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Cellosaurus WM266-4 (CVCL_2765)

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Cell line name WM266-4
Synonyms WM-266-4; WM 266-4; WM-2664; WM2664; WM 2664; WM266; WM-266-mel; WM266mel; WC00097
Accession CVCL_2765
Resource Identification Initiative To cite this cell line use: WM266-4 (RRID:CVCL_2765)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: MD Anderson Cell Lines Project.
Part of: Wistar Institute melanoma cell line collection.
Population: Caucasian.
Doubling time: ~30 hours (PBCF).
Karyotypic information: Hypertriploid karyotype.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Right thigh, skin; UBERON=UBERON_0004262.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (Wistar).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (Wistar).
  • Gene deletion; HGNC; HGNC:9588; PTEN; Zygosity=Hemizygous (Wistar).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Asp (c.1799_1800delTGinsAT); ClinVar=VCV000375939; Zygosity=Heterozygous (PubMed=12068308; PubMed=19799798; PubMed=23851445; PubMed=29492214; ATCC=CRL-1676; DepMap=ACH-001239; Wistar).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
HLA typing Source: PubMed=15592718
Class I
HLA-AA*02:01,29:02
HLA-BB*13:02,44:03:01
HLA-CC*06:02,16:01
Class II
HLA-DPDPB1*02:01:02,02:01:02
HLA-DQDQB1*02:01,02:01
HLA-DRDRB1*07:01,07:01

Source: PubMed=25960936
Class I
HLA-AA*02:01,29:01
HLA-BB*13:02,44:02
HLA-CC*06:02,06:02
Class II
HLA-DQDQB1*06:04,06:04
HLA-DRDRB1*07:01,14:103

Source: PubMed=26589293
Class I
HLA-AA*02:01,29:02
HLA-BB*13:02,44:03
HLA-CC*06:02,16:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.04
Native American0
East Asian, North0
East Asian, South0.62
South Asian0.32
European, North66.56
European, South32.46
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_0040 ! WM115
CVCL_L033 ! WM165-1
CVCL_RN55 ! WM165-2
CVCL_6795 ! WM239A
CVCL_RN56 ! WM239B
CVCL_RN57 ! WM266-1
CVCL_RN58 ! WM266-2
CVCL_RN59 ! WM266-3
Sex of cell Female
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1676; ESTDAB=ESTDAB-076; KCLB=21676; Wistar

Markers:
AmelogeninX
CSF1PO12
D2S133819
D3S135815,18
D5S81813
D7S8208
D8S117913,15
D13S31712,13
D16S53911,12
D18S5121
D19S43313
D21S1129
FGA21,23
TH017,9
TPOX8,11
vWA15,17

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Info; Synapse; PS-ON; https://www.synapse.org/#!Synapse:syn31544557
Info; Wistar; -; https://www.wistar.org/our-scientists/meenhard-herlyn
Publications

PubMed=3856042; DOI=10.1093/jnci/74.2.283
Meenhard Herlyn, Gloria Brady Balaban-Malenbaum, Jeannette L. Bennicelli, DuPont Guerry 4th, Ruth Halaban, Dorothee Herlyn, David E. Elder, Gerd G. Maul, Zenon Steplewski, Peter Carey Nowell, Wallace H. Clark Jr., Hilary Koprowski;
Primary melanoma cells of the vertical growth phase: similarities to metastatic cells.
J. Natl. Cancer Inst. 74:283-289(1985)

PubMed=4053039
Meenhard Herlyn, Jan Thurin, Gloria Brady Balaban-Malenbaum, Jeannette L. Bennicelli, Dorothee Herlyn, David E. Elder, Edward Bondi, DuPont Guerry 4th, Peter Carey Nowell, Wallace H. Clark Jr., Hilary Koprowski;
Characteristics of cultured human melanocytes isolated from different stages of tumor progression.
Cancer Res. 45:5670-5676(1985)

PubMed=3020539; DOI=10.1073/pnas.83.19.7197; PMCID=PMC386682
Bengt Westermark, Anders Johnsson, Ylva Paulsson, Christer Betsholtz, Carl-Henrik Heldin, Meenhard Herlyn, Ulrich Rodeck, Hilary Koprowski;
Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor (PDGF) and produce a PDGF-like growth factor.
Proc. Natl. Acad. Sci. U.S.A. 83:7197-7200(1986)

