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Cellosaurus OCI-AML-3 (CVCL_1844)

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Cell line name OCI-AML-3
Synonyms OCI-Aml-3; OCI/AML-3; OCI-AML3; OCI/AML3; OCI AML3; OCIAML3; Ontario Cancer Institute-Acute Myeloid Leukemia-3
Accession CVCL_1844
Resource Identification Initiative To cite this cell line use: OCI-AML-3 (RRID:CVCL_1844)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: LL-100 blood cancer cell line panel.
Population: Caucasian.
Doubling time: 28 hours (PubMed=25984343); ~35-40 hours (DSMZ=ACC-582).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Phenotyping; shRNA library screening.
Omics: Proteomics; PTM; Phosphorylation; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Mutation; HGNC; HGNC:2978; DNMT3A; Simple; p.Arg882Cys (c.2644C>T); ClinVar=VCV000375882; Zygosity=Heterozygous (PubMed=26434589; PubMed=31160637; PubMed=31739141; Cosmic-CLP=1290455; DepMap=ACH-000336).
  • Mutation; HGNC; HGNC:7910; NPM1; Simple; p.Trp288Cysfs*12 (c.860_863dupTCTG); ClinVar=VCV000013998; Zygosity=Heterozygous (PubMed=16079892).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Homozygous (Cosmic-CLP=1290455; DepMap=ACH-000336).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,23:01
HLA-BB*44:02,53:01
HLA-CC*04:01,05:01

Source: DSMZCellDive=ACC-582
Class I
HLA-AA*02:01:01,23:01:01
HLA-BB*44:02:01,53:01:01
HLA-CC*04:01:01,05:01:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American1.44
East Asian, North2.86
East Asian, South0
South Asian0.99
European, North55.94
European, South38.78
Disease Adult acute myelomonocytic leukemia (NCIt: C7962)
Acute myelomonocytic leukemia (ORDO: Orphanet_517)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_D6C9 (HyCyte OCI-AML3 KO-hFGL2)CVCL_D6NG (OCI-AML3/HaABCG2 clone 3.3)CVCL_5227 (OCI-AML3/HaABCG2 clone 6.2)
CVCL_D6NF (OCI-AML3DNR)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=1290455; DSMZ=ACC-582; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D2S133817,21
D3S135816
D5S81811,13
D7S8209,10
D8S117910,12
D13S3178,13
D16S53911,13
D18S5112,18
D19S43315,17
D21S1129,30
FGA22
Penta D12,13
Penta E11,13
TH018,9.3 (DSMZ=ACC-582)
8,10 (Cosmic-CLP=1290455; PubMed=25877200)
TPOX8,11
vWA17,18

Run an STR similarity search on this cell line
Publications

PubMed=2538684
Chen Wang, John E. Curtis, Mark David Minden, Ernest Armstrong McCulloch;
Expression of a retinoic acid receptor gene in myeloid leukemia cells.
Leukemia 3:264-269(1989)

PubMed=12393637; DOI=10.1182/blood-2002-01-0271
Brian L. Abbott, Anne-Marie Colapietro, Yu-Xiao Elizabeth Barnes, Frank C. Marini, Michael Andreeff, Brian P. Sorrentino;
Low levels of ABCG2 expression in adult AML blast samples.
Blood 100:4594-4601(2002)

PubMed=16079892; DOI=10.1038/sj.leu.2403899
Hilmar Quentmeier, Maria Paola Martelli, Wilhelm Gerhard Dirks, Niccolo Bolli, Arcangelo Liso, Roderick A.F. MacLeod, Ines Nicoletti, Roberta Mannucci, Alessandra Pucciarini, Barbara Bigerna, Massimo Fabrizio Martelli ...Show all 14 authors... , Cristina Mecucci, Hans Gunther Drexler, Brunangelo Falini; Show fewer authors
Cell line OCI/AML3 bears exon-12 NPM gene mutation-A and cytoplasmic expression of nucleophosmin.
Leukemia 19:1760-1767(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Hilmar Quentmeier, Roderick A.F. MacLeod, Margarete Zaborski, Hans Gunther Drexler;
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=21552520; DOI=10.1371/journal.pone.0019169; PMCID=PMC3084268
Ting-Lei Gu, Julie Nardone, Yi Wang, Marc Loriaux, Judit Villen, Sean A. Beausoleil, Meghan Tucker, Jon M. Kornhauser, Jian-Min Ren, Joan MacNeill, Steven P. Gygi ...Show all 15 authors... , Brian Jay Druker, Michael Charles Heinrich, John Rush, Roberto D. Polakiewicz; Show fewer authors
Survey of activated FLT3 signaling in leukemia.
PLoS ONE 6:e19169.1-e19169.10(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26434589; DOI=10.1038/onc.2015.359; PMCID=PMC4705435
Humberto J. Ferreira, Holger Heyn, Miguel Vizoso, Catia Moutinho, Enrique Vidal, Antonio Gomez, Anna Martinez-Cardus, Laia Simo-Riudalbas, Sebastian Moran, Edgar Jost, Manel Esteller;
DNMT3A mutations mediate the epigenetic reactivation of the leukemogenic factor MEIS1 in acute myeloid leukemia.
Oncogene 35:3079-3082(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28109323; DOI=10.1186/s13045-017-0396-0; PMCID=PMC5251306
Riccardo Masetti, Salvatore Nicola Bertuccio, Annalisa Astolfi, Francesca Chiarini, Annalisa Lonetti, Valentina Indio, Matilde De Luca, Jessica Bandini, Salvatore Serravalle, Monica Franzoni, Martina Pigazzi ...Show all 15 authors... , Alberto Maria Martelli, Giuseppe Basso, Franco Locatelli, Andrea Pession; Show fewer authors
Hh/Gli antagonist in acute myeloid leukemia with CBFA2T3-GLIS2 fusion gene.
J. Hematol. Oncol. 10:26.1-26.5(2017)

