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Cellosaurus SKW-3 (CVCL_2197)

[Text version]

Cell line name SKW-3
Synonyms SKW3
Accession CVCL_2197
Resource Identification Initiative To cite this cell line use: SKW-3 (RRID:CVCL_2197)
Comments Problematic cell line: Contaminated. Shown to be a KE-37 derivative (PubMed=20143388). Originally thought to originate from the peripheral blood of a 61 year old male patient with T cell chronic lymphocytic leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00346.
Population: Caucasian.
Doubling time: ~30-40 hours (DSMZ); ~30 hours (CLS).
Sequence variations
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Gln2459Ter (c.7375C>T); Zygosity=Heterozygous (PubMed=15472075).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9585; PTCH1; Simple; p.Arg571Gln (c.1712G>A); ClinVar=VCV000819886; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9884; RB1; Simple; p.Gly310Glu (c.929G>A); ClinVar=VCV000237678; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Pro153Alafs*28 (c.455dupC) (c.455_456insC) (p.P152fs) (c.459_460insC); ClinVar=VCV000182958; Zygosity=Heterozygous (from parent cell line).
Disease Adult T acute lymphoblastic leukemia (NCIt: C9142)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1327 (KE-37)
Children:
CVCL_J125 (SK-beta2.7 )CVCL_4V58 (SKW3/Clo )
Sex of cell Male
Age at sampling 27Y
Category Cancer cell line
STR profile Source(s): CLS; DSMZ; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165615.3,16
D2S133819,25
D3S135815,18
D5S81812,13
D6S104318,21
D7S8208,12
D8S117911,14
D12S39119,22
D13S3178,12
D16S53911,12
D18S5113,18
D19S43313,15
D21S1128,29,30 (CLS)
28,30 (DSMZ)
FGA24,25
Penta D11,15
Penta E5,14
TH016,9.3
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Web pages http://iclac.org/wp-content/uploads/Cross-Contaminations_v11_distribution.xlsx
Publications

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=8127147
Heyman M., Grander D., Brondum-Nielsen K., Cederblad B., Liu Y., Xu B., Einhorn S.
Interferon system defects in malignant T-cells.
Leukemia 8:425-434(1994)

PubMed=7849311; DOI=10.1182/blood.V85.4.893.bloodjournal854893
Stranks G., Height S.E., Mitchell P., Jadayel D.M., Yuille M.A.R., De Lord C., Clutterbuck R.D., Treleaven J.G., Powles R.L., Nacheva E., Oscier D.G., Karpas A., Lenoir G.M., Smith S.D., Millar J.L., Catovsky D., Dyer M.J.S.
Deletions and rearrangement of CDKN2 in lymphoid malignancy.
Blood 85:893-901(1995)

PubMed=8641406; DOI=10.1111/j.1600-0609.1996.tb00721.x
Borgonovo Brandter L., Heyman M., Rasool O., Liu Y., Grander D., Einhorn S.
p16INK4/p15INK4B gene inactivation is a frequent event in malignant T-cell lines.
Eur. J. Haematol. 56:313-318(1996)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x
Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Burger R., Hansen-Hagge T.E., Drexler H.G., Gramatzki M.
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. IV, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections CLS; 300343
DSMZ; ACC-53
NCBI_Iran; C182
RCB; RCB1168
TKG; TKG 0247
Cell line databases/resources CLO; CLO_0009068
CLO; CLO_0051007
CLDB; cl4362
DSMZCellDive; ACC-53
Anatomy/cell type resources BTO; BTO:0006057
Biological sample resources BioSample; SAMN03151818
BioSample; SAMN03473523
Encyclopedic resources Wikidata; Q108821313
Polymorphism and mutation databases Cosmic; 913412
Cosmic; 919156
Cosmic; 933546
Cosmic; 1524820
Cosmic; 1641385
Entry history
Entry creation04-Apr-2012
Last entry update23-Jun-2022
Version number27