PubMed=2253310; DOI=10.1007/BF00046337
Meenhard Herlyn;
Human melanoma: development and progression.
Cancer Metastasis Rev. 9:101-112(1990)

PubMed=11121133; DOI=10.1046/j.1523-1747.2000.00199.x
Pedro M. Lacal, Cristina M. Failla, Elena Pagani, Teresa Odorisio, Cataldo Schietroma, Sabrina Falcinelli, Giovanna Zambruno, Stefania D'Atri;
Human melanoma cells secrete and respond to placenta growth factor and vascular endothelial growth factor.
J. Invest. Dermatol. 115:1000-1007(2000)

PubMed=12068308; DOI=10.1038/nature00766
Helen R. Davies, Graham Robert Bignell, Charles Cox, Philip J. Stephens, Sarah Edkins, Sheila Clegg, Jon W. Teague, Hayley Woffendin, Mathew J. Garnett, William Bottomley, Neil Davis ...Show all 52 authors... , Ed Dicks, Rebecca Ewing, Yvonne Floyd, Kristian Gray, Sarah Hall, Rachel Hawes, Jaime Hughes, Vivian Kosmidou, Andrew Menzies, Catherine Mould, Adrian Parker, Claire Stevens, Stephen Watt, Steven Hooper, Rebecca Wilson, Hiran Jayatilake, Barry Austin Gusterson, Colin S. Cooper, Janet M. Shipley, Darren Hargrave, Kathy Pritchard-Jones, Norman J. Maitland, Georgia Chenevix-Trench, Gregory J. Riggins, Darell Doty Bigner, Giuseppe Palmieri, Antonio Cossu, Adrienne M. Flanagan, Andrew Nicholson, Judy W.C. Ho, Suet Yi Leung, Siu Tsan Yuen, Barbara L. Weber, Hilliard Foster Seigler, Timothy L. Darrow, Hugh F. Paterson, Richard Marais, Christopher John Marshall, Richard Wooster, Michael Rudolf Stratton, P. Andrew Futreal; Show fewer authors
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=15467732; DOI=10.1038/sj.onc.1208152
Hideaki Tanami, Issei Imoto, Akira Hirasawa, Yasuhiro Yuki, Itaru Sonoda, Jun Inoue, Kohichiroh Yasui, Akiko Misawa-Furihata, Yutaka Kawakami, Johji Inazawa;
Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.
Oncogene 23:8796-8804(2004)

PubMed=15592718; DOI=10.1007/s00262-004-0561-5; PMCID=PMC11032966
Teresa Rodriguez, Rosa Mendez, Chrissy H. Roberts, Francisco Ruiz-Cabello Osuna, I. Anthony Dodi, Miguel Angel Lopez-Nevot, Laura Paco, Isabel Maleno, Steven G.E. Marsh, Graham Pawelec, Federico Garrido;
High frequency of homozygosity of the HLA region in melanoma cell lines reveals a pattern compatible with extensive loss of heterozygosity.
Cancer Immunol. Immunother. 54:141-148(2005)

PubMed=17308088; DOI=10.1158/0008-5472.CAN-06-3311
Janiel M. Shields, Nancy E. Thomas, Melissa Cregger, Aaron J. Berger, Michael Leslie, Chad Torrice, Hong-Lin Hao, Shannon Penland, Jack Leonard Arbiser, Glynis A. Scott, Tong Zhou ...Show all 17 authors... , Menashe Bar-Eli, James E. Bear, Channing Joseph Der, William K. Kaufmann, David L. Rimm, Norman Edward Sharpless; Show fewer authors
Lack of extracellular signal-regulated kinase mitogen-activated protein kinase signaling shows a new type of melanoma.
Cancer Res. 67:1502-1512(2007)

