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Cellosaurus OP9 (CVCL_4398)

[Text version]
Cell line name OP9
Synonyms OP-9
Accession CVCL_4398
Resource Identification Initiative To cite this cell line use: OP9 (RRID:CVCL_4398)
Comments Characteristics: Does not produce Csf1 (M-CSF) due to a mutation in the gene.
Characteristics: Used as feeder cells for the differentiation of ES cells and iPS cells into hematopoietic cells.
Doubling time: ~26 hours (ATCC=CRL-2749).
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6 x C3H) F2-op/op.
Hierarchy Children:
CVCL_HC57 (OP-9S)CVCL_KB57 (OP9-K)CVCL_B217 (OP9/G)
CVCL_B218 (OP9/G-DLL1)CVCL_B219 (OP9/N)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Stromal cell line
Web pages Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=op9
Info; Wikipedia; -; https://en.wikipedia.org/wiki/OP9_cell
Publications

PubMed=8066449; DOI=10.1126/science.8066449
Toru Nakano, Hiroaki Kodama, Tasuku Honjo;
Generation of lymphohematopoietic cells from embryonic stem cells in culture.
Science 265:1098-1101(1994)

PubMed=8990620; DOI=10.1016/s0925-5710(96)00531-2
Toru Nakano;
In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis.
Int. J. Hematol. 65:1-8(1996)

PubMed=20659712; DOI=10.1016/S1673-8527(09)60067-9
Jiao Gao, Xin-Long Yan, Ren Li, Yi Liu, Wen-Yan He, Sheng-Kun Sun, Yu Zhang, Bing Liu, Jia-Xiang Xiong, Ning Mao;
Characterization of OP9 as authentic mesenchymal stem cell line.
J. Genet. Genomics 37:475-482(2010)

PubMed=20810995; DOI=10.4049/jimmunol.1000195
Akihiko Murata, Kazuki Okuyama, Seiji Sakano, Masahiro Kajiki, Tomohisa Hirata, Hideo Yagita, Juan Carlos Zuniga-Pflucker, Kensuke Miyake, Sachiko Akashi-Takamura, Sawako Moriwaki, Shumpei Niida ...Show all 13 authors... , Miya Yoshino, Shin-ichi Hayashi; Show fewer authors
A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells.
J. Immunol. 185:3905-3912(2010)

PubMed=23469264; DOI=10.1371/journal.pone.0058123; PMCID=PMC3585802
Yumiko Matsubara, Yukako Ono, Hidenori Suzuki, Fumio Arai, Toshio Suda, Mitsuru Murata, Yasuo Ikeda;
OP9 bone marrow stroma cells differentiate into megakaryocytes and platelets.
PLoS ONE 8:e58123.1-e58123.9(2013)

PubMed=28176856; DOI=10.1038/srep42104; PMCID=PMC5296734
Christopher D. Kassotis, Lauren Masse, Stephanie Kim, Jennifer Joy Schlezinger, Thomas F. Webster, Heather M. Stapleton;
Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.
Sci. Rep. 7:42104-42104(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-2749
BCRC; 60566
BCRJ; 0309
CLS; 305174
RCB; RCB1124
Cell line databases/resources CLO; CLO_0008268
CCRID; 1101MOU-PUMC000479
CCRID; 3101MOUGNM17
CCRID; 3101MOUSCSP227
Anatomy/cell type resources BTO; BTO_0003295
Encyclopedic resources Wikidata; Q50871278
Gene expression databases GEO; GSM562062
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number23