Cellosaurus logo
expasy logo

Cellosaurus WiDr (CVCL_2760)

[Text version]
Cell line name WiDr
Synonyms WiDR; WIDR; WiDr/S; WiDr-TC; WiDrTC; LED-WiDr; Led-WiDr
Accession CVCL_2760
Resource Identification Initiative To cite this cell line use: WiDr (RRID:CVCL_2760)
Comments Problematic cell line: Contaminated. Shown to be a HT-29 derivative (PubMed=3472642; PubMed=20143388). Originally thought to originate from a 78 year old female patient with a primary adenocarcinoma of the rectosigmoid colon.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00103.
Population: Caucasian.
Doubling time: 15 hours (PubMed=90012); 18 hours (PubMed=9488600); 45.68 hours (PubMed=25944804).
Microsatellite instability: Stable (MSS) (PubMed=10674020; PubMed=24042735; PubMed=25926053; PubMed=28683746).
Omics: Genomics; Whole exome sequencing.
Omics: Glycomics; N-glycan profiling.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,24:03
HLA-BB*35:01,44:03
HLA-CC*04:01,04:01
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Children:
CVCL_2N03 (WiDr/R)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-218; CLS=300377; ECACC=85111501; JCRB=IFO50043; JCRB=JCRB0224; KCLB=10218; PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,12
D2S133819,23
D3S135815,17
D5S81811,12
D7S82010
D8S117910 (CLS=300377)
10,16 (ATCC=CCL-218)
D13S31711 (CLS=300377; JCRB=JCRB0224)
11,12 (ATCC=CCL-218; ECACC=85111501; JCRB=IFO50043; KCLB=10218; PubMed=25926053)
D16S53911,12
D18S5113
D19S43314
D21S1129,30
FGA20,22
Penta D11,13
Penta E14,16
TH016,9
TPOX8,9
vWA17,19

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Provider; Altogen; Xenograft model; https://altogenlabs.com/xenograft-models/colon-cancer-xenograft/widr-xenograft-model/
Publications

PubMed=4203458
John C. Petricciani, Roslyn Elizabeth Wallace, Donald W. McCoy;
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Jorgen Fogh, William C. Wright, James D. Loveless;
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=90012; DOI=10.1007/BF02618407
Philip D. Noguchi, Roslyn Elizabeth Wallace, Joyce B. Johnson, Elisabeth M. Earley, Stephen James O'Brien, Soldano Ferrone, Michele A. Pellegrino, Julie B. Milstien, Charles F. Needy, Walter Browne, John C. Petricciani;
Characterization of the WiDr: a human colon carcinoma cell line.
In Vitro 15:401-408(1979)

CLPUB00271
Roslyn Elizabeth Wallace, Delores Lindh, Frederick E. Durr;
Studies on the development of resistance to mitoxantrone in human colon carcinoma cells in vitro.
Proc. Am. Assoc. Cancer Res. 23:195.767-195.767(1982)

PubMed=7118132; DOI=10.1007/BF02796478
John C. Petricciani, Paula Smith, Elisabeth M. Earley, Inessa S. Levenbook, Philip D. Noguchi;
Characteristics of seven clones of the WiDr human colon adenocarcinoma cell line.
In Vitro 18:492-494(1982)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3472642; DOI=10.1016/0165-4608(87)90267-6
Tchaw-Ren Chen, Douglas J. Drabkowski, Robert J. Hay, Marvin L. Macy, Ward Davis Peterson Jr.;
WiDr is a derivative of another colon adenocarcinoma cell line, HT-29.
Cancer Genet. Cytogenet. 27:125-134(1987)

PubMed=3335022
Michael C. Alley, Dominic A. Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty Jane Abbott, Joseph G. Mayo, Robert H. Shoemaker, Michael R. Boyd;
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=1657424; DOI=10.1007/BF00685821
Jean-Louis Fischel, Patricia Formento, Maryse Berlion, Jocelyne Berille, Jeannine Gioanni, Jean-Pierre Bizzari, Gerard Milano;
Sequence-dependent cytotoxic effects of the combination of a new nitrosourea, fotemustine, with 5-fluorouracil plus folinic acid.
Cancer Chemother. Pharmacol. 28:448-454(1991)

