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Cellosaurus HEp-2 (CVCL_1906)

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Cell line name HEp-2
Synonyms Hep-2; HEP-2; Hep2 (HeLa derivative); HEp-2/HeLa; Hep 2; Hep2; HEp2; HEP2; H.Ep.-2; H.Ep. #2; H.Ep. No. 2; Hep II; Human Epidermoid carcinoma #2; Human Epithelioma-2
Accession CVCL_1906
Resource Identification Initiative To cite this cell line use: HEp-2 (RRID:CVCL_1906)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to originate from a 56 year old male patient with a laryngeal carcinoma.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00007.
Population: African American.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genomics; Whole genome sequencing; Low read coverage.
Omics: Proteomics; PTM; Ubiquitination.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
HLA typing Source: PubMed=26589293
Class I
HLA-AA*68:02,68:02
HLA-BB*15:03,15:03
HLA-CC*12:03,12:03
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_WH94 (Ban)CVCL_W888 (DRHEp2)CVCL_2817 (HEp-2 clone 2B)
CVCL_GZ00 (Hep-2 TPFR)CVCL_A8JP (HEp-2-NS1)CVCL_B7H7 (HEp-2/VCR)
CVCL_2940 (HEp-2C)CVCL_RX04 (HEp2-Mx2/EGFP L1D7)CVCL_RX05 (HEp2-Mx2/EGFP L1G5)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): AddexBio=C0050001/4983; ATCC=CCL-23; CCRID; CLS=300397; KCLB=10023; PubMed=11416159; PubMed=17254797; RCB=RCB1889; TKG=TKG 0403

Markers:
AmelogeninX
CSF1PO9,10
D2S133817
D3S135815,18
D5S81811,12
D7S8208,12
D8S117912,13
D13S31712 (RCB=RCB1889)
12,13.3 (AddexBio=C0050001/4983; ATCC=CCL-23; CCRID; CLS=300397; KCLB=10023; TKG=TKG 0403)
12,14 (PubMed=17254797)
D16S5399,10
D18S5115 (PubMed=17254797)
16 (ATCC=CCL-23; CCRID; CLS=300397; PubMed=11416159)
D19S43313,14
D21S1126,27 (PubMed=17254797)
27,28 (ATCC=CCL-23; CCRID; CLS=300397; PubMed=11416159)
FGA18,21
Penta D8,15
Penta E7,17
TH017
TPOX8,12
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-204.html
Info; Wikipedia; -; https://es.wikipedia.org/wiki/C%C3%A9lulas_HEp-2
Provider; Vircell; -; https://www.vircell.com/en/products/hep-2-cell-line
Publications

PubMed=13209540
Helene Wallace Toolan;
Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1; H.Ep. No. 1; H.Ep. No. 2; H.Ep. No. 3 and H.Emb.Rh. No. 1.
Cancer Res. 14:660-666(1954)

PubMed=13261081
Alice E. Moore, Lillian Sabachewsky, Helene Wallace Toolan;
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res. 15:598-602(1955)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

PubMed=4864103
Stanley Michael Gartler;
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Stanley Michael Gartler;
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Ward Davis Peterson Jr., Cyril S. Stulberg, N.K. Swanborg, Andria Rasile Robinson;
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4203458
John C. Petricciani, Roslyn Elizabeth Wallace, Donald W. McCoy;
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

DOI=10.1007/978-1-4757-1647-4_13
June Lee Biedler;
Chromosome abnormalities in human tumor cells in culture.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.359-394; Springer; New York; USA (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Walter Anthony Nelson-Rees, Robert R. Flandermeyer;
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=566722; DOI=10.1007/BF02616110
Kantharajapura Subbegowda Lavappa;
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3180844; DOI=10.1159/000132579
Tchaw-Ren Chen;
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)

PubMed=8397027
Hajime Ogura, Mitsuo Yoshinouchi, Takafumi Kudo, Makoto Imura, Tazuko Fujiwara, Yoshiro Yabe;
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

DOI=10.11418/jtca1981.17.3_95
Isao Tsuboi, Michihiro Taguchi, Masashi Kenmotsu, Fumihiko Ban, Kunimitu Machida, Kouichi Inagaki, Akira Ishi, Tadashi Tazawa;
A new substrain derived from HEp-2 for the detection of anti-nuclear antibodies.
Tissue Cult. Res. Commun. 17:95-100(1998)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Shahram Azari, Nahid Ahmadi, Mahmood Jeddi-Tehrani, Fazel Shokri;
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=19450234; DOI=10.2144/000113089; PMCID=PMC2696096
Raheleh Rahbari, Tom Sheahan, Vasileios Modes, Pam Collier, Catriona M. Macfarlane, Richard M. Badge;
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Axel Karger, Barbara Bettin, Matthias Lenk, Thomas Christoph Mettenleiter;
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064; PMCID=PMC3740412
Andrew C. Adey, Joshua N. Burton, Jacob O. Kitzman, Joseph B. Hiatt, Alexandra P. Lewis, Beth K. Martin, Ruo-Lan Qiu, Choli Lee, Jay Shendure;
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

