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Cellosaurus MKN28 (CVCL_1416)

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Cell line name MKN28
Synonyms MKN-28; MKN 28
Accession CVCL_1416
Resource Identification Initiative To cite this cell line use: MKN28 (RRID:CVCL_1416)
Comments Problematic cell line: Contaminated. Shown to be a MKN74 derivative (PubMed=20143388).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: JFCR39 cancer cell line panel.
Part of: JFCR45 cancer cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00328.
Population: Japanese.
Doubling time: 28-32 hours (PubMed=3962675); 28 hours (PubMed=29435981); 30-34 hours (CelloPub=CLPUB00584).
Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Liver; UBERON=UBERON_0002107.
Sequence variations
HLA typing Source: PubMed=9023415
Class I
HLA-AA*31:01
HLA-BB*51:01
HLA-CC*03:04

Source: PubMed=25960936
Class I
HLA-BB*51:01
HLA-CC*03:04,03:04
Class II
HLA-DRDRB1*15:01,15:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North83.28
East Asian, South16.71
South Asian0
European, North0
European, South0
Disease Gastric tubular adenocarcinoma (NCIt: C5473)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2791 (MKN74)
Children:
CVCL_UW56 (MKN28-EGFP)CVCL_QZ55 (RMKN-28)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=908139; JCRB=JCRB0253; KCLB=80102; RCB=RCB1000

Markers:
AmelogeninX
CSF1PO12
D3S135816
D5S81811
D7S8209
D13S31711
D16S5399,11
FGA23
TH016
TPOX8,11
vWA16,20

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; JCRB; -; https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
Publications

CLPUB00581
Haruto Hojo;
Establishment of cultured cell lines of human stomach cancer origin and their morphological characteristics.
Niigata Igakkai Zasshi 91:737-763(1977)

CLPUB00584
Teiichi Motoyama, Haruto Hojo, Toshimitsu Suzuki, Shoichi Oboshi;
Evaluation of the regrowth assay method as an in vitro drug sensitivity test and its application to cultured human gastric cancer cell lines.
Acta Med. Biol. 27:49-63(1979)

PubMed=758928; DOI=10.1038/bjc.1979.3; PMCID=PMC2009802
Mitsuru Kinjo, Kazunari Oka, S. Naito, Shin Kohga, Kenzo Tanaka, Shoichi Oboshi, Yoshihiro Hayata, Kosei Yasumoto;
Thromboplastic and fibrinolytic activities of cultured human cancer cell lines.
Br. J. Cancer 39:15-23(1979)

PubMed=6862145; DOI=10.20772/cancersci1959.74.2_240
Seiji Naito, Sadami Inoue, Mitsuru Kinjo, Kenzo Tanaka;
Thromboplastic and fibrinolytic activities of cultured human gastric cancer cell lines.
Gann 74:240-247(1983)

PubMed=3986962; DOI=10.1093/carcin/6.4.549
Masahiro Watatani, Mituo Ikenaga, Toshihiro Hatanaka, Masakatsu Kinuta, Shin-ichiro Takai, Takesada Mori, Sohei Kondo;
Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.
Carcinogenesis 6:549-553(1985)

PubMed=3962675; DOI=10.1111/j.1440-1827.1986.tb01461.x
Teiichi Motoyama, Haruto Hojo, Hidenobu Watanabe;
Comparison of seven cell lines derived from human gastric carcinomas.
Acta Pathol. Jpn. 36:65-83(1986)

PubMed=1933850
Yukishige Yamada, Teruhiko Yoshida, Kenshi Hayashi, Takao Sekiya, Jun Yokota, Setsuo Hirohashi, Katsunori Nakatani, Hiroshige Nakano, Takashi Sugimura, Masaaki Terada;
p53 gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases.
Cancer Res. 51:5800-5805(1991)

PubMed=1370612; DOI=10.1016/S0006-291X(05)80133-0
Takashi Matozaki, Choitsu Sakamoto, Kohei Matsuda, Toshiya Suzuki, Yoshitaka Konda, Osamu Nakano, Ken Wada, Tohru Uchida, Hogara Nishisaki, Munehiko Nagao, Masato Kasuga;
Missense mutations and a deletion of the p53 gene in human gastric cancer.
Biochem. Biophys. Res. Commun. 182:215-223(1992)

DOI=10.1016/B978-0-12-333530-2.50014-9
Morimasa Sekiguchi, Toshimitsu Suzuki;
Gastric tumor cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.287-316; Academic Press; New York; USA (1994)

PubMed=9023415; DOI=10.1006/cimm.1996.1062
Naoko Seki, Tomoaki Hoshino, Megumi Kikuchi, Akihiro Hayashi, Kyogo Itoh;
HLA-A locus-restricted and tumor-specific CTLs in tumor-infiltrating lymphocytes of patients with non-small cell lung cancer.
Cell. Immunol. 175:101-110(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=11107048; DOI=10.1046/j.1440-1827.2000.01117.x
Hiroshi Yokozaki;
Molecular characteristics of eight gastric cancer cell lines established in Japan.
Pathol. Int. 50:767-777(2000)

PubMed=11314020; DOI=10.1038/sj.onc.1204160
Hiromi Kataoka, Yutaka Miura, Takashi Joh, Kyoji Seno, Toyohiro Tada, Taiki Tamaoki, Hidekazu Nakabayashi, Makoto Kawaguchi, Kiyofumi Asai, Taiji Kato, Makoto Itoh;
Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1.
Oncogene 20:869-873(2001)

PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x; PMCID=PMC11160020
Hisashi Takada, Issei Imoto, Hitoshi Tsuda, Itaru Sonoda, Takashi Ichikura, Hidetaka Mochizuki, Takeshi Okanoue, Johji Inazawa;
Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
Cancer Sci. 96:100-110(2005)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Noriyuki Nakatsu, Yoko Yoshida, Kanami Yamazaki, Tomoki Nakamura, Shingo Dan, Yasuhisa Fukui, Takao Yamori;
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=15900046; DOI=10.1093/jnci/dji133
Tetsuo Mashima, Tomoko Oh-hara, Shigeo Sato, Mikiko Mochizuki, Yoshikazu Sugimoto, Kanami Yamazaki, Jun-ichi Hamada, Mitsuhiro Tada, Tetsuya Moriuchi, Yuichi Ishikawa, Yo Kato ...Show all 14 authors... , Hiroshi Tomoda, Takao Yamori, Takashi Tsuruo; Show fewer authors
p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
J. Natl. Cancer Inst. 97:765-777(2005)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Yoshihiro Murai, Shinichi Hayashi, Hiroyuki Takahashi, Koichi Tsuneyama, Yasuo Takano;
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
De-Xin Kong, Takao Yamori;
JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
Bioorg. Med. Chem. 20:1947-1951(2012)

PubMed=22490663; DOI=10.1016/j.bbrc.2012.03.122
Yuriko Saiki, Yuki Yoshino, Hiroko Fujimura, Tatsuya Manabe, Yuki Kudo, Miki Shimada, Nariyasu Mano, Tomohiro Nakano, Yoonha Lee, Shinjiro Shimizu, Shinya Oba ...Show all 23 authors... , Sho Fujiwara, Hideyuki Shimizu, Na Chen, Zhaleh Kashkouli Nezhad, Guo Jin, Shinichi Fukushige, Makoto Sunamura, Masaharu Ishida, Fuyuhiko Motoi, Shinichi Egawa, Michiaki Unno, Akira Horii; Show fewer authors
DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells.
Biochem. Biophys. Res. Commun. 421:98-104(2012)

PubMed=23671654; DOI=10.1371/journal.pone.0063056; PMCID=PMC3646030
Yu-Heng Lu, T. David Soong, Olivier Elemento;
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:e63056.1-e63056.10(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893; PMCID=PMC4355981
Sebastian Boegel, Martin Lower, Thomas Bukur, Ugur Sahin, John C. Castle;
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29435981; DOI=10.1002/ijc.31304
Hyun Jeong Kim, Sun Kyoung Kang, Woo Sun Kwon, Tae Soo Kim, Inhye Jeong, Hei-Cheul Jeung, Michael Kragh, Ivan D. Horak, Hyun Cheol Chung, Sun Young Rha;
Forty-nine gastric cancer cell lines with integrative genomic profiling for development of c-MET inhibitor.
Int. J. Cancer 143:151-159(2018)

PubMed=29717028; DOI=10.1042/BSR20180277; PMCID=PMC6050193
Ye-Qian Zhang, Si-Te Yu, Zi-Zhen Zhang, Gang Zhao, Jia Xu;
Long non-coding RNA AK096174 promotes cell proliferation and invasion in gastric cancer by regulating WDR66 expression.
Biosci. Rep. 38:BSR20180277.1-BSR20180277.12(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ABM; T9192
JCRB; FDSC0011 - Discontinued
JCRB; JCRB0253
JCRB; NIHS0324 - Discontinued
JCRB; NIHS0325 - Discontinued
KCLB; 80102
RCB; RCB1000 - Discontinued
Cell line databases/resources CLO; CLO_0037142
cancercelllines; CVCL_1416
Cell_Model_Passport; SIDM00260
CGH-DB; 119-1
CGH-DB; 9021-4
Cosmic-CLP; 908139
DepMap; ACH-002161
LINCS_LDP; LCL-1922
Anatomy/cell type resources BTO; BTO_0002381
Biological sample resources BioSample; SAMN03151847
CRISP screens repositories BioGRID_ORCS_Cell_line; 944
Chemistry resources ChEMBL-Cells; CHEMBL3307602
ChEMBL-Targets; CHEMBL614353
GDSC; 908139
PharmacoDB; MKN28_937_2019
PubChem_Cell_line; CVCL_1416
Encyclopedic resources Wikidata; Q54905823
Experimental variables resources EFO; EFO_0002831
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM562396
GEO; GSM1670112
Polymorphism and mutation databases Cosmic; 801338
Cosmic; 848146
Cosmic; 848371
Cosmic; 873710
Cosmic; 875905
Cosmic; 877041
Cosmic; 887249
Cosmic; 888826
Cosmic; 897534
Cosmic; 908139
Cosmic; 918496
Cosmic; 922694
Cosmic; 926113
Cosmic; 968348
Cosmic; 983726
Cosmic; 1066230
Cosmic; 1067208
Cosmic; 1090460
Cosmic; 1093298
Cosmic; 1223588
Cosmic; 1436025
Cosmic; 1460710
Cosmic; 1482081
Cosmic; 1627264
Cosmic; 1995509
Cosmic; 2036660
Cosmic; 2069778
Cosmic; 2823226
IARC_TP53; 972
IARC_TP53; 21505
Progenetix; CVCL_1416
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number45