Cellosaurus logo
expasy logo

Cellosaurus Daoy (CVCL_1167)

[Text version]
Cell line name Daoy
Synonyms DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324
Accession CVCL_1167
Secondary accession CVCL_7903
Resource Identification Initiative To cite this cell line use: Daoy (RRID:CVCL_1167)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 33.6 hours (Note=At 48th passage) (PubMed=2993532); 32 +- 1.5 hours (PubMed=31346954); 34 hours (ATCC=HTB-186).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; 2DE-MS.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Brain, posterior fossa; UBERON=UBERON_0008788.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (ATCC=HTB-186).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys242Phe (c.725G>T); ClinVar=VCV000376578; Zygosity=Homozygous (PubMed=22343310; ATCC=HTB-186; Cosmic-CLP=906833; DepMap=ACH-000211).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.45
Native American0
East Asian, North1.54
East Asian, South0
South Asian1.1
European, North64.36
European, South32.54
Disease Medulloblastoma, SHH-activated, TP53-mutant (NCIt: C129442)
Medulloblastoma (ORDO: Orphanet_616)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C8V5 (Daoy EZHIP KO)CVCL_A9DF (Daoy-luc2-tdT)
Sex of cell Male
Age at sampling 4Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-186; CCRID; Cosmic-CLP=906833; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D2S133816,17
D3S135815
D5S81811,13
D7S8208,10
D8S117913,15
D13S31713,14
D16S53910
D18S5112
D19S43314,14.2 (ATCC=HTB-186; CCRID)
14.2 (PubMed=25877200)
D21S1129,31.2
FGA23
Penta D10,13
Penta E7,11
TH019
TPOX8,10
vWA14,20

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/d/cell-lines-detail-566.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/DAOY_(biology)
Publications

PubMed=2993532; DOI=10.1097/00005072-198509000-00003
Phillip F. Jacobsen, Dorothy Joy Jenkyn, John M. Papadimitriou;
Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice.
J. Neuropathol. Exp. Neurol. 44:472-485(1985)

PubMed=1822845
Carol J. Wikstrand, Henry Seth Friedman, Darell Doty Bigner;
Medulloblastoma cell-substrate interaction in vitro.
Invasion Metastasis 11:310-324(1991)

DOI=10.1016/B978-0-12-333530-2.50005-8
Monica Nister, Bengt Westermark;
Human glioma cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.17-42; Academic Press; New York; USA (1994)

DOI=10.1007/0-306-46861-1_11
Francis Ali-Osman;
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=12973738; DOI=10.1002/pmic.200300460
Andreas Peyrl, Kurt Krapfenbauer, Irene Slavc, Jae-Won Yang, Thomas Strobel, Gert Lubec;
Protein profiles of medulloblastoma cell lines DAOY and D283: identification of tumor-related proteins and principles.
Proteomics 3:1781-1800(2003)

PubMed=18773455; DOI=10.1002/cyto.a.20633
Vinit Krishna Srivastava, Josephine Nalbantoglu;
Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer stem-like phenotype.
Cytometry A 73:940-948(2008)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20847082; DOI=10.1093/neuonc/noq091; PMCID=PMC3018940
Natalia Teider, Deborah K. Scott, Adrianne Neiss, Shyamal Dilhan Weeraratne, Vladimir M. Amani, Yi-Fei Wang, Victor E. Marquez, Yoon-Jae Cho, Scott L. Pomeroy;
Neuralized1 causes apoptosis and downregulates Notch target genes in medulloblastoma.
Neuro-oncol. 12:1244-1256(2010)

PubMed=22343310; DOI=10.1016/j.neulet.2012.02.022
Sara Ghassemifar, Susan M. Mendrysa;
MDM2 antagonism by nutlin-3 induces death in human medulloblastoma cells.
Neurosci. Lett. 513:106-110(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22723427; DOI=10.1093/neuonc/nos130; PMCID=PMC3424207
Arend H. Sikkema, Wilfred F.A. den Dunnen, Esther Hulleman, Dannis G. van Vuurden, Guillermo Garcia-Manero, Hui Yang, Frank J.G. Scherpen, Kim Rosalie Kampen, Eelco W. Hoving, Willem A. Kamps, Sander H. Diks ...Show all 13 authors... , Maikel P. Peppelenbosch, Eveline S.J.M. de Bont; Show fewer authors
EphB2 activity plays a pivotal role in pediatric medulloblastoma cell adhesion and invasion.
Neuro-oncol. 14:1125-1135(2012)

