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Cellosaurus U87/DK (CVCL_3428)

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Cell line name U87/DK
Synonyms U87-DK; U87DK; U87 dead kinase; U87MG.deltaEGFR DK; U87MG.DK
Accession CVCL_3428
Resource Identification Initiative To cite this cell line use: U87/DK (RRID:CVCL_3428)
Comments Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
Population: Caucasian.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3236; EGFR (Note=With p.Val30_Arg297del and p.Lys745Met = EGFRvIII and dead kinase).
Sequence variations
  • Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0022 (U-87MG ATCC)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): ICLC=HTL08003

Markers:
AmelogeninX
CSF1PO10,11
D5S81811,12
D7S8208,9
D13S31711
D16S53912
D21S1128.2,32.2
TH019.3
TPOX8
vWA15,17

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Publications

PubMed=8810340; DOI=10.1074/jbc.271.41.25639
Sally A. Prigent, Motoo Nagane, Hong Lin, Ivana Huvar, Gerry R. Boss, James Robert Feramisco, Webster K. Cavenee, H.-J. Su Huang;
Enhanced tumorigenic behavior of glioblastoma cells expressing a truncated epidermal growth factor receptor is mediated through the Ras-Shc-Grb2 pathway.
J. Biol. Chem. 271:25639-25645(1996)

PubMed=9006938; DOI=10.1074/jbc.272.5.2927
H.-J. Su Huang, Motoo Nagane, Candice K. Klingbeil, Hong Lin, Ryo Nishikawa, Xiang-Dong Ji, Chun-Ming Huang, Gordon Nelson Gill, H. Steven Wiley, Webster K. Cavenee;
The enhanced tumorigenic activity of a mutant epidermal growth factor receptor common in human cancers is mediated by threshold levels of constitutive tyrosine phosphorylation and unattenuated signaling.
J. Biol. Chem. 272:2927-2935(1997)

Cross-references
Cell line collections (Providers) ICLC; HTL08003
Cell line databases/resources CLDB; cl7208
cancercelllines; CVCL_3428
Encyclopedic resources Wikidata; Q54973750
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number22