PubMed=1389533; DOI=10.1016/0959-8049(92)90031-V
Harald Lahm, Danuta Petral-Malec, Aysim Yilmaz-Ceyhan, Jurgen R. Fischer, Murielle Lorenzoni, Jean-Claude Givel, Nikolas Odartchenko;
Growth stimulation of a human colorectal carcinoma cell line by interleukin-1 and -6 and antagonistic effects of transforming growth factor beta 1.
Eur. J. Cancer 28A:1894-1899(1992)

PubMed=8464898; DOI=10.1073/pnas.90.7.2842; PMCID=PMC46192
Michael J. Browning, Peter Krausa, Andrew J. Rowan, David C. Bicknell, Julia Gwynaeth Bodmer, Walter Fred Bodmer;
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993)

PubMed=7874267; DOI=10.1007/BF02349278
Shigeo Ikuta, Fumio Itoh, Yuji Hinoda, Minoru Toyota, Yusuke Makiguchi, Kohzoh Imai, Akira Yachi;
Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma.
J. Gastroenterol. 29:727-732(1994)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9488600; DOI=10.1007/s002800050744
Jian-Guo Ma, Marc Maliepaard, Kees Nooter, Antonius W.M. Boersma, Jaap Verweij, Gerrit Stoter, Jan H.M. Schellens;
Synergistic cytotoxicity of cisplatin and topotecan or SN-38 in a panel of eight solid-tumor cell lines in vitro.
Cancer Chemother. Pharmacol. 41:307-316(1998)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ja-Lok Ku, Kyong-Ah Yoon, D.-Y. Kim, Jae-Gahb Park;
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=10956384; DOI=10.1002/1097-0215(20000915)87:6<771::AID-IJC2>3.0.CO;2-V
Harold H.J. Backus, Herbert Michael Pinedo, Dorine Wouters, Jose Manuel Padron, N. Molders, Clasina L. van der Wilt, Cornelis J. van Groeningen, Gerrit Jansen, Godefridus Johannes Peters;
Folate depletion increases sensitivity of solid tumor cell lines to 5-fluorouracil and antifolates.
Int. J. Cancer 87:771-778(2000)

PubMed=11414198; DOI=10.1007/s004320000207
Harald Lahm, Sabine Andre, Andreas Hoeflich, Jurgen R. Fischer, Bernard Sordat, Herbert Kaltner, Eckhard Wolf, Hans-Joachim Gabius;
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=16854228; DOI=10.1186/1476-4598-5-29; PMCID=PMC1550420
Eva Maria Bandres Elizalde, Elena Cubedo, Xabier Agirre, Raquel Malumbres, Ruth Zarate, Natalia Ramirez, Ana Abajo, Alfons Navarro, Isabel Moreno, Mariano Monzo, Jesus Garcia-Foncillas;
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29.1-29.10(2006)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514
Manickam Janakiraman, Efsevia Vakiani, Zhao-Shi Zeng, Christine A. Pratilas, Barry Stephen Taylor, Dhananjay Chitale, Ensar Halilovic, Manda Wilson, Kety Huberman, Julio Cezar Marques Ricarte Filho, Yogindra Persaud ...Show all 21 authors... , Douglas A. Levine, James Alexander Fagin, Suresh C. Jhanwar, John M. Mariadason, Alex Lash, Marc Ladanyi, Leonard B. Saltz, Adriana Heguy, Philip B. Paty, David B. Solit; Show fewer authors
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=23932154; DOI=10.1016/j.radonc.2013.06.032
Junius Salendo, Melanie Spitzner, Frank Kramer, Xin Zhang, Peter Jo, Hendrik A. Wolff, Julia Kitz, Silke Kaulfuss, Tim Beissbarth, Matthias Dobbelstein, Michael Ghadimi ...Show all 13 authors... , Marian Grade, Jochen Gaedcke; Show fewer authors
Identification of a microRNA expression signature for chemoradiosensitivity of colorectal cancer cells, involving miRNAs-320a, -224, -132 and let7g.
Radiother. Oncol. 108:451-457(2013)