PubMed=24297863; DOI=10.1158/1078-0432.CCR-13-2281; PMCID=PMC4198154
Pratiti Bandopadhayay, Guillaume Bergthold, Brian Nguyen, Simone Schubert, Sharareh Gholamin, Yu-Jie Tang, Sara Bolin, Steven E. Schumacher, Rhamy Zeid, Sabran Masoud, Fu-Rong Yu ...Show all 25 authors... , Nujsaubnusi Vue, William James Gibson, Brenton R. Paolella, Siddhartha Shankar Mitra, Samuel H. Cheshier, Jun Qi, Kun-Wei Liu, Robert J. Wechsler-Reya, William Andrew Weiss, Fredrik Johansson Swartling, Mark W. Kieran, James E. Bradner, Rameen Beroukhim, Yoon-Jae Cho; Show fewer authors
BET bromodomain inhibition of MYC-amplified medulloblastoma.
Clin. Cancer Res. 20:912-925(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27498314; DOI=10.1016/j.jbiotec.2016.07.028
Delyan P. Ivanov, Beth Coyle, David A. Walker, Anna M. Grabowska;
In vitro models of medulloblastoma: choosing the right tool for the job.
J. Biotechnol. 236:10-25(2016)

PubMed=27812533; DOI=10.1016/j.dib.2016.10.004; PMCID=PMC5079239
Delyan P. Ivanov, David A. Walker, Beth Coyle, Anna M. Grabowska;
Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines.
Data Brief 9:696-698(2016)

PubMed=29900115; DOI=10.1016/j.euprot.2016.03.015; PMCID=PMC5988510
Claudia Martelli, Luca D'Angelo, Marta Barba, Mirko Baranzini, Ilaria Inserra, Federica Iavarone, Federica Vincenzoni, Gianpiero Tamburrini, Luca Massimi, Concezio Di Rocco, Massimo Caldarelli ...Show all 16 authors... , Irene Messana, Fabrizio Michetti, Massimo Castagnola, Wanda Lattanzi, Claudia Desiderio; Show fewer authors
Top-down proteomic characterization of DAOY medulloblastoma tumor cell line.
EuPA Open Proteom. 12:13-21(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31346954; DOI=10.1007/s10616-019-00332-3; PMCID=PMC6787134
Ricardo Bonfim Silva, Karina Bezerra Salomao, Thais Valeria Costa de Andrade Pimentel, Camila Cristina Branquinho de Oliveira Menezes, Patricia Vianna Bonini Palma, Aparecida Maria Fontes;
Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines.
Cytotechnology 71:893-903(2019)

PubMed=31963405; DOI=10.3390/cancers12010226; PMCID=PMC7016648
Arianna Casciati, Mirella Tanori, Remi Manczak, Sofiane Saada, Barbara Tanno, Paola Giardullo, Elena Porcu, Elena Rampazzo, Luca Persano, Giampietro Viola, Claire Dalmay ...Show all 15 authors... , Fabrice Lalloue, Arnaud Pothier, Caterina Merla, Mariateresa Mancuso; Show fewer authors
Human medulloblastoma cell lines: investigating on cancer stem cell-like phenotype.
Cancers (Basel) 12:226.1-226.14(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; HTB-186
BCRJ; 0325
CLS; 305053
KCB; KCB 2010203YJ
NCBI_Iran; C519
Cell line databases/resources CLO; CLO_0002699
cancercelllines; CVCL_1167
CCRID; 1101HUM-PUMC000218
CCRID; 3101HUMSCSP509
Cell_Model_Passport; SIDM00887
Cosmic-CLP; 906833
DepMap; ACH-000211
LINCS_LDP; LCL-1576
Lonza; 1438
Anatomy/cell type resources BTO; BTO_0004328
Biological sample resources BioSample; SAMN03470909
BioSample; SAMN10988097
ENCODE; ENCBS055ILX
ENCODE; ENCBS812KQP
CRISP screens repositories BioGRID_ORCS_Cell_line; 687
Chemistry resources ChEMBL-Cells; CHEMBL3308510
ChEMBL-Targets; CHEMBL612519
GDSC; 906833
PharmacoDB; Daoy_282_2019
PubChem_Cell_line; CVCL_1167
Encyclopedic resources Wikidata; Q29511023
Experimental variables resources EFO; EFO_0005698
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM482332
GEO; GSM886972
GEO; GSM888041
GEO; GSM919361
GEO; GSM1669725
GEO; GSM1938719
Polymorphism and mutation databases Cosmic; 687773
Cosmic; 906833
Cosmic; 923654
Cosmic; 973262
Cosmic; 974281
Cosmic; 975027
Cosmic; 1404441
Cosmic; 1995383
Cosmic; 2362649
Cosmic; 2731177
IARC_TP53; 480
LiGeA; CCLE_339
Progenetix; CVCL_1167
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number45