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Cellosaurus ACN (CVCL_1068)

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Cell line name ACN
Accession CVCL_1068
Resource Identification Initiative To cite this cell line use: ACN (RRID:CVCL_1068)
Comments Problematic cell line: Probably contaminated. The STR profile of ACN and LN-464 are identical. SNP analysis (DepMap) also shows identity of both cell lines. We believe this is a melanoma cell line based on the presence of the BRAF V600E mutation as well as the RNASeq expression profile of LN-464 (DepMap) which clusters with that of melanoma patient samples. Originally thought to originate from the bone marrow of a 36-month old boy with a metastatic adrenal stage-IV neuroblastoma.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00623.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (Cosmic-CLP=906803; DepMap=ACH-002389).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Asn184fs*6 (c.545_546insA); Zygosity=Heterozygous (Cosmic-CLP=906803; DepMap=ACH-002389).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Phe113Ser (c.338T>C); ClinVar=VCV000823709; Zygosity=Homozygous (Cosmic-CLP=906803; DepMap=ACH-002389).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.3
Native American0.69
East Asian, North0
East Asian, South0.16
South Asian1.02
European, North70.67
European, South27.16
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B7TC (ACN/IFN-gamma)CVCL_B7TD (ACN/neo)
Originate from same individual CVCL_3962 ! LN-464
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=906803; ICLC=HTL96020

Markers:
AmelogeninX
CSF1PO11
D3S135816
D5S81812
D7S82010,13
D8S117912,13
D13S31711,13
D16S53911
D18S5116
D21S1128
FGA22
Penta D9
Penta E10,14
TH019
TPOX9,12
vWA14,17

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=2467885; DOI=10.1002/ijc.2910430421
Nicole Gross, Daniel Beck, Jacques Portoukalian, Simone Favre, Stefan Carrel;
New anti-GD2 monoclonal antibodies produced from gamma-interferon-treated neuroblastoma cells.
Int. J. Cancer 43:665-671(1989)

PubMed=1354203; DOI=10.1002/ijc.2910520116
Nicole Gross, Simone Favre, Daniel Beck, Manuela Meyer;
Differentiation-related expression of adhesion molecules and receptors on human neuroblastoma tissues, cell lines and variants.
Int. J. Cancer 52:85-91(1992)

PubMed=8509230; DOI=10.1002/ijc.2910540329
Donata Rimoldi, Pedro Romero, Stefan Carrel;
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Int. J. Cancer 54:527-528(1993)

DOI=10.1007/0-306-46872-7_2
Carol Jeanne Thiele;
Neuroblastoma.
(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.21-53; Kluwer Academic Publishers; New York; USA (1999)

PubMed=15150552; DOI=10.1038/sj.bjc.6601842; PMCID=PMC2409504
Irma Airoldi, Raffaella Meazza, Michela Croce, Emma Di Carlo, Tiziana Piazza, Claudia Cocco, Tommaso D'Antuono, Vito Pistoia, Silvano Ferrini, Maria Valeria Corrias;
Low-dose interferon-gamma-producing human neuroblastoma cells show reduced proliferation and delayed tumorigenicity.
Br. J. Cancer 90:2210-2218(2004)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

Cross-references
Cell line collections (Providers) ICLC; HTL96020
Cell line databases/resources CLO; CLO_0001655
CLDB; cl239
cancercelllines; CVCL_1068
Cell_Model_Passport; SIDM00057
Cosmic-CLP; 906803
DepMap; ACH-002389
LINCS_LDP; LCL-1982
Chemistry resources ChEMBL-Cells; CHEMBL3308148
ChEMBL-Targets; CHEMBL2366195
GDSC; 906803
PharmacoDB; ACN_55_2019
PubChem_Cell_line; CVCL_1068
Encyclopedic resources Wikidata; Q54608539
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1669594
Polymorphism and mutation databases Cosmic; 906803
IARC_TP53; 21170
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number35