PubMed=24042735; DOI=10.1038/oncsis.2013.35; PMCID=PMC3816225
Deeqa Ahmed, Peter W. Eide, Ina A. Eilertsen, Stine A. Danielsen, Mette Eknaes, Merete Hektoen, Guro Elisabeth Lind, Ragnhild Adelheid Lothe;
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:e71.1-e71.8(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Enzo Medico, Mariangela Russo, Gabriele Picco, Carlotta Cancelliere, Emanuele Valtorta, Giorgio Corti, Michela Buscarino, Claudio Isella, Simona Lamba, Barbara Martinoglio, Silvio Veronese ...Show all 19 authors... , Salvatore Siena, Andrea Sartore-Bianchi, Marco Beccuti, Marcella Mottolese, Michael Linnebacher, Francesca Cordero, Federica Di Nicolantonio, Alberto Bardelli; Show fewer authors
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457
Sarah Bazzocco, Higinio Dopeso, Fernando Carton-Garcia, Irati Macaya, Elena Andretta, Fiona Chionh, Paulo Rodrigues, Miriam Garrido, Hafid Alazzouzi, Rocio Nieto, Alex Sanchez ...Show all 15 authors... , Simo Schwartz Jr., Josipa Bilic, John M. Mariadason, Diego Arango; Show fewer authors
Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.
Clin. Cancer Res. 21:3695-3704(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235; PMCID=PMC4762531
Stephanie Holst, Anna J.M. Deuss, Gabi W. van Pelt, Sandra Johanna van Vliet, Juan J. Garcia-Vallejo, Carolien A.M. Koeleman, Andre M. Deelder, Wilma E. Mesker, Rob A.E.M. Tollenaar, Yoann Rombouts, Manfred Wuhrer;
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28683746; DOI=10.1186/s12943-017-0691-y; PMCID=PMC5498998
Kaja Christine Graue Berg, Peter W. Eide, Ina A. Eilertsen, Bjarne Johannessen, Jarle Bruun, Stine A. Danielsen, Merete Bjornslett, Leonardo Andres Meza-Zepeda, Mette Eknaes, Guro Elisabeth Lind, Ola Myklebost ...Show all 14 authors... , Rolf Inge Skotheim, Anita Sveen, Ragnhild Adelheid Lothe; Show fewer authors
Multi-omics of 34 colorectal cancer cell lines -- a resource for biomedical studies.
Mol. Cancer 16:116.1-116.16(2017)

Cross-references
Cell line collections (Providers) ATCC; CCL-218
BCRC; 60157
CLS; 300377
ECACC; 85111501
ICLC; HTL00003
JCRB; IFO50043
JCRB; JCRB0224
KCLB; 10218
NCI-DTP; WIDR
RCB; RCB1908 - Discontinued
TKG; TKG 0513
TKG; TKG 0516
Cell line databases/resources CLO; CLO_0009600
CLDB; cl4715
CLDB; cl4988
cancercelllines; CVCL_2760
Cell_Model_Passport; SIDM00152
ColonAtlas; WIDR
FCS-free; 180-2-342-3-16-3
IGRhCellID; WIDR
LINCS_HMS; 50049
LINCS_LDP; LCL-1169
Anatomy/cell type resources BTO; BTO_0001483
Biological sample resources BioSample; SAMN03151797
Chemistry resources ChEMBL-Cells; CHEMBL3307674
ChEMBL-Targets; CHEMBL614647
PharmacoDB; WIDR_1660_2019
PubChem_Cell_line; CVCL_2760
Encyclopedic resources Wikidata; Q54994056
Experimental variables resources EFO; EFO_0002389
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
GEO; GSM784020
GEO; GSM827327
GEO; GSM1006242
GEO; GSM1006243
GEO; GSM1006244
GEO; GSM1374987
GEO; GSM1374988
GEO; GSM1448091
GEO; GSM2550022
Metabolomic databases MetaboLights; MTBLS227
Polymorphism and mutation databases Cosmic; 738928
Cosmic; 887216
Cosmic; 897740
Cosmic; 905002
Cosmic; 1066213
Cosmic; 1122336
Cosmic; 1132695
Cosmic; 1184091
Cosmic; 1187326
Cosmic; 1218881
Cosmic; 1466826
Cosmic; 1479615
Cosmic; 1609498
Cosmic; 1676736
Cosmic; 2651871
Cosmic; 2664048
Cosmic; 2668255
Cosmic; 2760078
IARC_TP53; 21
Progenetix; CVCL_2760
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001002554
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43