PubMed=29491412; DOI=10.1038/s41388-018-0150-2; PMCID=PMC5955861
Daniel Gerlach, Ulrike Tontsch-Grunt, Anke Baum, Johannes Popow, Dirk Scharn, Marco H. Hofmann, Harald Engelhardt, Onur Kaya, Janina Beck, Norbert Schweifer, Thomas Gerstberger ...Show all 14 authors... , Johannes Zuber, Fabio Savarese, Norbert Kraut; Show fewer authors
The novel BET bromodomain inhibitor BI 894999 represses super-enhancer-associated transcription and synergizes with CDK9 inhibition in AML.
Oncogene 37:2687-2701(2018)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Kar-Tong Tan, Ling-Wen Ding, Qiao-Yang Sun, Zhen-Tang Lao, Wenwen Chien, Xi Ren, Jin-Fen Xiao, Xin-Yi Loh, Liang Xu, Michael Lill, Anand Mayakonda ...Show all 14 authors... , De-Chen Lin, Henry He Yang, Harold Phillip Koeffler; Show fewer authors
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Cord C. Uphoff, Claudia Pommerenke, Sabine A. Denkmann, Hans Gunther Drexler;
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:e0210404.1-e0210404.22(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x; PMCID=PMC6547646
Hilmar Quentmeier, Claudia Pommerenke, Wilhelm Gerhard Dirks, Sonja Eberth, Max Koeppel, Roderick A.F. MacLeod, Stefan Nagel, Klaus G. Steube, Cord C. Uphoff, Hans Gunther Drexler;
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218.1-8218.14(2019)

PubMed=31739141; DOI=10.1016/j.leukres.2019.106270
Hilmar Quentmeier, Claudia Pommerenke, Wilhelm Gerhard Dirks, Silke Fahnrich, Vivien Hauer, Cord C. Uphoff, Margarete Zaborski, Hans Gunther Drexler;
DNMT3A R882H mutation in acute myeloid leukemia cell line SET-2.
Leuk. Res. 88:106270.1-106270.3(2020)

PubMed=36982453; DOI=10.3390/ijms24065377; PMCID=PMC10049680
Rafal Skopek, Malgorzata Palusinska, Katarzyna Kaczor-Keller, Rafal Pingwara, Anna Papierniak-Wygladala, Tino Schenk, Slawomir Lewicki, Artur Zelent, Lukasz Szymanski;
Choosing the right cell line for acute myeloid leukemia (AML) research.
Int. J. Mol. Sci. 24:5377.1-5377.34(2023)

Cross-references
Cell line collections (Providers) CCTCC; GDC0216
DSMZ; ACC-582
Ubigene; YC-C022
Cell line databases/resources CLO; CLO_0009853
cancercelllines; CVCL_1844
Cell_Model_Passport; SIDM00462
Cosmic-CLP; 1290455
DepMap; ACH-000336
DSMZCellDive; ACC-582
LINCS_LDP; LCL-1066
Lonza; 530
Anatomy/cell type resources BTO; BTO_0004620
Biological sample resources BioSample; SAMN03473107
BioSample; SAMN10988470
CRISP screens repositories BioGRID_ORCS_Cell_line; 214
Chemistry resources ChEMBL-Cells; CHEMBL4295392
ChEMBL-Targets; CHEMBL4296479
GDSC; 1290455
PharmacoDB; OCIAML3_1184_2019
PubChem_Cell_line; CVCL_1844
Encyclopedic resources Wikidata; Q54931742
Experimental variables resources EFO; EFO_0006289
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM482512
GEO; GSM887469
GEO; GSM888549
GEO; GSM1446756
GEO; GSM1525022
GEO; GSM1670296
GEO; GSM2716711
GEO; GSM2716712
GEO; GSM2716774
GEO; GSM2716775
GEO; GSM2716776
GEO; GSM2716777
Polymorphism and mutation databases Cosmic; 787467
Cosmic; 975289
Cosmic; 1012110
Cosmic; 1127258
Cosmic; 1245831
Cosmic; 1319546
Cosmic; 1557602
Cosmic; 1582387
Cosmic; 1696140
Cosmic; 1779129
Cosmic; 2131552
Cosmic; 2306232
Cosmic; 2750869
IARC_TP53; 30132
LiGeA; CCLE_569
Progenetix; CVCL_1844
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number46