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Cellosaurus HEp-2 (CVCL_1906)

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Cell line name HEp-2
Synonyms Hep-2; HEP-2; Hep2 (HeLa derivative); HEp-2/HeLa; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2
Accession CVCL_1906
Resource Identification Initiative To cite this cell line use: HEp-2 (RRID:CVCL_1906)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to originate from a 56 year old male patient with a laryngeal carcinoma.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00007.
Population: African American.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Ubiquitination analysis by proteomics.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
HLA typing Source: PubMed=26589293
Class I
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_WH94 (Ban)CVCL_W888 (DRHEp2)CVCL_2817 (HEp-2 clone 2B)
CVCL_2940 (HEp-2C)CVCL_RX04 (HEp2-Mx2/EGFP L1D7)CVCL_RX05 (HEp2-Mx2/EGFP L1G5)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): AddexBio=C0050001/4983; ATCC=CCL-23; CCRID; CLS=300397; KCLB=10023; PubMed=11416159; PubMed=17254797; RCB=RCB1889; TKG=TKG 0403

D13S31712 (RCB=RCB1889)
12,13.3 (AddexBio=C0050001/4983; ATCC=CCL-23; CCRID; CLS=300397; KCLB=10023; TKG=TKG 0403)
12,14 (PubMed=17254797)
D18S5115 (PubMed=17254797)
16 (ATCC=CCL-23; CCRID; CLS=300397; PubMed=11416159)
D21S1126,27 (PubMed=17254797)
27,28 (ATCC=CCL-23; CCRID; CLS=300397; PubMed=11416159)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-204.html

Toolan H.W.
Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3 and H.Emb.Rh. No. 1.
Cancer Res. 14:660-666(1954)

Moore A.E., Sabachewsky L., Toolan H.W.
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res. 15:598-602(1955)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)

Ogura H., Yoshinouchi M., Kudo T., Imura M., Fujiwara T., Yabe Y.
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

Tsuboi I., Taguchi M., Kenmotsu M., Ban F., Machida K., Inagaki K., Ishi A., Tazawa T.
A new substrain derived from HEp-2 for the detection of anti-nuclear antibodies.
Tissue Cult. Res. Commun. 17:95-100(1998)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Azari S., Ahmadi N., Jeddi-Tehrani M., Shokri F.
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=19450234; DOI=10.2144/000113089
Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064
Adey A.C., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A.
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=29483928; DOI=10.1186/s11658-017-0067-8
Li G.-D., Hu X.-L., Sun L., Li X., Li J.-F., Li T.-L., Zhang X.-H.
C-fos upregulates P-glycoprotein, contributing to the development of multidrug resistance in HEp-2 laryngeal cancer cells with VCR-induced resistance.
Cell. Mol. Biol. Lett. 23:6.1-6.12(2018)

PubMed=29967540; DOI=10.1038/s41594-018-0084-y
Akimov V., Barrio-Hernandez I., Hansen S.V.F., Hallenborg P., Pedersen A.-K., Bekker-Jensen D.B., Puglia M., Christensen S.D.K., Vanselow J.T., Nielsen M.M., Kratchmarova I., Kelstrup C.D., Olsen J.V., Blagoev B.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.
Nat. Struct. Mol. Biol. 25:631-640(2018)

Gorphe P.
A comprehensive review of Hep-2 cell line in translational research for laryngeal cancer.
Am. J. Cancer Res. 9:644-649(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

PubMed=34024160; DOI=10.2144/btn-2020-0162
Fusi M., Dotti S.
Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth.
BioTechniques 70:319-326(2021)

PubMed=34697958; DOI=10.1177/24725552211051963
Korch C.T., Capes-Davis A.
The extensive and expensive impacts of HEp-2 [HeLa], intestine 407 [HeLa], and other false cell lines in journal publications.
SLAS Discov. 26:1268-1279(2021)

Cell line collections (Providers) AddexBio; C0050001/4983
BCRC; 60042 - Discontinued
BCRJ; 0101
CLS; 300397
ECACC; 86030501
KCLB; 10023
NCBI_Iran; C144
RCB; RCB1889
TKG; TKG 0403
Cell line databases/resources CLO; CLO_0003706
CLO; CLO_0003707
CLO; CLO_0050927
CLDB; cl1646
CLDB; cl1647
CLDB; cl1649
CLDB; cl1650
CLDB; cl1653
CLDB; cl1654
CLDB; cl1655
CCRID; 1101HUM-PUMC000068
Cell_Model_Passport; SIDM01208
TOKU-E; 1442
Anatomy/cell type resources BTO; BTO:0000976
Biological sample resources BioSample; SAMN03151706
Chemistry resources ChEMBL-Cells; CHEMBL3307694
ChEMBL-Targets; CHEMBL614735
PharmacoDB; HEp2_540_2019
PubChem_Cell_line; CVCL_1906
Encyclopedic resources Wikidata; Q16554737
Experimental variables resources EFO; EFO_0006438
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM1374529
GEO; GSM1374530
Polymorphism and mutation databases Cosmic; 1339928
Cosmic; 1530739
Cosmic; 1969495
Cosmic; 2301559
Proteomic databases PRIDE; PXD006201
PRIDE; PXD013072
PRIDE; PXD014913
PRIDE; PXD031726
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number41