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Cellosaurus CCRF-HSB-2 (CVCL_1859)

[Text version]
Cell line name CCRF-HSB-2
Synonyms CCRF HSB-2; CCRF-HSB2; HSB-2; HSB.2; HSB2; H-SB2; SB-2; HSB; H.S.B.; GM04155
Accession CVCL_1859
Secondary accession CVCL_5938
Resource Identification Initiative To cite this cell line use: CCRF-HSB-2 (RRID:CVCL_1859)
Comments Problematic cell line: Partially contaminated. The stock formerly distributed by CLS under catalog number 300214 was contaminated. Compared to the STR values obtained by other distributors it differed from having: Amelogenin: X; CSF1PO: 8,12; D16S539: 9,13,14; D18S51: 9,13,14; D21S11: 28,29; D7S820: 10,14; D8S1179: 9,15; FGA: 22,23,24; Penta D: 9; Penta E: 6,13 and TH01: 8,10.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Characteristics: Established by serial transplantation into newborn Syrian hamsters.
Doubling time: 40 hours (PubMed=17117183); ~25 hours (DSMZ=ACC-435); 24 hours (Note=Lot 042091) (JCRB=JCRB0031).
Microsatellite instability: Instable (MSI) (PubMed=10739008; PubMed=11226526).
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; RNAseq.
Misspelling: CCRF-HBS-2; NCBI_Iran=C530.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; HGNC:10879; STIL + HGNC; HGNC:11556; TAL1; Name(s)=STIL-TAL1, SIL-TAL1, SIL-SCL (PubMed=2255914).
  • Mutation; HGNC; HGNC:3430; ERBB2; Simple; p.Pro1175Arg (c.3524C>G); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:3690; FGFR3; Simple; p.Tyr226His (c.676T>C); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:3690; FGFR3; Simple; p.Arg621His (c.1862G>A); ClinVar=VCV000016355; Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1600Pro (c.4799T>C); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Cys (c.34G>T); ClinVar=VCV000040468; Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:8979; PIK3R1; Simple; p.Thr454Ile (c.1361C>T); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:8979; PIK3R1; Simple; p.Gln455Lys (c.1363C>A); Zygosity=Unspecified (PubMed=22675565).
  • Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (PubMed=2144611).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A0DR (HSB.2-A7-D2)CVCL_A0DS (HSB.2-A7-D9)CVCL_A0DT (HSB.2-C5-B2)
CVCL_1Y12 (HSB2/GS)
Originate from same individual CVCL_1860 ! CCRF-SB
Sex of cell Male
Age at sampling 11Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-120.1; DSMZ=ACC-435; ECACC=85112801; JCRB=JCRB0031; KCLB=10120.1; RCB=RCB0016

Markers:
AmelogeninX,Y
CSF1PO10,11 (DSMZ=ACC-435)
10,12 (ATCC=CCL-120.1; ECACC=85112801; JCRB=JCRB0031; KCLB=10120.1; RCB=RCB0016)
D2S133817,18
D3S135814,16
D5S81811,12
D7S82011,12
D8S117910,15
D13S31710,12
D16S5399,12 (DSMZ=ACC-435)
9,13 (ATCC=CCL-120.1; ECACC=85112801; JCRB=JCRB0031; RCB=RCB0016)
D18S519,14
D19S43314,20
D21S1127,28
FGA24,25
Penta D9,12
Penta E8,13
TH019,9.3 (KCLB=10120.1)
9,10 (ATCC=CCL-120.1; DSMZ=ACC-435; ECACC=85112801; JCRB=JCRB0031; RCB=RCB0016)
TPOX8
vWA18,19 (ATCC=CCL-120.1; DSMZ=ACC-435; ECACC=85112801; JCRB=JCRB0031; KCLB=10120.1)
18,19,20 (RCB=RCB0016)

Run an STR similarity search on this cell line
Web pages Info; T-ALL cell lines db; -; https://humantallcelllines.wordpress.com/comprehensivetable/hsb-2/
Provider; HHV-6 Foundation; -; https://hhv-6foundation.org/wp-content/uploads/2016/11/HSB-2-Cells-data-sheet.pdf
Publications

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4991454; DOI=10.1016/0014-4827(79)90504-4
Richard A. Adams, Lillian Pothier, Arlene Flowers, Herbert Lazarus, Sidney Farber, George E. Foley;
The question of stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia.
Exp. Cell Res. 62:5-10(1970)

PubMed=4363409; DOI=10.1084/jem.139.5.1070; PMCID=PMC2139653
Joseph Kaplan, Thomas C. Shope, Ward Davis Peterson Jr.;
Epstein-Barr virus-negative human malignant T-cell lines.
J. Exp. Med. 139:1070-1076(1974)

