of fifteen
RT   permanent cell lines derived from human gliomas.";
RL   J. Neuropathol. Exp. Neurol. 40:201-229(1981).
RX   PubMed=9090379; DOI=10.1038/ng0497-356;
RA   Steck P.A., Pershouse M.A., Jasser S.A., Yung W.-K.A., Lin H.,
RA   Ligon A.H., Langford L.A., Baumgard M.L., Hattier T., Davis T.,
RA   Frye C., Hu R., Swedlund B., Teng D.H.-F., Tavtigian S.V.;
RT   "Identification of a candidate tumour suppressor gene, MMAC1, at
RT   chromosome 10q23.3 that is mutated in multiple advanced cancers.";
RL   Nat. Genet. 15:356-362(1997).
RX   PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4;
RA   Sharif T.R., Luo W., Sharif M.;
RT   "Functional expression of bombesin receptor in most adult and
RT   pediatric human glioblastoma cell lines; role in mitogenesis and in
RT   stimulating the mitogen-activated protein kinase pathway.";
RL   Mol. Cell. Endocrinol. 130:119-130(1997).
RX   PubMed=10402232; DOI=10.3892/ijo.15.2.237;
RA   Sharif T.R., Sharif M.;
RT   "Overexpression of protein kinase C epsilon in astroglial brain tumor
RT   derived cell lines and primary tumor samples.";
RL   Int. J. Oncol. 15:237-243(1999).