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Cellosaurus OP9 (CVCL_4398)

[Text version]

Cell line name OP9
Synonyms OP-9
Accession CVCL_4398
Resource Identification Initiative To cite this cell line use: OP9 (RRID:CVCL_4398)
Comments Characteristics: Does not produce Csf1 (M-CSF) due to a mutation in the gene.
Characteristics: Used as feeder cells for the differentiation of ES cells and iPS cells into hematopoietic cells.
Derived from sampling site: Bone marrow. Cell type=Mesenchymal stem cell.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6 x C3H) F2-op/op.
Hierarchy Children:
CVCL_B218 (OP9/G-DLL1)CVCL_B219 (OP9/N)CVCL_B220 (OP9/N-DLL1)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Stromal cell line
Web pages https://en.wikipedia.org/wiki/OP9_cell

PubMed=8066449; DOI=10.1126/science.8066449
Nakano T., Kodama H., Honjo T.
Generation of lymphohematopoietic cells from embryonic stem cells in culture.
Science 265:1098-1101(1994)

Nakano T.
In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis.
Int. J. Hematol. 65:1-8(1996)

PubMed=20659712; DOI=10.1016/S1673-8527(09)60067-9
Gao J., Yan X.-L., Li R., Liu Y., He W.-Y., Sun S.-K., Zhang Y., Liu B., Xiong J.-X., Mao N.
Characterization of OP9 as authentic mesenchymal stem cell line.
J. Genet. Genomics 37:475-482(2010)

PubMed=20810995; DOI=10.4049/jimmunol.1000195
Murata A., Okuyama K., Sakano S., Kajiki M., Hirata T., Yagita H., Zuniga-Pflucker J.C., Miyake K., Akashi-Takamura S., Moriwaki S., Niida S., Yoshino M., Hayashi S.
A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells.
J. Immunol. 185:3905-3912(2010)

PubMed=23469264; DOI=10.1371/journal.pone.0058123
Matsubara Y., Ono Y., Suzuki H., Arai F., Suda T., Murata M., Ikeda Y.
OP9 bone marrow stroma cells differentiate into megakaryocytes and platelets.
PLoS ONE 8:E58123-E58123(2013)

PubMed=28176856; DOI=10.1038/srep42104
Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.
Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.
Sci. Rep. 7:42104-42104(2017)

Cell line collections ATCC; CRL-2749
BCRC; 60566
BCRJ; 0309
CLS; 305174
RCB; RCB1124
Cell line databases/resources CLO; CLO_0008268
CCRID; 1101MOU-PUMC000479
Anatomy/cell type resources BTO; BTO:0003295
Encyclopedic resources Wikidata; Q50871278
Gene expression databases GEO; GSM562062
Entry history
Entry creation04-Apr-2012
Last entry update15-Dec-2022
Version number18