PubMed=4523807; DOI=10.1007/BF02616012
Herbert Lazarus, Elizabeth Ferguson Barell, Selma O. Oppenheim, Awtar Krishan;
Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood.
In Vitro 9:303-310(1974)

PubMed=168255; DOI=10.4049/jimmunol.115.1.243
Lindsey M. Hutt, Yung-Tsun Huang, Harry Emerson Dascomb, Joseph S. Pagano;
Enhanced destruction of lymphoid cell lines by peripheral blood leukocytes taken from patients with acute infectious mononucleosis.
J. Immunol. 115:243-248(1975)

PubMed=62390; DOI=10.1111/j.1365-3083.1976.tb03029.x
William J. Yount, Peter D. Utsinger, Lindsey M. Hutt, Philip D. Buchanan, Joseph H. Korn, C. Randall Fuller, Marshall J. Logue, Joseph S. Pagano;
Subpopulations of human lymphoblastoid cell lines. Correlation with the expression of surface receptors and content of Epstein-Barr virus genome.
Scand. J. Immunol. 5:795-810(1976)

PubMed=1086134
Joseph Kaplan, Ward Davis Peterson Jr.;
Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.
Cancer Res. 36:3471-3475(1976)

PubMed=6600440; DOI=10.1007/BF02617996
Christel H. Uittenbogaart, Yael Cantor, John L. Fahey;
Growth of human malignant lymphoid cell lines in serum-free medium.
In Vitro 19:67-72(1983)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720
Michael Jules Mattes, Carlos Cordon-Cardo, John L. Lewis Jr., Lloyd John Old, Kenneth Oliver Lloyd;
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 Carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3495441; DOI=10.1002/eji.1830170503
Susan Kanowith-Klein, Andrew Saxon, Christel H. Uittenbogaart;
Constitutive production of B cell differentiation factor-like activity by human T and B cell lines.
Eur. J. Immunol. 17:593-598(1987)

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x
Akinobu Nakano, Takayuki Harada, Shigeru Morikawa, Yuzuru Kato;
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Acta Pathol. Jpn. 40:107-115(1990)

PubMed=2144611; DOI=10.1128/mcb.10.10.5502-5509.1990; PMCID=PMC361264
Jian Cheng, Martin Haas;
Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines.
Mol. Cell. Biol. 10:5502-5509(1990)

PubMed=2255914; DOI=10.1126/science.2255914
Peter D. Aplan, Donald P. Lombardi, Ann M. Ginsberg, Jeffrey Cossman, Virginia L. Bertness, Ilan R. Kirsch;
Disruption of the human SCL locus by 'illegitimate' V-(D)-J recombinase activity.
Science 250:1426-1429(1990)

CLPUB00447
Richard A. Mulivor, Sharon F. Suchy;
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In misc. document) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda; USA (1992)

PubMed=8316623; DOI=10.2307/3578190
Helen Harrington Evans, Marlene Ricanati, Min-Fen Horng, Qiao-Yun Jiang, Jaroslav Mencl, Peggy Louise Olive;
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7630190
Mu-Xiang Zhou, Lu-Bing Gu, Charles David James, J. He, Andrew M. Yeager, Stephen D. Smith, Harry W. Findley Jr.;
Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia.
Leukemia 9:1159-1161(1995)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Reiner Siebert, Christoph P. Willers, Alexander Schramm, Alexander Fossa, Irina M. Gana Dresen, Michael J. Uppenkamp, Mohammad Resa Nowrousian, Siegfried Seeber, Bertram Opalka;
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

DOI=10.11418/jtca1981.15.4_211
Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura, Tadao Ohno;
Identification of cell lines with variable numbers of tandem repeat (VNTR) amplified by polymerase chain reaction.
Tissue Cult. Res. Commun. 15:211-219(1996)

PubMed=8558913
S. Morita, S. Tsuchiya, Hiromi Fujie, Masayuki Itano, Yoshiyuki Ohashi, Masayoshi Minegishi, Masue Imaizumi, M. Endo, Nagako Takano, Tasuke Konno;
Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.
Leukemia 10:102-105(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998
Nobuhiro Kawasaki, Yoshinobu Matsuo, Tadashi Yoshino, Hiroyuki Yanai, Takashi Oka, Norihiro Teramoto, Cao Liu, Eisaku Kondo, Jun Minowada, Tadaatsu Akagi;
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9787181; DOI=10.1182/blood.V92.9.3410
Akira Sakai, Catherine Thieblemont, Axel Wellmann, Elaine Sarkin Jaffe, Mark Raffeld;
PTEN gene alterations in lymphoid neoplasms.
Blood 92:3410-3415(1998)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Renate Burger, Thomas E. Hansen-Hagge, Hans Gunther Drexler, Martin Gramatzki;
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Leuk. Res. 25:275-278(2001)