PubMed=19799798; DOI=10.1186/1471-2407-9-352; PMCID=PMC2763007
Milena Casula, Antonio Muggiano, Antonio Cossu, Mario Budroni, Corrado Caraco, Paolo Antonio Ascierto, Elena Pagani, Ignazio Stanganelli, Sergio Canzanella, Maria Cristina Sini, Grazia Palomba, Giuseppe Palmieri;
Italian Melanoma Intergroup (IMI)
Role of key-regulator genes in melanoma susceptibility and pathogenesis among patients from South Italy.
BMC Cancer 9:352.1-352.11(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21424129; DOI=10.3892/or.2011.1220
Antonella Manca, Maria Cristina Sini, Francesco Izzo, Paolo Antonio Ascierto, Fabiana Tatangelo, Gerardo Botti, Giusy Gentilcore, Marilena Capone, Nicola Mozzillo, Carla Rozzo, Antonio Cossu ...Show all 13 authors... , Francesco Tanda, Giuseppe Palmieri; Show fewer authors
Induction of arginosuccinate synthetase (ASS) expression affects the antiproliferative activity of arginine deiminase (ADI) in melanoma cells.
Oncol. Rep. 25:1495-1502(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23851445; DOI=10.1158/1541-7786.MCR-13-0006
Christina Dahl, Claus Christensen, Goran Jonsson, Anders Lorentzen, Mette Louise Skjodt, Ake Borg, Graham Pawelec, Per Guldberg;
Mutual exclusivity analysis of genetic and epigenetic drivers in melanoma identifies a link between p14 ARF and RARbeta signaling.
Mol. Cancer Res. 11:1166-1178(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893; PMCID=PMC4355981
Sebastian Boegel, Martin Lower, Thomas Bukur, Ugur Sahin, John C. Castle;
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=28158294; DOI=10.1371/journal.pone.0171512; PMCID=PMC5291375
Eumorphia G. Konstantakou, Athanassios D. Velentzas, Athanasios K. Anagnostopoulos, Zoi I. Litou, Ourania A. Konstandi, Aikaterini F. Giannopoulou, Ema Anastasiadou, Gerassimos E. Voutsinas, George T. Tsangaris, Dimitrios J. Stravopodis;
Deep-proteome mapping of WM-266-4 human metastatic melanoma cells: from oncogenic addiction to druggable targets.
PLoS ONE 12:e0171512.1-e0171512.32(2017)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29492214; DOI=10.18632/oncotarget.23989; PMCID=PMC5823576
Maria Cristina Sini, Valentina Doneddu, Panagiotis Paliogiannis, Milena Casula, Maria Colombino, Antonella Manca, Gerardo Botti, Paolo Antonio Ascierto, Amelia Lissia, Antonio Cossu, Giuseppe Palmieri;
Genetic alterations in main candidate genes during melanoma progression.
Oncotarget 9:8531-8541(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections (Providers) ATCC; CRL-1676 - Discontinued
Coriell; WC00097 - Discontinued
ECACC; 91061233 - Discontinued
KCLB; 21676
Rockland; WM266-4-01-0001
Cell line databases/resources CLO; CLO_0009612
CLO; CLO_0009615
CLDB; cl4726
cancercelllines; CVCL_2765
Cell_Model_Passport; SIDM00979
CGH-DB; 9312-4
DepMap; ACH-001239
ESTDAB; ESTDAB-076
LINCS_LDP; LCL-1259
Lonza; 1375
Anatomy/cell type resources BTO; BTO_0001595
Biological sample resources BioSample; SAMN05292442
BioSample; SAMN10988367
CRISP screens repositories BioGRID_ORCS_Cell_line; 344
Chemistry resources ChEMBL-Cells; CHEMBL4295431
ChEMBL-Targets; CHEMBL4296510
PharmacoDB; WM2664_1663_2019
PubChem_Cell_line; CVCL_2765
Encyclopedic resources Wikidata; Q54994222
Experimental variables resources EFO; EFO_0006774
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSE17551
GEO; GSM827418
GEO; GSM887737
GEO; GSM888832
GEO; GSM1138792
GEO; GSM1670578
Polymorphism and mutation databases Cosmic; 687444
Cosmic; 905225
Cosmic; 932718
Cosmic; 972276
Cosmic; 1022277
Cosmic; 1054856
Cosmic; 1155273
Cosmic; 1238103
Cosmic; 1295343
Cosmic; 1303070
Cosmic; 1458970
Cosmic; 1459638
Cosmic; 1477409
Cosmic; 1507624
Cosmic; 1949557
Cosmic; 2163804
Cosmic; 2233687
Cosmic; 2651885
IARC_TP53; 30188
LiGeA; CCLE_891
Progenetix; CVCL_2765
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
EGA; EGAS00001002554
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number48