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Cellosaurus WISH (CVCL_1909)

[Text version]
Cell line name WISH
Synonyms Wish; WISH (HeLa derivative); Wistar Institute, Susan Hayflick
Accession CVCL_1909
Resource Identification Initiative To cite this cell line use: WISH (RRID:CVCL_1909)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=6451928; PubMed=20143388). Originally thought to originate from cells of the amniotic sac of Susan Hayflick, the daughter of Leonard Hayflick.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00013.
Population: African American.
Virology: Used for differentiation of virulent and non virulent strains of measles virus (ECACC=88102403; IZSLER=BS CL 92).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_8345 (ED(27))CVCL_W635 (HES [Human contaminated endometrial epithelial])CVCL_A8RR (WISH-Mx2/EGFP)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC=CCL-25; CCRID=1101HUM-PUMC000088; ECACC=88102403; JCRB=IFO50004; KCLB=10025

D7S8208,12 (ATCC=CCL-25; CCRID=1101HUM-PUMC000088; JCRB=IFO50004; KCLB=10025)
12 (ECACC=88102403)
D13S31713.3 (ATCC=CCL-25; CCRID=1101HUM-PUMC000088; JCRB=IFO50004; KCLB=10025)
14 (ECACC=88102403)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=WISH

PubMed=13712490; DOI=10.1016/0014-4827(61)90059-3
Hayflick L.
The establishment of a line (WISH) of human amnion cells in continuous cultivation.
Exp. Cell Res. 23:14-20(1961)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=6451928; DOI=10.1126/science.6451928
Nelson-Rees W.A., Daniels D.W., Flandermeyer R.R.
Cross-contamination of cells in culture.
Science 212:446-452(1981)

Gold M.
A conspiracy of cells. One woman's immortal legacy and the medical scandal it caused.
(In) ISBN 9780887060991; pp.1-171; State University of New York Press; Albany (1986)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=22549076; DOI=10.1016/j.jim.2012.04.010
Burgi M., Prieto C., Etcheverrigaray M., Kratje R., Oggero M., Bollati-Fogolin M.
WISH cell line: from the antiviral system to a novel reporter gene assay to test the potency of human IFN-alpha and IFN-beta.
J. Immunol. Methods 381:70-74(2012)

PubMed=23925245; DOI=10.1038/nature12064
Adey A.C., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

Cell line collections (Providers) ATCC; CCL-25
ATCC; CRL-7727 - Discontinued
ECACC; 88102403
JCRB; IFO50004
KCLB; 10025
Cell line databases/resources CLO; CLO_0009609
CLDB; cl4720
CLDB; cl4721
CLDB; cl4723
CLDB; cl4724
CCRID; 1101HUM-PUMC000088
Anatomy/cell type resources BTO; BTO:0000069
Biological sample resources BioSample; SAMN03151764
Chemistry resources ChEMBL-Cells; CHEMBL3308013
ChEMBL-Targets; CHEMBL612811
PubChem_Cell_line; CVCL_1909
Encyclopedic resources Wikidata; Q54994114
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number32