PubMed=14504097; DOI=10.1182/blood-2003-02-0418
Takeshi Taketani, Tomohiko Taki, Kanji Sugita, Yoshiyuki Furuichi, Eiichi Ishii, Ryoji Hanada, Masahiro Tsuchida, Kenichi Sugita, Kohmei Ida, Yasuhide Hayashi;
FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.
Blood 103:1085-1088(2004)

PubMed=15472075; DOI=10.1126/science.1102160
Andrew P. Weng, Adolfo A. Ferrando, Woojoong Lee, John P. Morris 4th, Lewis B. Silverman, Cheryll Sanchez-Irizarry, Stephen Charles Blacklow, Alfred Thomas Look, Jon Christopher Aster;
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Yoshihiro Murai, Shinichi Hayashi, Hiroyuki Takahashi, Koichi Tsuneyama, Yasuo Takano;
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=17117183; DOI=10.1038/sj.bjc.6603447; PMCID=PMC2360743
Alex H. Beesley, Misty-Lee Palmer, Jette Ford, Richard E. Weller, Aaron J. Cummings, Joseph R. Freitas, Martin J. Firth, Kanchana U. Perera, Nick H. de Klerk, Ursula R. Kees;
Authenticity and drug resistance in a panel of acute lymphoblastic leukaemia cell lines.
Br. J. Cancer 95:1537-1544(2006)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Yorick Sandberg, Brenda Verhaaf, Ellen J. van Gastel-Mol, Ingrid L.M. Wolvers-Tettero, John De Vos, Roderick A.F. MacLeod, Jeroen G. Noordzij, Willem A. Dik, Jack J.M. van Dongen, Anton W. Langerak;
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=22675565; DOI=10.1371/journal.pone.0038463; PMCID=PMC3366948
Zeynep Kalender Atak, Kim De Keersmaecker, Valentina Gianfelici, Ellen Geerdens, Roel Vandepoel, Daphnie Pauwels, Michael Porcu, Idoya Lahortiga, Vanessa Brys, Wilhelm Gerhard Dirks, Hilmar Quentmeier ...Show all 17 authors... , Jacqueline Cloos, Harry Cuppens, Anne Uyttebroeck, Peter Vandenberghe, Jan Cools, Stein Aerts; Show fewer authors
High accuracy mutation detection in leukemia on a selected panel of cancer genes.
PLoS ONE 7:e38463.1-e38463.11(2012)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5; PMCID=PMC8967900
Isabelle Rose Leo, Luay Aswad, Matthias Stahl, Elena Kunold, Frederik Post, Tom Erkers, Nona Struyf, Georgios Mermelekas, Rubin Narayan Joshi, Eva Gracia-Villacampa, Paivi Ostling ...Show all 17 authors... , Olli-Pekka Kallioniemi, Katja Pokrovskaja Tamm, Ioannis Siavelis, Janne Lehtio, Mattias Vesterlund, Rozbeh Jafari; Show fewer authors
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

Cross-references
Cell line collections (Providers) ATCC; CCL-120.1
BEI_Resources; ARP-497
CLS; 300214 - Discontinued
Coriell; GM04155
DSMZ; ACC-435
ECACC; 85112801
IZSLER; BS TCL 185
JCRB; JCRB0031
KCLB; 10120.1
NCBI_Iran; C530
RCB; RCB0016
Cell line databases/resources CLO; CLO_0002335
CLO; CLO_0004258
CLO; CLO_0016177
CLO; CLO_0050971
CLDB; cl678
cancercelllines; CVCL_1859
Cell_Model_Passport; SIDM01558
DSMZCellDive; ACC-435
LINCS_LDP; LCL-1914
Anatomy/cell type resources BTO; BTO_0005370
Biological sample resources BioSample; SAMN03472334
BioSample; SAMN03472701
BioSample; SAMN03473542
Chemistry resources ChEMBL-Cells; CHEMBL3307642
ChEMBL-Targets; CHEMBL614541
PubChem_Cell_line; CVCL_1859
Encyclopedic resources Wikidata; Q54809037
Experimental variables resources EFO; EFO_0022508
Gene expression databases GEO; GSM5137752
Polymorphism and mutation databases Cosmic; 683532
Cosmic; 801729
Cosmic; 850195
Cosmic; 913416
Cosmic; 922679
Cosmic; 932768
Cosmic; 996307
Cosmic; 998723
Cosmic; 1037688
Cosmic; 1115591
Cosmic; 1175128
Cosmic; 1226856
Cosmic; 1281369
Cosmic; 1330497
Cosmic; 1524797
Cosmic; 1641390
Cosmic; 1760523
Cosmic; 2165712
Cosmic; 2361365
Cosmic; 2458762
Cosmic; 2462861
Cosmic; 2602915
Cosmic; 2649236
IARC_TP53; 21649
Proteomic databases PRIDE; PXD023662
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number44