CLPUB00484
Zhi-Hong Xu, Wen-Wen Sun, Jun-Biao Hang, Bei-Li Gao, Jia-An Hu;
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=29483928; DOI=10.1186/s11658-017-0067-8; PMCID=PMC5819209
Guo-Dong Li, Xiao-Ling Hu, Lu Sun, Xin Li, Jian-Feng Li, Tong-Li Li, Xiao-Hui Zhang;
C-fos upregulates P-glycoprotein, contributing to the development of multidrug resistance in HEp-2 laryngeal cancer cells with VCR-induced resistance.
Cell. Mol. Biol. Lett. 23:6.1-6.12(2018)

PubMed=29967540; DOI=10.1038/s41594-018-0084-y
Vyacheslav Akimov, Inigo Barrio-Hernandez, Sten V.F. Hansen, Philip Hallenborg, Anna-Kathrine Pedersen, Dorte Breinholdt Bekker-Jensen, Michele Puglia, Stine D.K. Christensen, Jens T. Vanselow, Mogens M. Nielsen, Irina Kratchmarova ...Show all 14 authors... , Christian Dahl Kelstrup, Jesper Velgaard Olsen, Blagoy Blagoev; Show fewer authors
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.
Nat. Struct. Mol. Biol. 25:631-640(2018)

PubMed=31105993; PMCID=PMC6511639
Philippe Gorphe;
A comprehensive review of Hep-2 cell line in translational research for laryngeal cancer.
Am. J. Cancer Res. 9:644-649(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

PubMed=34024160; DOI=10.2144/btn-2020-0162
Mara Fusi, Silvia Dotti;
Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth.
BioTechniques 70:319-326(2021)

PubMed=34697958; DOI=10.1177/24725552211051963
Christopher T. Korch, Amanda Capes-Davis;
The extensive and expensive impacts of HEp-2 [HeLa], intestine 407 [HeLa], and other false cell lines in journal publications.
SLAS Discov. 26:1268-1279(2021)

Cross-references
Cell line collections (Providers) AddexBio; C0050001/4983
ATCC; CCL-23
BCRC; 60042 - Discontinued
BCRJ; 0101
CCLV; CCLV-RIE 0141
CCTCC; GDC0004
CLS; 300397
ECACC; 86030501
IZSLER; BS TCL 23
KCLB; 10023
NCBI_Iran; C144
RCB; RCB1889
TKG; TKG 0403
Cell line databases/resources CLO; CLO_0003706
CLO; CLO_0003707
CLO; CLO_0050927
CLDB; cl1646
CLDB; cl1647
CLDB; cl1649
CLDB; cl1650
CLDB; cl1653
CLDB; cl1654
CLDB; cl1655
CCRID; 1101HUM-PUMC000068
CCRID; 4201PAT-CCTCC00214
Cell_Model_Passport; SIDM01208
TOKU-E; 1442
Anatomy/cell type resources BTO; BTO_0000976
Biological sample resources BioSample; SAMN03151706
Chemistry resources ChEMBL-Cells; CHEMBL3307694
ChEMBL-Targets; CHEMBL614735
PharmacoDB; HEp2_540_2019
PubChem_Cell_line; CVCL_1906
Encyclopedic resources Wikidata; Q16554737
Experimental variables resources EFO; EFO_0006438
Gene expression databases ArrayExpress; E-MTAB-2706
FANTOM5_SSTAR; 11890-125D9
FANTOM5_SSTAR; 11891-125E1
FANTOM5_SSTAR; 11892-125E2
FANTOM5_SSTAR; 11894-125E4
FANTOM5_SSTAR; 11895-125E5
FANTOM5_SSTAR; 11896-125E6
FANTOM5_SSTAR; 11898-125E8
FANTOM5_SSTAR; 11899-125E9
FANTOM5_SSTAR; 11900-125F1
GEO; GSM1374529
GEO; GSM1374530
Polymorphism and mutation databases Cosmic; 1339928
Cosmic; 1530739
Cosmic; 1969495
Cosmic; 2301559
Proteomic databases PRIDE; PXD006201
PRIDE; PXD013072
PRIDE; PXD014913
PRIDE; PXD031726
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43