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Cellosaurus SKW-3 (CVCL_2197)

[Text version]
Cell line name SKW-3
Synonyms SKW3
Accession CVCL_2197
Resource Identification Initiative To cite this cell line use: SKW-3 (RRID:CVCL_2197)
Comments Problematic cell line: Contaminated. Shown to be a KE-37 derivative (PubMed=20143388). Originally thought to originate from the peripheral blood of a 61 year old male patient with T cell chronic lymphocytic leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00346.
Population: Caucasian.
Doubling time: ~30-40 hours (DSMZ=ACC-53); ~30 hours (CLS=300343).
Sequence variations
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Gln2459Ter (c.7375C>T); Zygosity=Heterozygous (PubMed=15472075).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9585; PTCH1; Simple; p.Arg571Gln (c.1712G>A); ClinVar=VCV000819886; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Gly310Glu (c.929G>A); ClinVar=VCV000237678; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro153Alafs*28 (c.455dupC) (c.455_456insC) (p.P152fs) (c.459_460insC); ClinVar=VCV000182958; Zygosity=Heterozygous (from parent cell line).
Disease Adult T acute lymphoblastic leukemia (NCIt: C9142)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1327 (KE-37)
Children:
CVCL_J125 (SK-beta2.7)CVCL_4V58 (SKW3/Clo)
Sex of cell Male
Age at sampling 27Y
Category Cancer cell line
STR profile Source(s): CLS=300343; DSMZ=ACC-53; RCB=RCB1168; TKG=TKG 0247

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165615.3,16
D2S133819,25
D3S135815,18
D5S81812,13
D6S104318,21
D7S8208,12
D8S117911,14
D12S39119,22
D13S3178,12
D16S53911,12
D18S5113,18
D19S43313,15
D21S1128,29,30 (CLS=300343)
28,30 (DSMZ=ACC-53)
FGA24,25
Penta D11,15
Penta E5,14
TH016,9.3
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; T-ALL cell lines db; -; https://humantallcelllines.wordpress.com/comprehensivetable/skw-3-ke-37/
Publications

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 Carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=8127147
Mats Heyman, Dan Grander, Karen Brondum-Nielsen, Britta Cederblad, Yie Liu, Bo Xu, Stefan Einhorn;
Interferon system defects in malignant T-cells.
Leukemia 8:425-434(1994)

PubMed=7849311; DOI=10.1182/blood.V85.4.893.bloodjournal854893
Gail Stranks, Susan E. Height, Paul Mitchell, Dalal Mouneimne Jadayel, Martin A.R. Yuille, Corinne F.M. De Lord, Robyn D. Clutterbuck, Jennie G. Treleaven, Ray Leonard Powles, Elisabeth Nacheva, David G. Oscier ...Show all 17 authors... , Abraham Karpas, Gilbert Marcel Lenoir, Stephen D. Smith, John L. Millar, Daniel Catovsky, Martin John Swinnerton Dyer; Show fewer authors
Deletions and rearrangement of CDKN2 in lymphoid malignancy.
Blood 85:893-901(1995)

PubMed=8641406; DOI=10.1111/j.1600-0609.1996.tb00721.x
Laura Borgonovo-Brandter, Mats Heyman, Omid Rasool, Y. Liu, Dan Grander, Stefan Einhorn;
p16INK4/p15INK4B gene inactivation is a frequent event in malignant T-cell lines.
Eur. J. Haematol. 56:313-318(1996)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588
Noriko Hosoya, Akira Hangaishi, Seishi Ogawa, Kiyoshi Miyagawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai;
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Renate Burger, Thomas E. Hansen-Hagge, Hans Gunther Drexler, Martin Gramatzki;
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=15472075; DOI=10.1126/science.1102160
Andrew P. Weng, Adolfo A. Ferrando, Woojoong Lee, John P. Morris 4th, Lewis B. Silverman, Cheryll Sanchez-Irizarry, Stephen Charles Blacklow, Alfred Thomas Look, Jon Christopher Aster;
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections (Providers) CLS; 300343
DSMZ; ACC-53
NCBI_Iran; C182
RCB; RCB1168
TKG; TKG 0247
Cell line databases/resources CLO; CLO_0009068
CLO; CLO_0051007
CLDB; cl4362
cancercelllines; CVCL_2197
DSMZCellDive; ACC-53
Anatomy/cell type resources BTO; BTO_0006057
Biological sample resources BioSample; SAMN03151818
BioSample; SAMN03473523
Encyclopedic resources Wikidata; Q108821313
Gene expression databases FANTOM5_SSTAR; 10416-106C2
Polymorphism and mutation databases Cosmic; 913412
Cosmic; 919156
Cosmic; 933546
Cosmic; 1524820
Cosmic; 1641385
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number33