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Cellosaurus MT-3 [Human contaminated breast cancer] (CVCL_2129)

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Cell line name MT-3 [Human contaminated breast cancer]
Synonyms MT3
Accession CVCL_2129
Resource Identification Initiative To cite this cell line use: MT-3 [Human contaminated breast cancer] (RRID:CVCL_2129)
Comments Problematic cell line: Contaminated. Shown to be a LS174T derivative (PubMed=25877200; DSMZ). Originally thought to originate from a breast carcinoma.
Part of: MD Anderson Cell Lines Project.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00506.
Population: Caucasian.
Doubling time: 37.1 +- 8.1 hours (PubMed=9150904); ~30 hours (DSMZ=ACC-403).
Microsatellite instability: Instable (MSI) (PubMed=12661003; PubMed=15677628).
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.
Sequence variations
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45Phe (c.134C>T); ClinVar=VCV000017588; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Unspecified (from parent cell line).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,02:01
HLA-BB*07:17,15:50
HLA-CC*04:01,06:02
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1384 (LS174T)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-403; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10,13
D2S133818,22
D3S135815,16
D5S81812 (PubMed=25877200)
12,17 (DSMZ=ACC-403)
D7S82010.3,11,12
D8S117912,15 (PubMed=25877200)
13,15 (DSMZ=ACC-403)
D13S31710,11
D16S53911,13
D18S5110,12
D19S43313,14
D21S1129,31
FGA20,21
Penta D9,10
Penta E13,15 (PubMed=25877200)
13,15,17,21 (DSMZ=ACC-403)
TH016,7
TPOX8,9
vWA13,14,16,17 (DSMZ=ACC-403)
14,17 (PubMed=25877200)

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; MCLP; -; https://tcpaportal.org/mclp/
Info; Pawefish; -; https://www.cellosaurus.org/pawefish/BreastCellLineDescriptions/MT-3.html
Publications

PubMed=1446057; DOI=10.1007/BF01831480
Helga Naundorf, E.C. Rewasowa, Iduna Fichtner, Britta Buttner, Michael Becker, Max Gorlich;
Characterization of two human mammary carcinomas, MT-1 and MT-3, suitable for in vivo testing of ether lipids and their derivatives.
Breast Cancer Res. Treat. 23:87-95(1992)

PubMed=9150904; DOI=10.1023/A:1005756632293
Richard J. Hambly, John A. Double, Michael J. Thompson, Michael C. Bibby;
Establishment and characterisation of new cell lines from human breast tumours initially established as tumour xenografts in NMRI nude mice.
Breast Cancer Res. Treat. 43:247-258(1997)

PubMed=11044355; DOI=10.1054/bjoc.2000.1458; PMCID=PMC2408781
Jeffrey M. Davidson, Kylie Louise Gorringe, Suet-Feung Chin, Beatrice Orsetti, C. Besret, Celine Courtay-Cahen, Ian Roberts, Charles Theillet, Carlos Caldas, Paul A.W. Edwards;
Molecular cytogenetic analysis of breast cancer cell lines.
Br. J. Cancer 83:1309-1317(2000)

PubMed=12661003; DOI=10.1002/gcc.10196
Susanne Seitz, Peter Wassmuth, Jens Plaschke, Hans Konrad Schackert, Uwe Karsten, Mauro F. Santibanez-Koref, Peter Michael Schlag, Siegfried Scherneck;
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=12800145; DOI=10.1002/gcc.10218
Jose Adelaide, Huai-En Huang, Anne Murati, Amber E. Alsop, Beatrice Orsetti, Marie-Joelle Mozziconacci, Cornel Popovici, Christophe Ginestier, Anne Letessier, Celine Basset, Celine Courtay-Cahen ...Show all 16 authors... , Jocelyne Jacquemier, Charles Theillet, Daniel Birnbaum, Paul A.W. Edwards, Max Chaffanet; Show fewer authors
A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.
Genes Chromosomes Cancer 37:333-345(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Kylie Louise Gorringe, Suet-Feung Chin, Paul D.P. Pharoah, Joanne M. Staines, Carla Oliveira, Paul A.W. Edwards, Carlos Caldas;
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590
Anneleen Daemen, Obi L. Griffith, Laura M. Heiser, Nicholas J. Wang, Oana M. Enache, Zachary Sanborn, Francois Pepin, Steffen Durinck, James E. Korkola, Malachi Griffith, Joe S. Hur ...Show all 27 authors... , Nam Huh, Jongsuk Chung, Leslie Cope, Mary Jo Fackler, Christopher Benedict Umbricht, Saraswati Sukumar, Pankaj Seth, Vikas P. Sukhatme, Lakshmi R. Jakkula, Yi-Ling Lu, Gordon B. Mills, Raymond J. Cho, Eric A. Collisson, Laura J. van 't Veer, Paul T. Spellman, Joe W. Gray; Show fewer authors
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections (Providers) DSMZ; ACC-403 - Discontinued
Cell line databases/resources CLO; CLO_0007890
cancercelllines; CVCL_2129
DSMZCellDive; ACC-403
IGRhCellID; MT3
LINCS_HMS; 50031
LINCS_LDP; LCL-1473
Biological sample resources BioSample; SAMN03472975
BioSample; SAMN03473582
BioSample; SAMN06481118
Chemistry resources PharmacoDB; MT3_969_2019
Encyclopedic resources Wikidata; Q54906937
Experimental variables resources EFO; EFO_0002241
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
GEO; GSM115102
GEO; GSM217592
GEO; GSM412073
GEO; GSM412084
GEO; GSM412093
GEO; GSM412098
GEO; GSM412106
GEO; GSM412110
GEO; GSM459700
GEO; GSM474280
GEO; GSM474281
GEO; GSM476484
GEO; GSM476485
GEO; GSM478311
GEO; GSM478312
GEO; GSM478314
GEO; GSM478315
GEO; GSM478320
GEO; GSM478321
GEO; GSM783977
GEO; GSM844610
GEO; GSM847419
GEO; GSM847498
GEO; GSM1172986
Polymorphism and mutation databases Progenetix; CVCL_2129
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number36